Metabolomics Shared Resource

代谢组学共享资源

• 批准号: 10674555
• 负责人: Nagireddy Putluri
• 金额: $ 9.31万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674555
• 关键词: Biological; Biological Markers; Biological Models; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cancer Model; Cancer Research Project; Carbon; Cell Line; Cells; Citric Acid Cycle; Clinical; Column Chromatography; Communication; Comprehensive Cancer Center; Computing Methodologies; Consultations; Data; Data Analyses; Data Analytics; Databases; Dedications; Development; Disease; Early Diagnosis; Emerging Technologies; Experimental Designs; Experimental Models; Folic Acid; Generations; Genetic; Glucose; Glutaminase; Glutamine; Glycolysis; Goals; Grant; In Vitro; Infrastructure; Journals; Label; Lipids; Liquid Chromatography; Liquid substance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Medicine; Metabolic; Metabolic Marker; Metabolic Pathway; Metabolism; Methionine; Methods; Mission; Molecular; Monitor; Multiomic Data; Mus; NCI Center for Cancer Research; Nature; Pathologic; Pathway interactions; Pentosephosphate Pathway; Physiological; Play; Positioning Attribute; Prognosis; Proteins; Publications; Publishing; Reaction; Reporting; Research; Research Personnel; Resource Sharing; Role; Sampling; Science; Services; Shotguns; Specimen; Stable Isotope Labeling; Standardization; System; Technology; Therapeutic; Time; Tissues; Translating; Tryptophan; Tumor-Derived; Work; anticancer research; biomarker panel; cancer cell; candidate identification; clinical investigation; clinical translation; college; experience; fatty acid biosynthesis; in vivo; in vivo Model; innovative technologies; insight; instrument; instrumentation; interest; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; member; metabolic profile; metabolome; metabolomics; metabolomics resource; nucleotide metabolism; nutrition; operation; oxidation; programs; small molecule; technology platform; triple-negative invasive breast carcinoma; tumor; tumor progression

PROJECT SUMMARY: Metabolomics Shared Resource (MSR) The Metabolomics Shared Resource (MSR) is evolving to fulfill the research needs and mission of the Dan L Duncan Comprehensive Cancer Center (DLDCCC). This Shared Resource (SR) provides advanced mass spectrometry approaches and technologies, scientific consultation, and expert data analytics as is required for high quality global and targeted metabolomics studies. The MSR specializes in discovery, identification, characterization, and quantification of biomolecules, as well as metabolic flux analysis, from a variety of biological specimens including tissues, cell lines, and fluids. The main infrastructure of the MSR consists of 5 high-end mass spectrometry systems and upfront liquid chromatography columns that are most suitable for covering the range of metabolomic analyses developed as services including an Agilent 6490 Triple Quad Mass Spectrometer, 2 Agilent 6495 Triple Quad Mass Spectrometers, an Agilent 6550 Time of Flight Mass Spectrometer, and a SCIEX Triple TOF mass spectrometer with Schimadzu Nexera UHPLC-System with data- independent acquisition for lipidomics. The MSR has a dedicated team of experts in mass spectrometry with extensive experience in metabolomic profiling and biological expertise in cellular metabolism and data analysis. Metabolomics became a Shared Resource of the Cancer Center for the first time at the last competing renewal of the CCSG in 2015. Since then, the MSR has supported a broad range of DLDCCC members across 6 of the 7 Programs and a total of 57 publications, many representing major projects in high- impact journals including Nature, Journal of Clinical Investigations, Nature Communications, PLoS Genetics, Nature Metabolism, Cell Reports, Cancer Cell, Cancer Research, and Clinical Cancer Research. This is a dynamic SR facility that has kept pace with rapidly emerging technologies and instrumentation and has been a leader in the field by developing and publishing innovative technologies. The MSR has developed state-of-the- art technologies as core support services that includes the following: (1) Steady-state targeted metabolomics with a capacity for quantification of up to 700 known metabolites; (2) Untargeted metabolomics for identification of up to 1200 metabolites; (3) Lipidomics for up to 800 molecules and; (4) Metabolic flux analysis to monitor changes in activities of major metabolic pathways in experimental model systems. The MSR, as proposed in this application, can bridge the gap in our understanding of molecular pathways involved in cancer biology and enable a wide range of DLDCCC research groups to explore the role of metabolism in cancer utilizing cutting- edge technologies with quality, efficiency, and accuracy which would not be possible otherwise.

项目摘要：代谢组学共享资源（MSR） 代谢组学共享资源（MSR）正在发展，以满足Dan L的研究需求和使命 邓肯综合癌症中心（DLDCCC）。此共享资源（SR）提供高级质量 光谱方法和技术，科学咨询和专家数据分析 高质量的全球和靶向代谢组学研究。 MSR专门研究发现，身份证明， 来自多种生物分子的表征和定量生物分子以及代谢通量分析。 包括组织，细胞系和液体在内的生物标本。 MSR的主要基础设施由5组成 高端质谱系统和最适合的前期液相色谱柱 涵盖作为服务的代谢组分析范围，包括Agilent 6490三倍四Quad 质谱仪，2个Agilent 6495三倍四倍质量光谱仪，一个安捷伦的6550飞行时间 光谱仪和带有数据的Schimadzu Nexera UHPLC系统的Sciex Triple ToF质谱仪 独立获取脂质组学。 MSR拥有一支专门的质谱专家团队 在代谢组分析和细胞代谢和数据方面的生物学专业知识方面的丰富经验 分析。代谢组学首次成为癌症中心的共享资源 2015年CCSG的竞争续约。从那以后，MSR支持广泛的DLDCCC 在这7个计划中有6个和总共57个出版物中的成员，许多人代表高级项目的主要项目 影响包括自然在内的影响期刊，临床调查杂志，自然通讯，PLOS遗传学， 自然代谢，细胞报告，癌细胞，癌症研究和临床癌症研究。这是一个 动态的SR设施已与快速新兴的技术和仪器保持同步，并且一直是 该领域的领导者通过开发和发布创新技术。 MSR已开发出最新的 艺术技术作为核心支持服务，其中包括以下内容：（1）稳态靶向代谢组学 具有多达700个已知代谢产物的能力； （2）识别的非靶向代谢组学 多达1200个代谢产物； （3）多达800个分子的脂质组学和； （4）代谢通量分析以监测 实验模型系统中主要代谢途径活动的活动变化。 MSR，如提议 该应用程序可以弥合我们对癌症生物学涉及的分子途径的理解和 使各种DLDCCC研究小组能够探索新陈代谢在利用过滤的癌症中的作用 - 具有质量，效率和准确性的边缘技术是不可能的。