TISSUE MODELING CORE

组织建模核心

• 批准号: 10006218
• 负责人: PAUL OKUNIEFF
• 金额: $ 21.49万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-17 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10006218
• 关键词: Address; Archives; Area; Basic Science; Benign; Biocompatible Materials; California; Cancer Burden; Cancer Center; Cell Culture Techniques; Cell Line; Clinical; Clinical Research; Collaborations; Collection; Complex; Comprehensive Cancer Center; Conscious; County; DNA; Data; Development; Disease; Education; Ensure; Epidemiology; Evaluation; Florida; Funding; Future; Genetic; Genomic DNA; Goals; Growth; Health Services Accessibility; Human; In Vitro; Infrastructure; Institutes; Institution; International; Latino; Life Style; Link; Los Angeles; Malignant neoplasm of pancreas; Malignant neoplasm of prostate; Medical Research; Medical center; Mission; Mitochondria; Normal tissue morphology; Oncologist; Outcome; Pancreas; Pancreatectomy; Pathologic; Patients; Pharmacologic Substance; Pharmacy facility; Pilot Projects; Plasma; Population; Procedures; Process; RNA; Race; Radiation Oncology; Research; Research Personnel; Residual state; Resources; Sampling; Science; Services; Specimen; Technology; Testing; Therapeutic; Tissue Banks; Tissue Model; Tissue Procurements; Tissue Sample; Tissues; Toxicology; Transference; Translational Research; Tumor Tissue; Tumor-Derived; Universities; Xenograft procedure; anticancer research; base; biobank; blood product; cancer health disparity; cancer type; clinical database; college; comorbidity; drug discovery; ethnic diversity; experience; gemcitabine; genomic data; health equity; improved; mid-career faculty; neoplasm registry; neoplasm resource; pancreatic cancer patients; pancreatic neoplasm; patient oriented; patient population; population based; racial diversity; recruit; repository; response; skills; success; three-dimensional modeling; transcriptome sequencing; transcriptomics; tumor; virtual

PROJECT ABSTRACT: TISSUE MODELING CORE The long-term goal of the Tissue Modeling Core (TMC) is to provide a growing archive of fixed and living tissues, DNA, and RNA for sequencing that will support the Florida-California CaRE2 Health Equity Center’s mission. The TMC will develop a robust, well-annotated biorepository of tissue samples from a racially and ethnically diverse Florida and California patient population and make them available to the CaRE2 Center investigators. The short-term goals of the TMC are to provide the necessary tissue samples for the three projects while preparing for the future needs of the existing and upcoming CaRE2 projects. The TMC will address the challenge of recruiting Blacks and Latinos in medical research and the limited supply of quality biospecimens and genomic data for these populations. The TMC Specific Aims are to: Aim 1. Develop a virtual repository that will provide access to biospecimens from diverse tumor repositories through a single access point provided by the CaRE2 Center. Many cancer types have been linked to outcome disparities, with the causes of disparities including genetics, access to care, comorbidities, and lifestyle. Aim 1A: Tissue and biomaterials collection. The established tissue-modeling repository will comprise: (1) the UF CTSI tissue repositories; and (2) the USC Residual Tissue Repository linked to the California Cancer Registry and Norris Comprehensive Cancer Center/Los Angeles County Medical Center patient samples. The immediate tasks of the TMC include: (1) Full Project 1: provision of tissue, plasma, serum, and RNA needed for evaluation of mitochondrial peptidomics and transcriptomics of prostate cancer; (2) Full Project 2: provision of tissue and RNA of pancreatic cancers to understand gemcitabine efficacy in pancreatic cancer; and (3) Pilot Project 3: provision of normal pancreas tissues and sequencing for understanding pancreatic cancer development. Aim 1B: Infrastructure transfer to FAMU. The TMC will also seek to expand the FAMU infrastructure for fixed and living tissues. It will leverage that growth using the cooperating CTSIs, Cancer Centers, and the OneFlorida Clinical Research Consortium. Aim 2. Living tissue and living tumor tissue acquisition. The immediate tasks of the TMC include: (1) Full Project 2: provision of pancreatic cancer PDXs and paired cell lines for 2D and 3D modeling; and (2) Pilot Project 3: provision of normal living pancreas tissues from patients undergoing pancreatic surgery for benign disease. By achieving these Specific Aims, the TMC will: (1) enhance cancer disparities science in the important areas of tissue-modeling research; (2) efficiently expand cancer disparities resources for CaRE2 researchers by leveraging the expertise, services, and facilities in Florida and California, and (3) ensure a sustained focus on transferring know-how, technology, and collaborations with FAMU.

项目摘要：组织建模核心 组织建模核心（TMC）的长期目标是提供日益增长的固定和生活档案 将支持佛罗里达 - 加利福尼亚Care2健康权益中心的组织，DNA和RNA进行测序 使命。 TMC将从种族和 种族多样化的佛罗里达州和加利福尼亚州的患者人口，并将其用于Care2 Center 调查人员。 TMC的短期目标是为这三个项目提供必要的组织样本 在为现有和即将到来的CARE2项目的未来需求做准备时。 TMC将解决 在医学研究中招募黑人和拉丁美洲人的挑战以及质量生物测量的有限供应 这些人群的基因组数据。 TMC的特定目的是： 目标1。开发一个虚拟存储库，该存储库将提供从潜水员肿瘤的生物测量访问 通过CARE2中心提供的单个访问点存储库。许多癌症类型已经 与结果差异有关，以及差异的原因，包括遗传学，获得护理，合并症， 和生活方式。 AIM 1A：组织和生物材料收集。已建立的组织模型存储库将 包括：（1）UF CTSI组织存储库； （2）USC残留组织存储库与 加利福尼亚癌症注册表和诺里斯综合癌症中心/洛杉矶县医疗中心 病人样本。 TMC的直接任务包括：（1）完整项目1：提供组织，血浆，血清， 以及评估前列腺癌的线粒体胡椒和转录组学所需的RNA； （2）满 项目2：提供胰腺癌的组织和RNA，以了解胰腺的吉西他滨效率 癌症; （3）试点项目3：提供正常胰腺组织和测序以理解 胰腺癌发展。 AIM 1B：基础设施转移到FAMU。 TMC也将寻求扩展 固定组织和活组织的FAMU基础设施。它将利用合作CTSI的增长， 癌症中心和Oneflorida临床研究联盟。 目标2。活组织和活肿瘤组织的获取。 TMC的直接任务包括： （1）完整项目2：提供胰腺癌PDX和配对细胞系，用于2D和3D建模； （2） 试点项目3：从接受胰腺手术的患者提供正常生活胰腺组织 良性疾病。 通过实现这些特定目标，TMC将：（1）增强重要的癌症分布科学 组织模型研究的领域； （2）通过 利用佛罗里达和加利福尼亚的专业知识，服务和设施，（3）确保持续关注 转移知识，技术和与FAMU的合作。