Core C

核心C

• 批准号: 7407113
• 负责人: John D Groopman
• 金额: $ 10.58万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7407113
• 关键词: Address; Area; Arts; Bioconductor; Bioinformatics; Biostatistics Core; Core Facility; Data Analyses; Environmental Exposure; Epigenetic Process; Future; Gene Chips; Gene Expression; Genes; Goals; Grant; Human Genome; Instruction; Methods; Molecular Biology; Molecular Genetics; Proteomics; Rattus; Research Infrastructure; Research Personnel; Resources; Technology; Training; functional genomics; genetic analysis; instrumentation; medical schools; member; mouse genome; tool

B3. Molecular Genetics and Epigenetics The Molecular Genetics and Epigenetics component of the IHSFC, formerly called Facility Core C: Microarray and Molecular Biology, has been transformed to address the expressed current and future needs of Center members for instrumentation and expertise support in the areas of gene expression, epigenetics and functional genomics. The goal of this activity is to provide to Center members access to cutting edge technologies in the analysis of gene expression and modulation through access to instrumentation, instruction, assistance in methods and training in the analysis of data. We have added the epigenetics analysis to this iteration of the grant, replacing resources that had been previously directed towards proteomics. This decision was made because the Proteomics Core at Johns Hopkins School of Medicine is (http://www.hopkinsmedicine.org/msf/) now widely available for Center members and we choose not to duplicate these efforts. The objectives of this component of the IHSFC are to: ¿ provide state-of-the-art analysis of gene expression and analysis using the Affymetrix platform for mouse (genome 430A 2.0 array), rat (expression 230 2.0) and human (genome U133 Plus 2.0 array) gene chips that comprise the global gene profiling for these respective species; ¿ provide infrastructure so that an investigator can understand the global gene expression changes modified by environmental exposures that are manifested through epigenetic changes; ¿ integrate with the Bioinformatics/Biostatistics core for using Bioconductor and other tools for the analysis of genetic and epigenetic changes.

B3. 分子遗传学和表观遗传学 IHSFC 的分子遗传学和表观遗传学部分，以前称为 Facility Core C： 微阵列和分子生物学已经转变，以满足当前和未来的明确需求 中心成员在基因表达、表观遗传学和 这项活动的目标是为中心成员提供最前沿的知识。 通过使用仪器、指令来分析基因表达和调节的技术， 我们在数据分析方法和培训方面提供了帮助。 拨款的迭代，取代了之前用于蛋白质组学的资源。 之所以成立是因为约翰霍普金斯大学医学院的蛋白质组学核心是 (http://www.hopkinsmedicine.org/msf/) 现已广泛供中心会员使用，但我们选择不提供 IHSFC 该部分的目标是： ¿使用 Affymetrix 平台为小鼠提供最先进的基因表达分析和分析 （基因组 430A 2.0 阵列）、大鼠（表达 230 2.0）和人类（基因组 U133 Plus 2.0 阵列）基因芯片 其中包括这些各自物种的全球基因图谱； ¿提供基础设施，以便研究人员能够了解修改后的全局基因表达变化 通过表观遗传变化表现出来的环境暴露； ¿与生物信息学/生物统计学核心集成，使用 Bioconductor 和其他工具进行分析 遗传和表观遗传变化。