Structure and functions of the actin cytoskeleton

肌动蛋白细胞骨架的结构和功能

• 批准号: 10794592
• 负责人: Tatyana Svitkina
• 金额: $ 25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-16 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794592
• 关键词: Actins; Administrative Supplement; Cytoskeleton; Equipment; Fluorescence; Funding; Goals; Image; Lighting; Microscope; Microscopy; Productivity; Research; Structure; Technology; fighting; human disease; novel diagnostics; parent grant; tool; ultra high resolution

Summary In this Administrative Supplement for Equipment application I request funds to purchase a superresolution fluorescence module (DeepSIM) installed on Nikon inverted microscope to perform subdiffraction imaging using SIM (Structured Illumination Microscopy) technology in order to perform research at the high technological level demanded by the current state of the field. This equipment is needed to achieve goals of the parent grant R35 GM 140832 entitled “Structure and functions of the actin cytoskeleton”.

概括 在本设备申请行政补充中，我请求资金购买超分辨率 安装在尼康倒置显微镜上的荧光模块（DeepSIM）进行亚衍射成像 使用SIM（结构照明显微镜）技术来进行高水平的研究 该设备需要达到该领域当前状态所需的技术水平才能实现以下目标。 母基金R35 GM 140832，题为“肌动蛋白细胞骨架的结构和功能”。

项目成果

Feedback between mechanosensitive signaling and active forces governs endothelial junction integrity.

• DOI: 10.1038/s41467-022-34701-y
• 发表时间: 2022-11-19
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: McEvoy, Eoin;Sneh, Tal;Moeendarbary, Emad;Javanmardi, Yousef;Efimova, Nadia;Yang, Changsong;Marino-Bravante, Gloria E.;Chen, Xingyu;Escribano, Jorge;Spill, Fabian;Manuel Garcia-Aznar, Jose;Weeraratna, Ashani T.;Svitkina, Tatyana M.;Kamm, Roger D.;Shenoy, Vivek B.
• 通讯作者: Shenoy, Vivek B.

Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges.

• DOI: 10.1038/s41467-022-33852-2
• 发表时间: 2022-10-17
• 期刊: Nature communications
• 影响因子: 16.6

Adenomatous Polyposis Coli (APC) in cell migration.

• DOI: 10.1016/j.ejcb.2022.151228
• 发表时间: 2022-06
• 期刊: EUROPEAN JOURNAL OF CELL BIOLOGY
• 影响因子: 6.6
• 作者: Fang, Xingyuan;Svitkina, Tatyana M.
• 通讯作者: Svitkina, Tatyana M.

A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion.

• DOI: 10.1038/s41467-023-42661-0
• 发表时间: 2023-10-28
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Liu, Di-Ao;Tao, Kai;Wu, Bin;Yu, Ziyan;Szczepaniak, Malwina;Rames, Matthew;Yang, Changsong;Svitkina, Tatyana;Zhu, Yueyao;Xu, Fengyuan;Nan, Xiaolin;Guo, Wei
• 通讯作者: Guo, Wei

Imaging Cytoskeleton Components by Electron Microscopy.

• DOI: 10.1007/978-1-0716-1661-1_2
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)