Non-Human Primate Core

非人类灵长类核心

基本信息

批准号：
9161791
负责人：
Mario Roederer
金额：
$ 68.97万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9161791
关键词：
Adjuvant Animal Care and Use Committees Animals Basic Science Biological Assay Cell Separation Clinical Collaborations Consult Contracts Cryopreservation Data Data Analyses Ensure Evaluation Flow Cytometry Generations Human IACUC Institutes International Laboratories Laboratory Animal Medicine Lymphocyte Maintenance Memory Methods Modeling Oral Pathogenesis Practice Guidelines Preclinical Testing Procedures Protocols documentation Qualifying Reproducibility Research Personnel Research Support SIV Schedule Site Specimen Specimen Handling Technology Testing Tissues Training Translational Research Translations Vaccination Vaccine Research Vaccines Virus Diseases Writing design good laboratory practice immune function immunogenicity mucosal site nonhuman primate operation preclinical study research study response sample collection trafficking vector

项目摘要

The Vaccine Research Center Nonhuman primate Immunogenicity Core supports the basic research laboratories of the VRC that are performing vaccination experiments with nonhuman primates. The Core has developed rigorous protocols for the isolation of cells, cryopreservation of cells, isolation of mucosal specimens, assays to detect immune function, and assays to determine lymphocyte representation in tissues. The NIC will qualify and validate these assays in order to provide the most rigorous support for basic research laboratories by ensuring consistency, sensitivity, and reproducibility. The Core has expertise in all aspects of NHP experiments, and provides support to investigators at multiple levels: design and implementation of experiments, writing and presenting the animal protocols to the Institutional Animal Care and Use Committee, scheduling of experiments, interfacing with the Laboratory of Animal Medicine and animal contract facilities, processing of specimens, data analysis. The Core also maintains a cryorepository of specimens, and participates in international training and collaborations to ensure translation of Core assays to other sites.

疫苗研究中心非人类灵长类动物免疫性核心支持正在使用非人类灵长类动物进行疫苗接种实验的VRC的基础研究实验室。该核心已开发出严格的方案，用于分离细胞，细胞冷冻保存，粘膜样本的分离，检测免疫功能的测定以及确定组织中淋巴细胞表示的测定法。 NIC将通过确保一致性，敏感性和可重复性来为基础研究实验室提供最严格的支持和验证这些测定法。该核心在NHP实验的各个方面都有专业知识，并在多个层面上为研究人员提供了支持：实验的设计和实施，为机构动物护理和使用委员会撰写和介绍动物方案，安排实验，与动物医学和动物医学实验室进行交互，与动物合同设施交往，样品的处理，数据分析。该核心还维护了标本的冷冻量，并参加了国际培训和合作，以确保将核心测定法转换为其他站点。