Integration of Wolbachia-based Incompatible Insect Technique (IIT) and Auto-Dissemination Augmented by Males (ADAM) for mosquito vector control

基于沃尔巴克氏体的不相容昆虫技术 (IIT) 和雄性自动传播增强技术 (ADAM) 的集成用于蚊媒控制

• 批准号: 10081459
• 负责人: James William Mains
• 金额: $ 19.35万
• 依托单位: MOSQUITOMATE, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10081459
• 关键词: Abate; Adult; Aedes; Affect; Agriculture; American; Area; Award; Bacteria; Behavior; Breeding; Chikungunya virus; Culicidae; Data; Dengue; Diptera; Dust; Female; Fertility; Funding; Genetic; Government; Ingredients and Chemicals; Insecta; Insecticides; Juvenile Hormones; Laboratories; Legal patent; Licensing; Measures; Medical; Methods; Modification; Mosquito Control; Mosquito-borne infectious disease; Performance; Pesticides; Population; Population Replacements; Process; Public Health; Research; Resistance development; Risk; Risk Assessment; Site; Small Business Innovation Research Grant; Sterility; Techniques; Technology; Testing; Therapeutic; United States Environmental Protection Agency; Vaccines; Virus; Wolbachia; Work; ZIKA; base; chikungunya; combat; cost; egg; fitness; improved; male; meetings; microbial; mosquito-borne pathogen; pathogen; prevent; research and development; risk mitigation; sterile insect technique; tool; transmission process; vector control; vector mosquito

Abstract In the absence of effective, approved vaccines or therapeutic measures against dengue, Zika and other mosquito borne pathogens, public health efforts must focus on the control of mosquito vectors that are required for transmission. Unfortunately, effective control measures are not available against invasive mosquito species like Aedes aegypti and Aedes albopictus, due in part to problems resulting from their cryptic behaviour. Specifically, Aedes mosquitoes breed in hidden/inaccessible sites that are sheltered from existing control methods, e.g., truck and aerial spraying. Autocidal technologies use the insect itself as a self-delivering control tool. As an example, the Sterile Insect Technique (SIT) has been used successfully against multiple agricultural pests, including large scale elimination from the North American continent (e.g., Screwworm; Cochliomyia hominivorax). However, despite years of effort, SIT has not proven effective against mosquitoes. Through previous SBIR-funded research, MosquitoMate has developed two autocidal methods against Ae. aegypti and Ae. albopictus mosquitoes, including: 1) Incompatible Insect Technique (IIT) that uses a naturally occurring bacterium (Wolbachia) to sterilize populations, and 2) Auto-Dissemination Augmented by Males (ADAM) that uses males as vehicles to spread a potent insecticide. To date, the USA Environmental Protection Agency (EPA) has instructed MosquitoMate to keep the IIT and ADAM approaches separate. In the work proposed here, MosquitoMate will develop data that the EPA can consider for the integration of the two approaches. If awarded, the resulting research will examine parameters critical to the EPA’s efficacy and risk assessments, including potential: 1) PPF effects on Wolbachia-induced sterility, 2) PPF effects on male mosquito performance (e.g., ‘boosting’ of male competitiveness), and 3) PPF effects on adult female mosquito fecundity and fitness. The resulting data will be communicated to the EPA, including a pre-registration meeting to identify requirements leading to FIFRA Section 3 EPA pesticide registration for the combined IIT/ADAM approach. Market potential exists at the local, corporate, government and area wide control levels for the control of invasive, medically important mosquito species. MosquitoMate has an exclusive license for the patented Wolbachia technology and additional patents on the ADAM technology.

抽象的 在缺乏有效性的情况下，已批准的针对登革热、寨卡病毒的疫苗或治疗措施 和其他蚊媒病原体，公共卫生工作必须集中于控制蚊子 不幸的是，没有有效的控制措施。 可用于对抗埃及伊蚊和白纹伊蚊等入侵蚊种，因为 部分原因是它们的神秘行为造成的问题，特别是伊蚊的繁殖。 隐藏/无法进入的地点，不受现有控制方法（例如卡车和航空）的影响 喷涂。 自杀技术使用昆虫本身作为自我控制工具。 昆虫不育技术（SIT）已成功用于防治多种农业害虫， 包括从北美大陆大规模消灭（例如螺旋虫； 然而，尽管经过多年的努力，SIT 仍未被证明有效。 防蚊。 通过之前 SBIR 资助的研究，MosquitoMate 开发了两种自杀方法 对抗埃及伊蚊和白纹伊蚊，包括： 1) 不相容昆虫技术 (IIT) 使用天然存在的细菌（沃尔巴克氏体）对种群进行绝育，以及 2) 男性增强自动传播（ADAM），利用男性作为传播媒介 迄今为止，美国环境保护署（EPA）已指示使用强效杀虫剂。 MosquitoMate 将 IIT 和 ADAM 方法分开在这里提出的工作中， MosquitoMate 将开发 EPA 可以考虑将两者整合的数据 接近。 如果获奖，由此产生的研究将检查对 EPA 的功效和风险至关重要的参数 评估，包括潜力：1) PPF 对沃尔巴克氏体引起的不育的影响，2) PPF 影响 雄性蚊子的表现（例如，“增强”雄性竞争力），以及 3）PPF 对雄性蚊子的影响 成年雌性蚊子的繁殖力和健康状况的数据将被传达给。 EPA，包括预注册会议，以确定 FIFRA 第 3 节的要求 EPA 农药登记采用 IIT/ADAM 组合方法。 地方、企业、政府和地区范围内的控制层面存在市场潜力 MosquitoMate 具有独特的功能，可以控制具有重要医学意义的入侵性蚊子种类。 Wolbachia 专利技术和 ADAM 技术附加专利的许可。