Wireless optical imaging, optogenetic stimulation and neurophysiological recording to study to neural foundations of natural freely moving behaviors in primates.

无线光学成像、光遗传学刺激和神经生理学记录，用于研究灵长类动物自然自由运动行为的神经基础。

• 批准号: 10260530
• 负责人: Robert Mark Friedman
• 金额: $ 21.22万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10260530
• 关键词: Animals; Area; Attention; Beds; Behavior; Behavioral; Brain; Cells; Cognition; Color; Data; Detection; Development; Devices; Discrimination; Electrophysiology (science); Eye; Foundations; Future; Generations; Goals; Head; Human; Image; Imaging Device; Knowledge; Location; Methods; Monkeys; Motion; Neurons; Neurosciences; Ocular Dominance; Optics; Outcome; Perception; Positioning Attribute; Primates; Production; Regulation; Resolution; Site; System; Technology; Testing; To specify; Training; Visual; Visual Cortex; Visual Perception; Water; Wireless Technology; awake; base; behavioral study; brain machine interface; catalyst; clinically relevant; covert attention; design; electrical microstimulation; gaze; image guided; imaging system; innovation; light weight; multi-electrode arrays; multimodality; neurophysiology; neuroprosthesis; neuroregulation; novel; novel strategies; optical imaging; optogenetics; relating to nervous system; sample fixation; targeted imaging; vector

PROJECT SUMMARY Decades of studies in animals have been conducted in conditions of strict experimental control of perception and behavior in order to understand the functions of the brain. For instance, in the study of visual perception visual paradigms often involve unnaturally long eye fixation periods and attention to locations away from the center of gaze (covert attention). These animal paradigms require long training periods (months to a year). While such studies have advanced our knowledge of brain function tremendously, a recurring question is how relevant the gathered data are to natural behavior. Today, the availability of cutting-edge technologies and computational power can free us from these limitations of the classical experimental paradigms and usher in a new generation of neuroscience questions focused on how the functioning of the brain in real world settings. The goal of this project is to develop a small wireless multimodal device for stimulating and recording domain- based cortical activity in freely moving primates. We will study the function of primary visual cortical areas with intrinsic optical imaging and optogenetic stimulation using a multimodal device that consists of a wireless camera and a wireless multisite LED stimulator. To evaluate the functionality of this approach, animals will perform visual detection and discrimination tasks. The end goal is the production of a multimodal device, constructed from off the shelf components, that provides unrestrained, multiareal and targeted (to specified cortical domains) stimulation and recording capabilities. The successful outcome of this project will contribute to our understanding of cortical encoding of perception and behavior and will have clinical relevance for the development of brain-machine interfaces.

项目摘要 在严格的感知实验控制条件下，已经对动物进行了数十年的研究 和行为以了解大脑的功能。例如，在视觉感知的研究中 视觉范式通常涉及不自然的固定期和对远离地点的关注 目光中心（秘密注意）。这些动物范式需要长时间的训练期（数月到一年）。 尽管此类研究已大大提高了我们对大脑功能的了解，但反复出现的问题是如何 相关收集的数据是自然行为。如今，尖端技术的可用性和 计算能力可以使我们摆脱经典实验范式的这些限制，并引入 新一代的神经科学问题集中在现实世界中大脑的功能上。 该项目的目的是开发一个小型无线多模式设备，用于刺激和记录域 - 自由移动灵长类动物的基于皮质活性。我们将研究主要视觉皮质区域的功能 固有的光学成像和光遗传学刺激使用由无线组成的多模式设备 相机和无线多站点LED刺激器。为了评估这种方法的功能，动物将 执行视觉检测和歧视任务。最终目标是生产多模式设备， 从货架组件中构建的，可提供不受限制的，多拍的和有针对性（指定 皮质域）刺激和记录功能。该项目的成功结果将做出贡献 为了理解对感知和行为的皮质编码的理解，并将与 脑机界面的开发。