ACQUISITION OF A SOLiD 3 SEQUENCER

获取 Solid 3 测序仪

• 批准号: 7796941
• 负责人: Scott Keeney
• 金额: $ 42.5万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2011-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796941
• 关键词: Biological; Biological Models; Cancer Biology; Core Facility; DNA Double Strand Break; DNA Sequence; DNA Sequencing Facility; Data; Detection; Development; Developmental Biology; Drosophila genus; Dyes; Gene Expression Profile; Genetic; Genetic Recombination; Genome; Genomics; Human; Immunology; Institutes; Label; Laboratories; Ligation; Measurement; Memorial Sloan-Kettering Cancer Center; Metagenomics; Methodology; MicroRNAs; Molecular Biology; Mutation Detection; Nucleotides; Oligonucleotides; Pathogenesis; Pattern; Research; Research Personnel; Role; SPO11 gene; Scientist; Signal Transduction; Solid; Techniques; Technology; Time; Variant; anticancer research; base; biological research; epigenomics; flexibility; genome sequencing; instrument; liposarcoma; notch protein; oncology; programs; public health relevance

DESCRIPTION (provided by applicant): The Sloan Kettering Institute requests a SOLiD 3 sequencer. This instrument will be used by scientists to sequence whole or parts of genomes at incredible depth and with extreme accuracy. This instrument will enable various biological applications such as de novo sequencing, epigenomics, mutation detection, transcriptome profiling and metagenomics. The requested instrument will double the sequencing capability of the Sloan Kettering Institute and thereby reduce the turnaround time to 3 to 4 weeks (from 8 weeks currently). The SOLiD 3 methodology is based on sequential ligation with dye-labeled oligonucleotides. This technology advances biological research, particularly cancer research, with its incredibly high accuracy, ultra-high throughput capability and application flexibility. Each nucleotide is interrogated twice during sequencing. This technique reduces measurement errors and produces highly accurate sequence data suitable for the detection of sequence variation, including rare variants. NIH-supported major users include (1) Dr. Scott Keeney, who is studying the role of Spo11 in DNA double-strand breaks that initiate recombination; Dr. Eric Lai, who is using Drosophila as a model system to better understand the effect of Notch signaling and microRNAs on developmental patterning; and Dr. Samuel Singer, who is taking an integrative approach to characterize liposarcoma subtypes molecularly and biochemically. This instrument will also be supporting some more modest projects from investigators affiliated with all the programs in the Sloan Kettering institute: Cancer Biology and Genetics (Joan Massague), Molecular Biology (John Petrini), Immunology (Michael Glickman), Human Oncology and Pathogenesis (Charles Sawyers, David Solit, Ross Levine), and Developmental Biology (Kathryn Anderson, Mary Baylies). The SOLiD 3 sequencer will be located in the Genomics Core Laboratory of MSKCC. This microarray and DNA sequencing core facility has been directed by Dr. Agnes Viale since its inception 8 years ago. Public Health Relevance Statement: The requested instrument will be used by scientists at Memorial Sloan-Kettering Cancer Center to sequence genomes. This acquisition will greatly enhance the pace of these MSKCC research efforts.

描述（由申请人提供）： Sloan Kettering Institute要求一个实体3序列。科学家将使用该仪器以令人难以置信的深度和极高的准确性来对整个基因组进行整体或部分。该仪器将实现各种生物学应用，例如从头测序，表观基因组学，突变检测，转录组分析和宏基因组学。所请求的仪器将使Sloan Kettering Institute的测序能力增加一倍，从而将周转时间减少到3至4周（目前为8周）。实心3方法基于与染料标记的寡核苷酸的顺序连接。这项技术以极高的精度，超高的吞吐量和应用灵活性来推进生物学研究，尤其是癌症研究。在测序过程中，每个核苷酸都会两次询问。该技术减少了测量误差，并产生适合检测序列变化的高度精确序列数据，包括稀有变体。由NIH支持的主要用户包括（1）Scott Keeney博士，他正在研究SPO11在启动重组的DNA双链断裂中的作用；埃里克·莱（Eric Lai）博士，正在使用果蝇作为模型系统，以更好地了解Notch信号传导和microRNA对发育模式的影响；塞缪尔·辛格（Samuel Singer）博士正在采用综合方法来分子和生化。 This instrument will also be supporting some more modest projects from investigators affiliated with all the programs in the Sloan Kettering institute: Cancer Biology and Genetics (Joan Massague), Molecular Biology (John Petrini), Immunology (Michael Glickman), Human Oncology and Pathogenesis (Charles Sawyers, David Solit, Ross Levine), and Developmental Biology (Kathryn Anderson, Mary Baylies). Solid 3 Sequencer将位于MSKCC的基因组核心实验室中。自8年前成立以来，这种微阵列和DNA测序核心设施一直由Agnes Viale博士指导。公共卫生相关性声明：纪念斯隆 - 凯特林癌症中心的科学家将使用所请求的工具来序列基因组。此次收购将大大提高这些MSKCC研究工作的步伐。