Cell Culture Core

细胞培养核心

• 批准号: 7741487
• 负责人: Joseph Zabner
• 金额: $ 28.86万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7741487
• 关键词: Animals; Bacteria; Biological Models; Bronchoalveolar Lavage; Cell Culture Techniques; Cell Line; Cell model; Cells; Commit; Comparative Study; Conditioned Culture Media; Conflict (Psychology); Cultured Cells; Development; Educational process of instructing; Epithelium; Evaluation; Family suidae; Fibroblasts; Gene Transfer; Generations; Gland; Goals; Human; Human Resources; Individual; Institution; Laboratories; Medical Research; Methods; Microbiology; Modeling; Phenotype; Plasmids; Process; Production; Pulmonary Cystic Fibrosis; Quality Control; Research Design; Research Personnel; Resources; Structure; Study models; Suggestion; Time; Tissues; Universities; Viral; Virus; Work; airway epithelium; cell type; density; experience; gene therapy; improved; in vitro Model; innovation; insight; research and development; research study; square foot; trafficking

The Core is structured so that all the work is performed by Core personnel. Individual investigators who wish to use cells or other materials or to develop new models discuss the type of cells needed, the culture conditions, and the overall goal of the experiment or project with Core personnel. Then, Core personnel provide the cells and models to the investigator. Frequently, the individual investigator and Core personnel work together to provide the optimal conditions (media, seeding density, duration of incubation, substrates, passage number, etc) required for a specific type of study. The development of new models is usually an iterative process, with investigators talking to the Core, Core personnel developing a model, the investigator evaluating it, and making suggestions, the Core personnel attempting to improve the model, and so on. The organization of the Core has several advantages, a) It provides the highest level of quality control. We rarely have infected cell lines and alterations in phenotype are detected eariy. b) It provides the best use of facilities, c) By having Core personnel do all the work of the Core, traffic and time conflicts are minimized, d) It provides PPG investigators with a highly experienced and innovative staff who are committed to developing models that will allow investigators to generate new insight into CF. The Core is located in approximately 550 square feet of space in rooms 567, 571, and 500B of the Eckstein Medical Research Building (EMRB). EMRB contains the laboratories of all 4 project Pis (Drs. Davidson, Welsh, McCray, and Zabner).

核心是结构化的，因此所有工作都是由核心人员执行的。个人调查员 希望使用细胞或其他材料或开发新模型的人讨论所需的细胞类型，培养条件以及实验或核心人员项目的总体目标。然后，核心人员向研究人员提供细胞和模型。通常，单个研究者和核心人员共同努力，以提供特定类型的研究所需的最佳条件（培养基，播种密度，孵育持续时间，底物，通道数等）。新模型的开发通常是一个迭代过程，研究人员与核心人员交谈，核心人员开发模型，研究人员对其进行评估，并提出建议，试图改善模型的核心人员等等。 核心的组织具有多个优势，a）它提供了最高质量的水平 控制。我们很少感染细胞系，并且检测到表型的改变。 b）它提供了最佳利用设施，c）让核心人员完成核心的所有工作，交通和时间冲突的最小化，d）为PPG调查人员提供经验丰富且创新的员工，他们致力于开发模型，以允许研究人员对CF产生新的见解。 核心位于567、571和500B的房间中约550平方英尺的空间中 埃克斯坦医学研究大楼（EMRB）。 EMRB包含所有4个PIS项目的实验室（Davidson，Welsh，McCray和Zabner博士）。