Mosquito innate immune responses to arbovirus infections

蚊子对虫媒病毒感染的先天免疫反应

• 批准号: 7675036
• 负责人: Anthony A. James
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675036
• 关键词: Aedes; Arbovirus Infections; Arboviruses; Bacterial Infections; Code; Culicidae; Dengue; Development; Emerging Communicable Diseases; Epidemiology; Gene Amplification; Gene Expression; Genes; Genetic; Genetic Transcription; Genome; Goals; Health; Human; Immune response; Infection; Infectious Diseases Research; Knowledge; Methods; Molecular Profiling; Pattern; Proteins; Regulation; Reporter; Research; Role; Testing; Transgenes; Untranslated RNA; Viral; Viral Vector; Virus; Virus Diseases; Yellow Fever; base; biodefense; chikungunya; design; disorder control; in vivo; member; novel; pathogen; promoter; response; transmission process; vector; vector mosquito

The genes involved in mosquito immune responses to parasitic and bacterial infections have been studied extensively, however similar knowledge of vector responses to viruses is limited. Our long-term goal is to use knowledge of mosquito responses to viruses to design novel disease-control methods. This proposed research focuses on the development and usage of whole-genome tiling arrays for analyses of gene expression in the mosquito vector, Aedes aegypti, following infection with arboviruses. The information generated by this study will reveal global and specific responses of mosquitoes to a number of viral pathogens, and provide materials for the development of genetics-based disease control strategies. The Specific Aims are: i) to design whole-genome tiling arrays containing interrogation probes for the genomes of Ae. aegypti, and the arboviruses, Dengue, Yellow Fever and Chikungunya; 2) to identify mosquito protein-coding and noncoding RNAs with altered transcription patterns following infection with viruses and validate their expression profiles by quantitative gene amplification; and 3) to design transgenes expressing reporter molecules under the regulation of virus infection-induced mosquito promoters, and test in vivo the functionality of the control sequences.

与蚊子对寄生虫和细菌感染的免疫反应有关的基因已被 进行了广泛的研究，但是关于载体对病毒的反应的类似知识仍然有限。我们的长期目标 是利用蚊子对病毒反应的知识来设计新的疾病控制方法。这个提议 研究重点是用于基因表达分析的全基因组平铺阵列的开发和使用 在蚊媒埃及伊蚊感染虫媒病毒后。生成的信息 这项研究将揭示蚊子对多种病毒病原体的整体和特定反应，并提供 用于制定基于遗传学的疾病控制策略的材料。具体目标是： i) 设计包含伊蚊基因组询问探针的全基因组平铺阵列。埃及伊蚊，以及 虫媒病毒、登革热、黄热病和基孔肯雅热； 2）识别蚊子蛋白质编码和非编码 病毒感染后转录模式发生改变的 RNA 并验证其表达 通过定量基因扩增进行分析； 3) 设计表达报告分子的转基因 病毒感染诱导的蚊子启动子的调节，并在体内测试对照的功能 序列。