Species-specific regulated transposition in mosquitoes

蚊子中物种特异性调节转座

• 批准号: 6992692
• 负责人: Anthony A. James
• 金额: $ 36.91万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6992692
• 关键词: Aedes; Anopheles; arthropod borne communicable disease; arthropod genetics; arthropod nonpollutant control; communicable disease control; communicable disease transmission; disease /disorder prevention /control; disease vectors; genetic strain; genetically modified animals; insecticide resistance; laboratory mouse; reproduction; synthetic nucleotide; transcription factor; transfection /expression vector; transposon /insertion element

DESCRIPTION (provided by applicant): Vector-borne diseases cause over one million deaths annually in people worldwide. The long term objective of this research is to develop strains of vector mosquitoes that are genetically refractory to the transmission of pathogens such as those that cause malaria and dengue fever. These insects will be used to test the hypothesis that an increase in the frequency of a gene or allele that confers decreased vector competence to a population of mosquitoes will lead to a reduction in the incidence and prevalence of that disease. Following the successful efforts of our laboratory and others demonstrating the possibilities for engineering synthetic effector genes that produce pathogen refractory phenotypes in mosquitoes, we now propose to investigate specific drive mechanisms that would facilitate the safe and efficient spread of such genes in vector mosquito populations. The SPECIFIC AIMS of this application have been designed to take advantage of the progress made with mosquito transgenesis and the fundamental nature of embryonic development in insects, to meet the need to research gene drive mechanisms. The SPECIFIC AIMS are to: 1) Identify the cis-acting DNA and promoter elements responsible for the specific expression patterns of the nanos (nos) orthologous genes in Anopheles gambiae, An. stephensi and Aedes aegypti; 2) Construct and test synthetic transposable elements composed of mosquito nos genes and the Mos1 and piggyBac transposons; and 3) Determine if synthetic transposons can spread in caged populations of An. gambiae and Ae. aegypti.

描述（由申请人提供）：媒介传播疾病每年导致全世界超过一百万人死亡。这项研究的长期目标是开发出对传播病原体（例如引起疟疾和登革热的病原体）具有遗传抵抗力的媒介蚊子品种。这些昆虫将被用来检验这样一个假设：基因或等位基因频率的增加会降低蚊子种群的媒介能力，从而降低该疾病的发病率和流行率。我们的实验室和其他实验室的成功努力证明了工程合成效应基因在蚊子中产生病原体难治性表型的可能性，我们现在建议研究特定的驱动机制，以促进此类基因在媒介蚊子种群中安全有效地传播。本申请的具体目标旨在利用蚊子转基因取得的进展和昆虫胚胎发育的基本性质，以满足研究基因驱动机制的需要。具体目标是： 1) 鉴定冈比亚按蚊中负责 nanos (nos) 直系同源基因特定表达模式的顺式作用 DNA 和启动子元件。斯蒂芬斯和埃及伊蚊； 2）构建并测试由蚊子nos基因和Mos1和piggyBac转座子组成的合成转座元件； 3) 确定合成转座子是否可以在笼养的按蚊群体中传播。冈比亚和伊蚊。埃及伊蚊。