The Aedes aegypti Genome Project

埃及伊蚊基因组计划

• 批准号: 6579559
• 负责人: DAVID W SEVERSON
• 金额: $ 108.1万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6579559
• 关键词: Aedes; Anopheles; animal genetic material tag; arthropod borne communicable disease; artificial chromosomes; biotechnology; chickens; dengue virus; disease vectors; fluorescent in situ hybridization; genetic library; genetic mapping; genome; gerbil /jird; guinea pigs; high throughput technology; laboratory mouse; laboratory rat; malaria; molecular cloning; nucleic acid sequence; polymerase chain reaction; sequence tagged sites

DESCRIPTION (provided by applicant): Arthropod-borne viral diseases have re-emerged as major health problems in most tropical countries. For example, the incidence of dengue fever has increased greatly over the past 20 years, recently including its hemorrhagic form with associated human fatalities. The mosquito Aedes aegypti is the primary vector. In addition, A. aegypti is an excellent laboratory model for examining vector interaction with a malaria parasite (Plasmodium gallinaceum) and a lymphatic filarioid nematode (Brugia malayi). Whole genome sequence data is available for humans, Drosophila melanogaster, the malaria parasite vector Anopheles gambiae, the malaria parasite Plasmodium falciparum, and several other parasite genome projects are underway. This proposal seeks to initiate an A. aegypti genome project, with an expectation that complete knowledge of the mosquito vector, parasite and human genomes will promote and foster new disease control strategies. The immediate objectives of this project are to: (1) sequence both ends of 40,000 cDNAs derived from normalized libraries produced from 4 specific mRNA sources (from pooled mRNAs isolated from various developmental stages and adult tissues and from females exposed to infected blood meals containing dengue virus, a malaria parasite, and a filarial worm parasite), (2) to sequence both ends of 50,000 genomic DNA Bacterial Artificial Chromosome (BAC) clones, and (3) to use fluorescent in situ hybridization (FISH) technology to physically map 1000 A. aegypti BAC clones to metaphase chromosomes. These aims will develop a body of genome information including EST sequences, genomic sequences, and the physical map positions of large genomic clones that will enhance gene discovery, and also provide critical tools for an eventual complete genome sequencing project. The proposal complements a nearly completed genome project to examine the primary malaria vector, Anopheles gambiae, as the 2 species reflect the range of genome size and organization among mosquito species. The long range goal is to promote and foster collaboration between funding agencies to support an effort to sequence the entire A. aegypti genome.

描述（由申请人提供）： 节肢动物传播的病毒性疾病已重新成为大多数热带国家的主要健康问题。例如，过去 20 年来，登革热的发病率大幅增加，最近还包括导致人类死亡的出血性登革热。埃及伊蚊是主要媒介。此外，埃及伊蚊是检查媒介与疟原虫（鸡疟原虫）和淋巴丝状线虫（马来丝虫）相互作用的优秀实验室模型。人类、黑腹果蝇、疟疾寄生虫载体冈比亚按蚊、疟原虫恶性疟原虫的全基因组序列数据已经可用，其他几种寄生虫基因组项目正在进行中。该提案旨在启动埃及伊蚊基因组项目，期望对蚊子媒介、寄生虫和人类基因组的完整了解将促进和培育新的疾病控制策略。该项目的直接目标是：(1) 对 40,000 个 cDNA 的两端进行测序，这些 cDNA 源自 4 个特定 mRNA 来源（从不同发育阶段和成体组织中分离出的汇集 mRNA，以及暴露于含有登革热的感染血粉的女性）产生的标准化文库。病毒、疟疾寄生虫和丝虫寄生虫），(2) 对 50,000 个基因组 DNA 细菌人工染色体 (BAC) 的两端进行测序(3) 使用荧光原位杂交 (FISH) 技术将 1000 个埃及伊蚊 BAC 克隆物理定位到中期染色体。这些目标将开发一系列基因组信息，包括 EST 序列、基因组序列和大型基因组克隆的物理图谱位置，这将增强基因发现，并为最终的完整基因组测序项目提供关键工具。该提案补充了即将完成的基因组计划，该计划旨在检查主要疟疾媒介冈比亚按蚊，因为这两个物种反映了蚊子物种之间的基因组大小和组织范围。长期目标是促进和促进资助机构之间的合作，以支持对整个埃及伊蚊基因组进行测序的工作。