Development of Aersolized Vaccines

雾化疫苗的开发

• 批准号: 7592437
• 负责人: Mario Roederer
• 金额: $ 45.97万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592437
• 关键词: Address; Adenoviruses; Antibodies; Antibody Formation; Antigens; Area; CD8B1 gene; Depth; Development; Generations; Goals; HIV; Immune response; Immunization; Influenza; Lung; Macaca mulatta; Mucosal Immune Responses; Process; Recombinants; Rest; Route; Site; Surface; T-Lymphocyte; Testing; Tuberculosis; Vaccines; aerosolized; immunogenic; mucosal site; novel strategies; pathogen; response; vector

We have demonstrated that aerosolized recombinant adenovirus is highly immunogenic when delivered to the lung of rhesus macaques. Specifically, this immunization generates very high levels of persistent CD4 and CD8 T cell responses in the lung, although very low levels throughout the rest of the body. Interestingly, we also find very good generation of antibody responses, both throughout the body and at various mucosal sites. Thus, this route may be very important for the generation of mucosal immune responses needed to protect against challenges such as HIV. But certainly, the high lung cellular responses are ideal for the possible protection against tuberculosis or influenza; we are in the process of immunizing with vectors against these pathogens and testing whether or not protection is seen.

我们已经证明，将雾化的重组腺病毒具有高度免疫原性，当时将其输送到恒河猕猴的肺部。 具体而言，这种免疫在肺部产生非常高的持续CD4和CD8 T细胞反应，尽管整个身体的其余部分都非常低。 有趣的是，我们还发现整个身体和各种粘膜部位的抗体反应都非常好。 因此，这种途径对于保护诸如艾滋病毒之类的挑战所需的粘膜免疫反应可能非常重要。 但是，当然，高肺细胞反应是防止结核病或流感的理想选择。我们正在用载体对这些病原体进行免疫接种，并测试是否看到保护。