Non-Human Primate Core

非人类灵长类核心

基本信息

批准号：
8946615
负责人：
Mario Roederer
金额：
$ 68.97万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8946615
关键词：
Adjuvant Animal Care and Use Committees Animals Basic Science Biological Assay Cell Separation Clinical Collaborations Consult Contracts Cryopreservation Data Data Analyses Ensure Evaluation Flow Cytometry Generations Human IACUC Institutes International Laboratories Laboratory Animal Medicine Lymphocyte Maintenance Memory Methods Modeling Oral Pathogenesis Practice Guidelines Preclinical Testing Procedures Protocols documentation Qualifying Reproducibility Research Personnel Research Support SIV Schedule Site Specimen Specimen Handling Technology Testing Tissues Training Translational Research Translations Vaccination Vaccine Research Vaccines Virus Diseases Writing design good laboratory practice immune function immunogenicity mucosal site nonhuman primate operation preclinical study research study response sample collection trafficking vector

项目摘要

The Vaccine Research Center Nonhuman primate Immunogenicity Core supports the basic research laboratories of the VRC that are performing vaccination experiments with nonhuman primates. The Core has developed rigorous protocols for the isolation of cells, cryopreservation of cells, isolation of mucosal specimens, assays to detect immune function, and assays to determine lymphocyte representation in tissues. The NIC will qualify and validate these assays in order to provide the most rigorous support for basic research laboratories by ensuring consistency, sensitivity, and reproducibility. The Core has expertise in all aspects of NHP experiments, and provides support to investigators at multiple levels: design and implementation of experiments, writing and presenting the animal protocols to the Institutional Animal Care and Use Committee, scheduling of experiments, interfacing with the Laboratory of Animal Medicine and animal contract facilities, processing of specimens, data analysis. The Core also maintains a cryorepository of specimens, and participates in international training and collaborations to ensure translation of Core assays to other sites.

疫苗研究中心非人灵长类动物免疫原性核心为 VRC 的基础研究实验室提供支持，这些实验室正在进行非人灵长类动物疫苗接种实验。该核心开发了严格的细胞分离、细胞冷冻保存、粘膜样本分离、检测免疫功能的测定以及确定组织中淋巴细胞代表性的测定的严格方案。 NIC 将鉴定和验证这些检测方法，以确保一致性、灵敏度和可重复性，为基础研究实验室提供最严格的支持。核心拥有 NHP 实验各个方面的专业知识，并为研究人员提供多个级别的支持：实验的设计和实施、向机构动物护理和使用委员会编写和提交动物方案、实验安排、与实验室的接口。动物医学和动物合同设施、标本处理、数据分析。该核心还维护着一个标本冷冻库，并参与国际培训和合作，以确保将核心检测转移到其他地点。