Metabolomics Shared Resource

代谢组学共享资源

基本信息

批准号：
10025013
负责人：
Nagireddy Putluri
金额：
$ 9.31万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10025013
关键词：
Biological Biological Markers Biological Models Cancer Biology Cancer Center Cancer Center Support Grant Cancer Model Cancer Research Project Carbon Cell Line Cells Citric Acid Cycle Clinical Column Chromatography Communication Comprehensive Cancer Center Computing Methodologies Consultations Custom Data Data Analyses Data Analytics Databases Development Disease Early Diagnosis Emerging Technologies Experimental Designs Experimental Models Folic Acid Generations Genetic Glucose Glutaminase Glutamine Glycolysis Goals Grant In Vitro Infrastructure Journals Label Lipids Liquid Chromatography Liquid substance Malignant Neoplasms Mass Spectrum Analysis Measurement Measures Medicine Metabolic Metabolic Marker Metabolic Pathway Metabolism Methionine Methods Mission Molecular Monitor Multiomic Data Mus NCI Center for Cancer Research Nature Pathologic Pathway interactions Pentosephosphate Pathway Physiological Play Positioning Attribute Proteins Publications Publishing Reaction Reporting Research Research Personnel Resource Sharing Role Sampling Science Services Shotguns Specimen Stable Isotope Labeling Standardization System Technology Therapeutic Time Tissues Translating Tryptophan Tumor-Derived Work anticancer research base biomarker panel cancer cell clinical investigation clinical translation college experience fatty acid biosynthesis in vivo in vivo Model innovative technologies insight instrument instrumentation interest liquid chromatography mass spectrometry mass spectrometer member metabolic profile metabolome metabolomics metabolomics resource nucleotide metabolism nutrition operation outcome forecast oxidation programs small molecule triple-negative invasive breast carcinoma tumor tumor progression

项目摘要

PROJECT SUMMARY: Metabolomics Shared Resource (MSR) The Metabolomics Shared Resource (MSR) is evolving to fulfill the research needs and mission of the Dan L Duncan Comprehensive Cancer Center (DLDCCC). This Shared Resource (SR) provides advanced mass spectrometry approaches and technologies, scientific consultation, and expert data analytics as is required for high quality global and targeted metabolomics studies. The MSR specializes in discovery, identification, characterization, and quantification of biomolecules, as well as metabolic flux analysis, from a variety of biological specimens including tissues, cell lines, and fluids. The main infrastructure of the MSR consists of 5 high-end mass spectrometry systems and upfront liquid chromatography columns that are most suitable for covering the range of metabolomic analyses developed as services including an Agilent 6490 Triple Quad Mass Spectrometer, 2 Agilent 6495 Triple Quad Mass Spectrometers, an Agilent 6550 Time of Flight Mass Spectrometer, and a SCIEX Triple TOF mass spectrometer with Schimadzu Nexera UHPLC-System with data- independent acquisition for lipidomics. The MSR has a dedicated team of experts in mass spectrometry with extensive experience in metabolomic profiling and biological expertise in cellular metabolism and data analysis. Metabolomics became a Shared Resource of the Cancer Center for the first time at the last competing renewal of the CCSG in 2015. Since then, the MSR has supported a broad range of DLDCCC members across 6 of the 7 Programs and a total of 57 publications, many representing major projects in high- impact journals including Nature, Journal of Clinical Investigations, Nature Communications, PLoS Genetics, Nature Metabolism, Cell Reports, Cancer Cell, Cancer Research, and Clinical Cancer Research. This is a dynamic SR facility that has kept pace with rapidly emerging technologies and instrumentation and has been a leader in the field by developing and publishing innovative technologies. The MSR has developed state-of-the- art technologies as core support services that includes the following: (1) Steady-state targeted metabolomics with a capacity for quantification of up to 700 known metabolites; (2) Untargeted metabolomics for identification of up to 1200 metabolites; (3) Lipidomics for up to 800 molecules and; (4) Metabolic flux analysis to monitor changes in activities of major metabolic pathways in experimental model systems. The MSR, as proposed in this application, can bridge the gap in our understanding of molecular pathways involved in cancer biology and enable a wide range of DLDCCC research groups to explore the role of metabolism in cancer utilizing cutting- edge technologies with quality, efficiency, and accuracy which would not be possible otherwise.

项目摘要：代谢组学共享资源 (MSR) 代谢组学共享资源 (MSR) 正在不断发展，以满足 Dan L 的研究需求和使命邓肯综合癌症中心 (DLDCCC)。该共享资源 (SR) 提供先进的质量光谱测定方法和技术、科学咨询以及专家数据分析高质量的全球和有针对性的代谢组学研究。 MSR 专门从事发现、识别、各种生物分子的表征和定量以及代谢通量分析生物样本，包括组织、细胞系和液体。 MSR的主要基础设施包括5个最适合的高端质谱系统和前期液相色谱柱涵盖作为服务开发的代谢组学分析范围，包括 Agilent 6490 三重四极杆质谱仪，2 个 Agilent 6495 三重四极杆质谱仪，1 个 Agilent 6550 飞行时间质量光谱仪和配备 Schimadzu Nexera UHPLC 系统的 SCIEX Triple TOF 质谱仪，具有数据- 脂质组学的独立采集。 MSR 拥有专门的质谱专家团队在代谢组学分析方面拥有丰富的经验以及细胞代谢和数据方面的生物学专业知识分析。代谢组学终于首次成为癌症中心的共享资源 2015 年 CCSG 的竞争更新。从那时起，MSR 支持了广泛的 DLDCCC 7 个计划中 6 个计划的成员以及总共 57 份出版物，其中许多代表了高水平领域的重大项目影响力期刊，包括 Nature、Journal of Clinical Investigations、Nature Communications、PLoS Genetics、自然代谢、细胞报告、癌细胞、癌症研究和临床癌症研究。这是一个动态 SR 设施与快速新兴的技术和仪器保持同步，并已成为通过开发和发布创新技术成为该领域的领导者。 MSR 已开发出最先进的艺术技术作为核心支持服务，包括以下内容：（1）稳态靶向代谢组学能够定量多达 700 种已知代谢物； (2)非靶向代谢组学鉴定多达 1200 种代谢物； (3) 多达 800 个分子的脂质组学； (4) 代谢通量分析监测实验模型系统中主要代谢途径活性的变化。 MSR，如所提议的该应用程序可以弥合我们对癌症生物学和分子途径的理解之间的差距使广泛的 DLDCCC 研究小组能够利用切割来探索代谢在癌症中的作用具有质量、效率和准确性的尖端技术，这是其他方式不可能实现的。