RECOMBINANT ANTIBODIES BLOCKING SPOROZOITE TRANSMISSION

阻断子孢子传播的重组抗体

• 批准号: 6497133
• 负责人: Anthony A. James
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497133
• 关键词: Aedes; Coccidia; Plasmodium; arthropod borne communicable disease; blocking antibody; cell line; chickens; communicable disease control; communicable disease transmission; disease vectors; gene expression; genetic manipulation; host organism interaction; immunoglobulin structure; malaria; molecular cloning; recombinant proteins; spores; transfection; western blottings

DESCRIPTION (Adapted from the Applicant's Abstract): This is a 3 year revised application that proposes to provide a working model using antibodies, Fab's/F(ab')2's or scFvs to block sporozoite (from P. gallinaceum) invasion of salivary glands in mosquitoes (A. aegypti). The ultimate goal is to be able to insert genes for these scFv's into mosquitoes using recombinant methods so that the mosquitoes will not be suitable hosts for sporozoite invasion and transmission. The idea is that results from these research projects will be relatively easily transferred to the P. falciparum sporozoites and their vectors, anopholine mosquitoes.

描述（改编自申请人的摘要）：这是一个为期 3 年的修订版 提议提供使用抗体的工作模型的应用程序， Fab's/F(ab')2's 或 scFvs 可阻止子孢子（来自鸡 P. gallinaceum）入侵 蚊子（埃及伊蚊）的唾液腺。最终目标是能够 使用重组方法将这些 scFv 的基因插入蚊子中，以便 蚊子不是子孢子入侵的合适宿主 传播。这个想法是这些研究项目的结果将 相对容易转移到恶性疟原虫子孢子及其 媒介，按蚊。