Model Systems Core

模型系统核心

基本信息

批准号：
10747626
负责人：
AROHAN R SUBRAMANYA
金额：
$ 29.59万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747626
关键词：
Acceleration Acids Animal Model Animals Applications Grants Bioenergetics Biological Models Blinded CRISPR/Cas technology Cardiovascular system Catalogs Cell Culture Techniques Cell Line Cell Separation Cell Volumes Cells Collaborations Communities Complex Computer Models Creativeness Data Data Set Development Disease Disease model Distal Ensure Environment Epithelium Evaluation Experimental Designs Feedback Functional disorder Generations Genetic study Genotype Goals Grant Health Homeostasis Hormone Responsive Human Individual Infrastructure Instruction Investigation Journals Kidney Kidney Diseases Leadership Liquid substance Measures Metabolism Methods Mitochondria Modeling Monitor Mus Nephrons Organism Organoids Output Peer Review Pharmaceutical Preparations Physiology Play Process Productivity Protocols documentation Publications Purines Quality Control Reagent Recording of previous events Renal function Reproducibility Research Research Personnel Resolution Resource Development Resource Sharing Resources Rodent Rodent Model Role Saccharomyces cerevisiae Scientist Services Specialized Center System Technology Testing Training Validation Work Yeast Model System Yeasts absorption base biomedical resource cell immortalization cell type design experimental study gene conservation hands on instruction induced pluripotent stem cell innovation kidney cell meetings member metabolomics nephrogenesis new technology next generation proteostasis recruit response to injury sabbatical skills social media solute tool web site

项目摘要

The Model Systems Core continues the Pittsburgh Center for Kidney Research’s rich history of using creative approaches to drive the latest conceptual advances in kidney physiology and pathophysiology. The goal of this biomedical resource Core is to provide a diverse array of innovative model systems that will allow kidney researchers to explore renal development, function, and systemic physiology in normal and disease states. The Core is divided into three Subcores that extend from simple unicellular organisms to complex whole animal models. Taking advantage of the genetically tractable organism S. cerevisiae, the Yeast Models Subcore provides a toolbox for the analysis of conserved genes that play essential roles in kidney physiology and proteostasis. The Epithelial Models Subcore provides access to high fidelity immortalized proximal and distal tubule cell lines, primary cell isolations, and state-of-the art human kidney organoids for the development of disease models, analysis of nephron development, metabolism, function, and response to injury. The Animal Models Subcore provides a broad repertoire of methods to evaluate rodent kidney physiology and pathophysiology, associated cardiovascular variables, mitochondrial health and bioenergetics, and purine metabolomics. Using an infrastructure supported by its established investigators and record of productivity, the Model Systems Core will work seamlessly with the Physiology Core and the KIDNIT Resource Development Core to facilitate discovery. As part of the Consortium, the Core will ensure a facile exchange of tools, technologies, and specialized Center resources to advance kidney research nationwide. Accordingly, the Core welcomes all collaborators, offers mechanisms to promote Subcore tools and reagents, provides hands-on instruction (i.e., mini-sabbaticals), and will facilitate collaborative resource sharing with individual investigators as well as with the Consortium at large. For early-stage investigators and established PIs who are new to kidney research, key resources, training, and collaborative opportunities will be prioritized to cultivate enthusiasm and entry into an active research pipeline. The Core will also provide expert advice and aid in the acquisition of preliminary data to support new and innovative grant applications. Stringent quality control measures will ensure the rigor and reproducibility of Subcore outputs, and a formalized evaluation and feedback process permits regular reassessment of the usefulness of Core resources. By offering impactful state-of-the-art and readily accessible services across diverse model systems, the Core will provide a unique national resource for the kidney research community that will propel team-based investigation and catalyze discovery.

模型系统核心继续匹兹堡肾脏研究中心的丰富历史记录推动肾脏生理学和病理生理学最新概念进步的方法。目标的目标生物医学资源核心是提供一系列创新模型系统，这些系统将允许肾脏研究人员探索正常和疾病状态下的肾脏发育，功能和系统生理。核心分为三个子核，从简单的单细胞生物延伸到复杂的全动物型号。利用一般可牵引的生物S. cerevisiae，酵母模型子核提供了一个工具箱，用于分析组成基因，该基因在肾脏生理学和蛋白毒酸。上皮模型子核可访问高富达永生的近端和不同小管细胞系，主要细胞隔离和最先进的人类肾脏器官，以开发疾病模型，肾脏发育的分析，代谢，功能和对损伤的反应。动物模型子核提供了广泛的方法来评估啮齿动物肾脏生理学和病理生理学，相关心血管变量，线粒体健康和生物能学以及嘌呤代谢组学。使用其既定研究人员支持的基础设施和生产力的记录，模型系统核心将与生理学核心和绑架资源开发无缝合作促进发现的核心。作为财团的一部分，核心将确保工具的便捷交换，技术和专门的中心资源，以推进全国肾脏研究。根据核心欢迎所有合作者，提供促进子核工具和试剂的机制，并提供动手指导（即迷你邮编），并将促进与个人调查员的合作资源共享以及整个财团。适用于肾脏新手的早期调查人员和已建立的PI 研究，关键资源，培训和协作机会将优先考虑，以培养热情和进入主动研究管道。核心还将提供专家建议并帮助收购初步数据以支持新的和创新的赠款应用程序。严格的质量控制措施将确保子核心产出的严格和可重复性以及正式的评估和反馈过程允许定期重新评估核心资源的实用性。通过提供有影响力的最先进并随时提供跨潜水员模型系统可访问的服务，核心将为该公司提供独特的国家资源肾脏研究社区将推动基于团队的投资并催化发现。