FUNCTIONAL GENOMICS OF DICTYOSTELIUM

盘基网菌的功能基因组学

• 批准号: 6892873
• 负责人: ADAM KUSPA
• 金额: $ 115.53万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-19 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6892873
• 关键词: Dictyostelium; functional /structural genomics

DESCRIPTION: The availability of complete eukaryotic genome sequences promises to accelerate research into fundamental mechanisms of cell and developmental biology. Before one can fully exploit this information and study the biology of an organism from the context of its entire genome one must be able to: (1) identify the relevant information within the genome, (2) understand how the information is translated into function, and (3) integrate these data into a comprehensive biological picture. The investigators' are developing tools, both experimental and theoretical, that will facilitate these goals for the study of all organisms. They intend to test these tools by analyzing the soil amoebae Dictyostelium discoideum, and by comparing there findings to those found in the analyses of other genomes. The project will combine technology development and genetic resource acquisition with genome-based approaches to functional analysis. Recent published reports provide ample evidence that microarray data can be used effectively for genetic analysis (phenotyping, epistasis tests, etc.). The investigators' basic idea is that the transcriptional state of a cell can be used as a high-resolution phenotype with the necessary discriminating power to place all of the genes in Diclyostelium within a framework regulatory network, from which, the appropriate experiments to test molecular mechanisms can be planned. The investigators' will examine the expression of all genes during the growth and development of Diclyostelium within each of about 5,000 knockout mutants. This data, when combined with the fitness data from parallel phenotyping and other traditional phenotyping, will allow us to make testable predictions of gene function and to propose regulatory networks. The unique value of functional genomics lies in the power of large databases to provide a broad, and presumably less-biased, view of biological systems. This approach will open the exploration of development to a broader viewpoint. It will re-emphasize the entire organism as the fundamental biological unit and reveal relationships between signaling pathways and cell-types that could not be appreciated from the analyses of small groups of genes. The investigators' are interested in those aspects of Dictyostelium biology that are common to all eukaryotic organisms, and that will be informative for defining both the function of individual genes and the organization of regulatory hierarchies that operate in development. The relative simplicity and genetic tractability of organisms such as Dictyostelium will lead the way in the genomic analyses of multicellular development over the next five years.

描述：完整真核基因组序列的可用性 有望加速细胞和细胞基本机制的研究 发育生物学。在人们能够充分利用这一信息并进行研究之前 一个生物体的生物学必须从其整个基因组的背景来看 能够：（1）识别基因组内的相关信息，（2） 了解信息如何转化为功能，以及 (3) 整合 这些数据构成了一幅全面的生物图景。调查人员是 开发实验和理论工具，以促进 这些目标是研究所有生物体的目标。他们打算通过以下方式测试这些工具 分析土壤变形虫盘基网柄菌，并通过比较 与其他基因组分析中发现的结果相比较。该项目将 将技术开发和遗传资源获取结合起来 基于基因组的功能分析方法。最近发表的报告 提供充分的证据表明微阵列数据可以有效地用于 遗传分析（表型分析、上位性测试等）。调查人员的 基本思想是细胞的转录状态可以用作高分辨率 表型具有必要的区分能力来放置所有 双网柄菌中的基因位于框架调控网络内，从中， 可以计划适当的实验来测试分子机制。这 研究人员将检查生长过程中所有基因的表达 大约 5,000 个敲除突变体中的每一个都发育出双网柄菌。这 数据，当与来自平行表型和其他的适应度数据相结合时 传统的表型分析将使我们能够对基因进行可测试的预测 职能并提出监管网络建议。功能性的独特价值 基因组学依靠大型数据库的力量来提供广泛的、 大概是对生物系统的偏见较少。此方法将打开 以更广阔的视野探索发展。它将再次强调 整个有机体作为基本的生物单位并揭示 信号通路与细胞类型之间的关系无法确定 从小组基因的分析中得到赞赏。调查人员是 对盘基网柄菌生物学中常见的那些方面感兴趣 真核生物，这将为定义真核生物提供信息 个体基因的功能和调控层次的组织 在开发中运行。相对简单和遗传易处理性 盘基网柄菌等生物体将引领基因组分析 未来五年的多细胞发育。