Functional Genomics of Dictyostelium

盘基网柄菌的功能基因组学

• 批准号: 7286778
• 负责人: ADAM KUSPA
• 金额: $ 87.17万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-19 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286778
• 关键词: Functional; Genomics; Dictyostelium

DESCRIPTION (provided by applicant): The long-term objectives of this program project are to define the cellular regulatory mechanisms that govern cell differentiation in eukaryotes using Dictyostelium discoideum as a model. This system can be used to provide a complete picture of the regulation of a significant biological problem: the integration of individual cells into a multicellular tissue with the proper form and function. Several specific cellular systems and regulatory pathways will be highlighted in these studies which may illuminate regulatory systems that are fundamental to all eukaryotes. The function of these signaling pathways in Dictyostelium will be studied by genetic, physiological, and genomic methods. As new protein components of these pathways are uncovered, their functions will be determined, and the relationships among them explored, by examining mutant phenotypes, transcriptional profiles, and other physiological measures. Transcription levels of most genes in the genome will be measured for all mutants studied and for wild-type cells under various conditions. The pattern of gene expression will be interpreted in two ways. First, function will be assigned to genes based on their transcriptional regulation, according to the notion that a gene is expressed when and where it is needed. Second, the pattern of expression of all the genes will be determined for all the mutant strains, and the investigators will attempt to assign function to the mutated genes based on this transcriptional phenotype. Describing complex biological pathways is the result of integrating information on many individual components and on their interactions. This is most easily done in relatively simple systems that afford the use of powerful molecular tools, such as Dictyostelium. These descriptions will likely impact our understanding of, and ability to treat, human disease.

描述（由申请人提供）：该计划项目的长期目标是使用盘基网柄菌作为模型来定义控制真核细胞分化的细胞调节机制。 该系统可用于提供重要生物学问题调节的完整图像：将单个细胞整合为具有适当形式和功能的多细胞组织。 这些研究将重点介绍几个特定的​​细胞系统和调控途径，这些研究可能会阐明对所有真核生物至关重要的调控系统。 将通过遗传、生理和基因组方法研究盘基网柄菌中这些信号通路的功能。 随着这些途径的新蛋白质成分的被发现，它们的功能将被确定，并通过检查突变表型、转录谱和其他生理测量来探索它们之间的关系。 将在各种条件下测量所有研究的突变体和野生型细胞的基因组中大多数基因的转录水平。 基因表达模式可以用两种方式解释。 首先，根据基因在需要的时间和地点表达的概念，根据基因的转录调控将功能分配给基因。 其次，将确定所有突变菌株的所有基因的表达模式，研究人员将尝试根据这种转录表型将功能分配给突变基因。 描述复杂的生物途径是整合许多单独成分及其相互作用的信息的结果。 这在相对简单的系统中最容易完成，这些系统可以使用强大的分子工具，例如盘基网柄菌。 这些描述可能会影响我们对人类疾病的理解和治疗能力。