In Vitro Models and Cell Culture Core

体外模型和细胞培养核心

• 批准号: 10024663
• 负责人: Joseph Zabner
• 金额: $ 18.54万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10024663
• 关键词: Biological Models; Cell Culture Techniques; Cell Line; Cells; Collaborations; Cultured Cells; Cystic Fibrosis; Data; Development; Electrophysiology (science); Epithelial; Epithelial Cells; Epithelium; Evaluation; Expression Profiling; Family suidae; Ferrets; Fibroblasts; Flow Cytometry; Functional disorder; Gene Expression; Generations; Goals; Health; Human; In Vitro; Institution; Iowa; Methodology; Methods; Mission; Modeling; Molecular; Morphology; Mutation; Phenotype; Process; Production; Pulmonary Cystic Fibrosis; Records; Research; Research Personnel; Resources; Services; Study models; Technical Expertise; Tissues; Universities; airway epithelium; biobank; bioelectricity; cell type; cystic fibrosis airway; experimental study; gene therapy; human model; human tissue; in vitro Model; investigator training; tissue resource

PROJECT SUMMARY/ABSTRACT - In Vitro Models Cell Culture Core (CORE B ) The In Vitro Models and Cell Culture Core was established to provide well-differentiated epithelial models for studies on the pathophysiology of cystic fibrosis (CF) and for molecular and gene therapy experiments. The In Vitro Models and Cell Culture Core mission is to advance CF research to impact the health of people with CF. The Core achieves that mission by providing relevant models and technical expertise to investigators, developing new models and approaches, training investigators, and attracting new investigators to CF research and aiding their development. The goals of the In Vitro Models and Cell Culture Core are to collect, process and biobank tissue and airway epithelia from human, pig, and ferret. The Core also maintains a biobank of human non-CF and CF epithelia, including CF epithelia with rare mutations. The Core will also characterize models and native epithelia in collaboration with other Cores and investigators and will perform bioelectric analyses, morphological evaluation, gene expression profiles, and flow cytometry. Finally, the Core will teach investigators at the University of Iowa and other institutions how to successfully implement the methods for in vitro model systems.

项目摘要/摘要 - 体外模型细胞培养核心（核心B） 建立了体外模型和细胞培养核心，以提供明显的上皮模型 囊性纤维化（CF）的病理生理学以及分子和基因治疗实验的研究。 in 体外模型和细胞培养核心使命是进步CF研究，以影响CF患者的健康。 核心通过向调查人员提供相关模型和技术专长来实现这一任务，开发 新的模型和方法，培训调查人员，并吸引新的研究人员进行CF研究和帮助 他们的发展。体外模型和细胞培养核心的目标是收集，过程和生物库 来自人，猪和雪貂的组织和气道上皮。核心还保持人类非CF的生物库 和CF上皮，包括具有罕见突变的CF上皮。核心还将表征模型和本地 上皮与其他核心和研究人员合作，将进行生物电分析， 形态评估，基因表达谱和流式细胞仪。最后，核心将教导 爱荷华大学和其他机构的调查人员如何成功实施IN的方法 体外模型系统。