SyncroPatch 384 Automated Patch Clamp Instrument

SyncroPatch 384 自动膜片钳仪器

• 批准号: 10721590
• 负责人: Jen Qian Pan
• 金额: $ 60万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-17 至 2024-07-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721590
• 关键词: 7 year old; Amplifiers; Area; Biological Assay; Boston; Closure by clamp; Data Analyses; Electrophysiology (science); Funding; Genetic Diseases; Head; Human; Individual; Institution; Ion Channel; Liquid substance; Manuals; Measurement; National Institute of Mental Health; Neurons; Operating System; Patch-Clamp Techniques; Pharmacology Study; Property; Research Personnel; Research Project Grants; Resistance; Robotics; Running; Scientist; Technology; Temperature; Variant; cell type; drug discovery; instrument; manufacture; patch clamp; seal; success; user friendly software; voltage clamp

The objective of this proposal is to enhance the capability of a diverse group of researchers funded by National Institute of Mental Health in the greater Boston area to perform electrophysiological assays for the purposes of drug discovery, targeted pharmacological studies, determining the functional consequences of human ion channel variants associated with genetic diseases, and investigating the electrophysiological properties of human neurons. This objective will be achieved by the purchase and installation of a 384-well automated planar patch clamp instrument (SyncroPatch 384) manufactured by Nanion Technologies. This instrument will replace the 7-year-old legacy instrument (SyncroPatch 384 PE) this is approaching obsolescence and lacks critically important features that are needed by researchers. The Nanion SyncroPatch 384 instrument consists of a patch clamp module (measurement chamber) integrated with a state-of-the-art robotic liquid handler with a 384-pipetting head (Biomek i5), 384 individual patch clamp amplifiers that can operate in either voltage-clamp or current-clamp mode, advanced temperature control with range 10°C to 37°C, and powerful user-friendly software for instrument control and data analysis that runs under a supported operating system. The instrument allows for formation of giga-Ohm resistance seals with a variety of cell types and produces electrophysiological recordings with high fidelity that are comparable to the traditional manual patch clamp technique but at two orders of magnitude greater throughput. The instrument will be managed by the ICE-T (ion channel and electrogenic transporter) platform at Broad Institute. Strong institutional support for the instrument and its deployment will be provided, and effective oversight will be achieved by a team of professional scientist. The instrument is expected to enhance the success of several NIMH - funded research projects and provide a new platform for active and future research.

该提案的目的是增强由资助的潜水者的能力 大波士顿地区的国家心理健康研究所，以进行电生理评估 药物发现的目的，有针对性的药物研究，确定功能 与遗传疾病相关的人离子通道变异的后果，并研究了 人神经元的电生理特性。此目标将通过购买和 由384孔自动平面贴片夹仪器（Syncropatch 384）安装 纳米技术。该乐器将取代7年历史的旧仪器（Syncropatch 384 pe）​​这正接近过时，并且缺乏至关重要的特征 研究人员。 Nanion Syncropatch 384仪器由一个贴片夹模块（测量）组成 室）与最先进的机器人液体处理程序集成，带有384个流头（Biomek i5）， 384个可以在电压夹或电流钳模式下运行的单个贴片夹放大器， 范围为10°C至37°C的高级温度控制，以及强大的用户友好软件 在受支持的操作系统下运行的仪器控制和数据分析。乐器 允许形成具有多种细胞类型的Giga-Ohm电阻密封件，并产生 具有高保真度的电生理记录，可与传统手动补丁相媲美 夹紧技术，但在两个数量级的吞吐量上。该仪器将由 Broad Institute的ICE-T（离子通道和电转运蛋白）平台。强大的制度 将提供对该工具及其部署的支持，并将实现有效的监督 专业科学家团队。该仪器有望增强几个NIMH的成功 - 资助研究项目，并为积极和未来的研究提供了一个新的平台。