SyncroPatch 384 Automated Patch Clamp Instrument

SyncroPatch 384 自动膜片钳仪器

• 批准号: 10721590
• 负责人: Jen Qian Pan
• 金额: $ 60万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-17 至 2024-07-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721590
• 关键词: 7 year old; Amplifiers; Area; Biological Assay; Boston; Closure by clamp; Data Analyses; Electrophysiology (science); Funding; Genetic Diseases; Head; Human; Individual; Institution; Ion Channel; Liquid substance; Manuals; Measurement; National Institute of Mental Health; Neurons; Operating System; Patch-Clamp Techniques; Pharmacology Study; Property; Research Personnel; Research Project Grants; Resistance; Robotics; Running; Scientist; Technology; Temperature; Variant; cell type; drug discovery; instrument; manufacture; patch clamp; seal; success; user friendly software; voltage clamp

The objective of this proposal is to enhance the capability of a diverse group of researchers funded by National Institute of Mental Health in the greater Boston area to perform electrophysiological assays for the purposes of drug discovery, targeted pharmacological studies, determining the functional consequences of human ion channel variants associated with genetic diseases, and investigating the electrophysiological properties of human neurons. This objective will be achieved by the purchase and installation of a 384-well automated planar patch clamp instrument (SyncroPatch 384) manufactured by Nanion Technologies. This instrument will replace the 7-year-old legacy instrument (SyncroPatch 384 PE) this is approaching obsolescence and lacks critically important features that are needed by researchers. The Nanion SyncroPatch 384 instrument consists of a patch clamp module (measurement chamber) integrated with a state-of-the-art robotic liquid handler with a 384-pipetting head (Biomek i5), 384 individual patch clamp amplifiers that can operate in either voltage-clamp or current-clamp mode, advanced temperature control with range 10°C to 37°C, and powerful user-friendly software for instrument control and data analysis that runs under a supported operating system. The instrument allows for formation of giga-Ohm resistance seals with a variety of cell types and produces electrophysiological recordings with high fidelity that are comparable to the traditional manual patch clamp technique but at two orders of magnitude greater throughput. The instrument will be managed by the ICE-T (ion channel and electrogenic transporter) platform at Broad Institute. Strong institutional support for the instrument and its deployment will be provided, and effective oversight will be achieved by a team of professional scientist. The instrument is expected to enhance the success of several NIMH - funded research projects and provide a new platform for active and future research.

该提案的目的是提高由资助的多元化研究人员群体的能力 大波士顿地区的国家心理健康研究所将进行电生理学检测 药物发现的目的、靶向药理学研究、确定功能 人类离子通道变异与遗传疾病相关的后果，并调查 人类神经元的电生理特性将通过购买和实现。 安装 384 孔自动化平面膜片钳仪器 (SyncroPatch 384) Nanion Technologies。该仪器将取代已有 7 年历史的传统仪器 (SyncroPatch 384)。 PE）这已接近过时，并且缺乏所需的极其重要的功能 Nanion SyncroPatch 384 仪器由膜片钳模块（测量）组成。 室）与带有 384 移液头（Biomek i5）的最先进的机器人液体处理器集成， 384 个单独的膜片钳放大器，可以在电压钳或电流钳模式下运行， 先进的温度控制范围为 10°C 至 37°C，以及强大的用户友好型软件 在支持的操作系统下运行的仪器控制和数据分析。 允许与各种细胞类型形成千兆欧姆电阻密封并产生 电生理记录的高保真度可与传统手动贴片相媲美 钳技术，但仪器的吞吐量将提高两个数量级。 Broad Institute 的 ICE-T（离子通道和电转运蛋白）平台。 将对该文书及其部署提供支持，并通过以下方式实现有效监督： 该仪器预计将提高多个 NIMH 的成功率。 资助研究项目，并为积极和未来的研究提供新的平台。