The Dictyostelium Stock Center

盘基网柄菌库存中心

• 批准号: 8880401
• 负责人: REX L CHISHOLM
• 金额: $ 26.42万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2019-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8880401
• 关键词: Accounting; Address; Adhesions; Amoeba genus; Animal Model; Animals; Antibodies; Biocompatible Materials; Biological; Biological Preservation; Biomedical Research; Cell physiology; Characteristics; Charge; Chemotaxis; Cisplatin; Collection; Communication; Communities; Community Health Education; DNA; Development; Dictyostelium; Dictyostelium discoideum; Engineering; Ensure; Eukaryota; Evolution; Feedback; Fees; Funding; Gene Targeting; Genes; Genetic; Genome; Goals; Growth; Guidelines; Habits; Individual; International; Lead; Left; Life; Literature; Modeling; Modification; Mutation; Organism; Phagocytosis; Pharmaceutical Preparations; Phenotype; Plants; Plasmids; Policies; Positioning Attribute; Procedures; Protocols documentation; Published Comment; Publishing; Quality Control; Recovery; Relative (related person); Reporting; Research; Research Personnel; Research Project Grants; Resistance; Resource Sharing; Resources; Shipping; Ships; Side; Signal Transduction; Social Behavior; System; Testing; Time; Trees; United States National Institutes of Health; Update; Yeasts; base; cDNA Library; cell motility; comparative genomics; cost; cost effective; experience; functional genomics; human disease; improved; innovation; interest; meetings; microbial; mitochondrial dysfunction; model organisms databases; mutant; next generation; novel; pathogen; public health relevance; repository; response; social; usability; web interface

 DESCRIPTION (provided by applicant): The eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, signal transduction, cellular response to drugs, and host-pathogen interactions. Dictyostelium's genome includes genes with significant homology to vertebrate, yeast and microbial genes, attracting researchers interested in a wide variety of biological topics such as human disease, comparative genomics, and self/non-self-recognition. The Dictyostelium research community has significantly benefited from the Dicty Stock Center (DSC), a central repository of strains and plasmids that provides a cost-effective means of ensuring that strains are widely available and maintained in standardized conditions. The DSC enables new Dictyostelium researchers to obtain tested research materials and quickly start new research projects at minimal costs. The knowledgeable DSC staff provides technical support and protocols for effective handling of the strains. The DSC also benefits greatly from its synergistic integration with dictyBase, the model organism database (MOD) for Dictyostelium, which provides the web interface for strain and plasmid annotations, and allows strain orders to be submitted online. The DSC collects genetically modified strains of Dictyostelium and other species of social amoebae that contain novel phenotypes and which would be difficult to re-engineer. It also offers both expression and gene-targeting plasmids from experienced researchers that would be time consuming to reconstruct. This application seeks support to continue operating this community resource. The goals of this proposal are twofold: (1) Maintain, expand, and distribute the DSC collection by continuing to collect, annotate and distribute strains from Dictyostelium and related organisms, focusing on mutants that produce phenotypes and marked strains (such as GFP fusions) as well as useful plasmids, cDNA libraries, and antibodies following continuously updated standard operating procedures; (2) Create MyDSC to improve usability and utility for users by creating personalized user accounts, allowing users to change, update or delete their orders, request unavailable strains, and to leave comments pertaining to growth habits or phenotypes for each strain; this system will also facilitate communications between users and DSC staff, easing the workflow on both sides. Implementing these improvements will increase efficiency for DSC staff and greatly improve the online experience for users.

 描述（由申请人提供）：真核生物盘基网柄菌是一种广泛用于研究细胞过程的模型生物，例如细胞运动、趋化性、信号转导、细胞对药物的反应以及宿主与病原体相互作用的基因组。与脊椎动物、酵母和微生物基因的同源性，吸引了对人类疾病、比较基因组学等多种生物学主题感兴趣的研究人员盘基网柄菌研究界从 Dicty Stock Center (DSC) 中受益匪浅，该中心是菌株和质粒的中央存储库，提供了一种经济高效的方法，可确保菌株广泛可用并在标准化条件下保存。 DSC 使新的盘基网柄菌研究人员能够以最低的成本获得经过测试的研究材料并快速启动新的研究项目。知识丰富的 DSC 工作人员为有效处理菌株提供技术支持和方案。与盘基网柄菌的模式生物数据库 (MOD) dictyBase 协同集成，提供菌株和质粒注释的网络界面，并允许在线提交菌株订单。 DSC 收集盘基网柄菌和其他社会变形虫物种的转基因菌株。它还提供了来自经验丰富的研究人员的表达和基因靶向质粒，而这些质粒的重建非常耗时。该申请寻求支持以继续运营该社区资源：(1) 通过继续收集、注释和分发盘基网柄菌属和相关生物体的菌株来维护、扩展和分发 DSC 集合，重点关注以下突变体： (2) 创建 MyDSC 通过创建个性化用户帐户来提高用户的可用性和实用性，允许用户可以更改、更新或删除订单，请求不可用的菌株，并留下有关每种菌株的生长习性或表型的评论；该系统还将促进用户和 DSC 工作人员之间的沟通，从而简化双方的工作流程。将提高DSC工作人员的工作效率，并极大改善用户的在线体验。