Cancer Genomics Shared Resource

癌症基因组学共享资源

• 批准号: 10569617
• 负责人: Boris Pasche
• 金额: $ 3.81万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10569617
• 关键词: ATAC-seq; Acceleration; American Cancer Society; Area; Automation; Award; B-cell receptor repertoire sequencing; Baptist Church; Bioinformatics; Bioinformatics Shared Resource; CRISPR library; Cancer Center; Cancer Center Support Grant; Capital Financing; Cells; Chromatin; Clinical; Collaborations; Comprehensive Cancer Center; Contracts; Counseling; DNA; Data Analyses; Department of Defense; Dependence; Development; Doctor of Philosophy; Education; Enrollment; Environment; Epigenetic Process; Etiology; Experimental Designs; Fee-for-Service Plans; Fostering; Foundations; Funding; Gene Expression Profiling; Genes; Genetic; Genome; Genomics; Genomics Shared Resource; Government; Grant; Growth; Heterogeneity; Hour; Immune; Industry; Instruction; Interdisciplinary Education; Leadership; Libraries; Malignant Neoplasms; Malignant neoplasm of lung; Measures; Mediation; Methylation; Molecular; Multiomic Data; NCI Center for Cancer Research; Pharmaceutical Preparations; Postdoctoral Fellow; Preparation; Production; Proteomics; Publications; Records; Recurrence; Research; Research Design; Research Personnel; Research Support; Resolution; Resource Sharing; Robotics; S10 grant; Science; Services; Strategic Planning; Students; Technology; Training and Education; Treatment outcome; United States National Institutes of Health; anticancer research; base; behavioral outcome; cancer genomics; cancer health disparity; clinical predictors; computerized data processing; cost effective; data integration; data mining; epigenome; experience; forest; genome sciences; genome sequencing; health disparity; interdisciplinary collaboration; lectures; member; microbiome sequencing; multiple omics; next generation sequencing; precision medicine; precision oncology; programs; response; screening; sequencing platform; single cell sequencing; single-cell RNA sequencing; statistics; therapy resistant; transcriptome; transcriptome sequencing; transcriptomics; translational impact; tumor

CANCER GENOMICS SHARED RESOURCE (CGSR): PROJECT SUMMARY The overarching objective of the Cancer Genomics Shared Resource (CGSR)is to maximize the scientific impact of basic, translational, and clinical cancer research at the Wake Forest Baptist Comprehensive Cancer Center (WFBCCC). CGSR achieves this objective by providing rapid and cost-effective access to state-of-the-art next- generation sequencing (NGS) technologies and services, while promoting interdisciplinary collaboration and education in genomic sciences. CGSR services support a range of research proje cts chiefly directed at the discovery and characterization of genomic, transcriptomic, and/or epigenetic alterations that: (1) underlie cancer formation and progression; (2) predict clinical cancer behavior and treatment outcomes; (3) define inter- and intra-tumoral molecular and clonal heterogeneity; and (4) impact tumor-immune and drug-response interactions. CGSR prioritizes cancer-related funded research, creates an optimal environment for the rapidly developing Cancer Center’s Precision Oncology Initiative, as set forth in its Strategic Plan, and partners with other shared resources to promote integrated cross-shared resource service workflows for advanced genomic applications, such as single-cell sequencingand spatial single-cell gene expression profiling. CGSR is co-led by Lance Miller, MS, PhD and GregoryHawkins, PhD, who have established records in cancer genomics research and funding. CGSR is operated by four full-time technicians with advanced genomics expertise. To expedite data processing and analysis, a staff bioinformatician is shared between the CGSR and the Bioinformatics Shared Resource. During the current funding cycle, CGSR has expanded from2.5 to 4.0 full-time technicians, grown its sequencing services by more than 3-fold, established single-cell sequencing platforms, introduced automation of NGS library preparation, upgraded NGS sequencing capacity, raised its user base from 39 to 57 WFBCCC members, and coordinated multiple successful collaborations with other WFBCCC Shared Resources. Du ring the current funding cycle (11/01/15–10/31/20), CGSR provided genomic services to 92 investigators, 57 (62%) of whom were WFBCCC members. Notably, 70% of CGSR’s total service capacity (measured by total staff -hours) was utilized by WFBCCC members. CGSR services directly supported 34 cancer-focused publications and contributed to the successful award of 22 NCI grants (12 R01s, 5 R21s, 2 R37s, 1 R33, 1 U01, and 1 K22) and cancer-focused grants from the Department of Defense (five), American Cancer Society (two), and other foundations (five).

癌症基因组共享资源（CGSR）：项目摘要 癌症基因组学共享资源（CGSR）的总体目标是最大化科学影响 Wake Forest Baptist综合癌症中心的基本，翻译和临床癌症研究 （WFBCCC）。 CGSR通过提供快速且具有成本效益的最新访问来实现这一目标 生成测序（NGS）技术和服务，同时促进跨学科的合作和 基因组科学教育。 CGSR服务支持一系列研究项目CTS主要针对 基因组，转录组和/或表观遗传学改变的发现和表征：（1）癌症基础 形成和进展； （2）预测临床癌症行为和治疗结果； （3）定义 - 肿瘤内分子和克隆异质性； （4）影响肿瘤免疫和药物反应相互作用。 CGSR优先考虑与癌症相关的资助研究，为快速发展创造了一个最佳环境 癌症中心的精确肿瘤学计划，如其战略计划中规定的 用于促进高级基因组应用的综合交叉共享资源服务工作流的资源， 例如单细胞测序和空间单细胞基因表达分析。 CGSR由Lance Miller共同领导 MS，PhD和Gregoryhawkins博士，他们在癌症基因组学研究和资金方面建立了记录。 CGSR由具有高级基因组专业知识的四名全职技术人员运营。加快数据处理 和分析，CGSR与生物信息学共享资源之间共享了一名生物信息学家。 在当前的融资周期中，CGSR已从2.5扩展到4.0全职技术人员，并成长 由3倍以上，已建立的单细胞测序平台提供的服务，引入了NGS库的自动化 准备工作，升级的NGS测序能力，将其用户群从39升至57个WFBCCC成员，并且 与其他WFBCCC共享资源协调了多次成功合作。电流 资金周期（11/01/15–10/31/20），CGSR为92名调查人员提供了基因组服务，其中57（62％） 是WFBCCC成员。值得注意的是，CGSR总服务能力的70％（由员工总数衡量）是 由WFBCCC成员使用。 CGSR服务直接支持34个以癌症为中心的出版物和 成功获得了22个NCI赠款（12 R01，5 R21，2 R37，1 R33、1 U01和1 K22）和1 K22）和 国防部（五），美国癌症协会（两个）等人以癌症为重点的赠款 基金会（五）。