Core D: Research Support Core

核心 D：研究支持核心

• 批准号: 10559682
• 负责人: Dean P Edwards
• 金额: $ 11.76万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-28 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10559682
• 关键词: Animals; Antibodies; Aromatic Hydrocarbons; Aromatic Polycyclic Hydrocarbons; Award; Biological Assay; Bronchopulmonary Dysplasia; Cancer Center Support Grant; ChIP-seq; Chromatin; Chronic lung disease; Clinical; Communities; Computers and Advanced Instrumentation; Consultations; Core Facility; DNA Methylation; Data; Data Analyses; Data Set; Detection; Development; Dose; Educational workshop; Ensure; Enzymes; Epigenetic Process; Experimental Designs; Experimental Models; Exposure to; Faculty; Funding; Genetic; Genomics; Goals; Grant; Human; Individual; Infrastructure; Institution; Mass Fragmentography; Mass Spectrum Analysis; Measures; Mentors; Metabolic; Methods; Molecular; Molecular Profiling; Neurocognitive Deficit; Outcome; Oxidation-Reduction; Phase; Postdoctoral Fellow; Pregnant Women; Premature Birth; Prevention; Procedures; Progress Reports; Protein Array; Proteomics; Protocols documentation; Research; Research Institute; Research Personnel; Research Project Summaries; Research Support; Resources; Rice; Services; Signal Recognition Particle; Site; Superfund; Techniques; Technology; Training; Validation; Work; analytical method; anticancer research; biomarker identification; biomarker signature; bisulfite sequencing; cancer prevention; data management; design; doctoral student; early life exposure; epigenetic profiling; epigenomics; experience; genome sequencing; genome-wide; genomic platform; graduate student; high risk; histone modification; investigator training; metabolomics; multiple omics; neurobehavioral; novel; organizational structure; postnatal; prenatal; preterm newborn; remediation; skills; superfund site; supplemental oxygen; technology platform; training opportunity; transcriptome sequencing; transcriptomics; whole genome

Project Summary The Research Support Core (RSC) will provide a wide range of high quality quantitative analytical technology platforms spanning metabolomics, epigenomics, transcriptomics, and proteomics. The RSC leader is Dr. Dean P. Edwards, Executive Director of the Advanced Technology Cores at BCM. The RSC co-leader is Dr. Nagireddy Putluri, who also serves as director of the Metabolomics Core and is a recognized leader in mass spectrometry- based metabolomics profiling. The RSC has the following specific aims. AIM 1 Will effectively support the Superfund projects with cutting edge quantitative multi-omics technologies. Established Core technologies available includes mass spectrometry-based targeted and unbiased metabolomics, unbiased proteomic profiling by mass spectrometry, targeted proteomics by antibody-based reverse phase protein array (RPPA) and genomics platforms such as whole genome sequencing, transcriptomics by RNA-seq, smallRNA sequencing and epigenetics by ChIP-Seq. RSC support will include intellectual input from faculty level Core Directors for consultation and experimental design, hosting of advanced instrumentation, executing state-of-the-art analytical procedures by research staff of Cores and processing and analysis of “omics” data sets. RSC technology platforms will be used primarily in Projects 2, 3 and 4 to identify biomarkers and molecular signatures of polycyclic aromatic hydrocarbon (PAH) exposures associated with preterm birth (PTB) and to define the molecular mechanisms underlying the potentiating effects of PAH and its derivatives on chronic lung disease/bronchopulmonary dysplasia (BPD) and neurobehavioral deficits in experimental models and in human studies. The metabolomics core will additionally be instrumental for measuring and quantification of PAH and its metabolites by GC-MS as a standard for development of more sensitive and less sophisticated assays in Projects 1 and 2. AIM 2 Will continuously work with Project leaders and investigators to develop, validate, and deploy novel methods during the course of the grant that are not currently standard Core procedures. This will initially include MS identification of novel PAH derivatives and metabolites, and unbiased metabolomics profiling, epigenetic profiling of histone modifications and chromatin modifying enzymes by RPPA, genome-wide DNA methylation by bisulfite sequencing and Redox proteomics by MS methods. AIM 3 Will train investigators in designing, executing, and interpreting results of the state-of-the-art analytical techniques conducted by the RSC. This will be accomplished by providing tutorials, workshops and some hands-on opportunities for principle investigators of the Superfund grant and their post-doctoral and graduate student trainees. The ultimate goal of the RSC is to provide cutting-edge technical and the best quality scientific solutions available for the Superfund investigators for use in their Aims of understanding PAH exposures at molecular mechanistic and genetic levels.

项目摘要 研究支持核心（RSC）将提供广泛的高定量分析技术 跨越代谢组学，表观基因组学，转录组学和蛋白质组学的平台。 BCM技术核心执行总监P. Edwards。 Putluri也担任代谢组学核心的主管，并且是质谱学领域的公认领导者 - 基于代谢组学分析。 超级基金项目具有尖端定量多摩斯技术技术。 可用的包括基于质谱的靶向和无偏代谢组学，无偏的蛋白质组学分析 通过质谱法，基于抗体的反相蛋白阵列（RPPA）的靶向蛋白质组学和 基因组学平台，例如整个基因组测序，通过RNA-Seq，SmallRNA测序的转录组学 RSC支持的表观遗传学。 咨询和实验设计，主持高级仪器，执行最先进的分析 核心研究人员的程序以及“ OMIC”数据集的处理和分析 平台将主要用于项目2、3和4，以识别多环的生物标志物和分子特征 与早产（PTB）相关的芳族碳氢化合物（PAH）暴露并定义分子 PAH及其衍生物对慢性肺的增强作用的基础机制 实验模型和人类中的疾病/支气管肺发育不良（BPD）和神经行为缺陷 研究。 GC-MS的代谢物作为开发项目中更敏感和更简单的测定的标准 1和2。AIM2将不断与项目负责人和调查人员合作，以开发和部署 在赠款过程中，新的方法是当前不是标准的核心程序。 包括MS鉴定新型PAH衍生物和代谢产物，以及无偏的代谢组学分析， RPPA，全基因组DNA的组蛋白修饰和染色质修饰酶的表观遗传分析 通过亚硫酸盐测序和MS方法通过甲基化和氧化还原蛋白质组学的甲基化。 设计，执行和解释RSC进行的最新分析技术的结果。 这将通过提供教程，讲习班和一些动手原则的机会来访问这一点 超级基金会的调查人员及其博士后和研究生学员的最终目标 RSC的主要是技术和最优质的科学解决方案，可用于超级基金 研究人员用于理解PAH暴露于分子机理和遗传水平的目标。