Reconfigurable 3D Origami Probes for Multi-modal Neural Interface

用于多模态神经接口的可重构 3D 折纸探针

• 批准号: 10738994
• 负责人: GYORGY BUZSAKI
• 金额: $ 383.76万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10738994
• 关键词: 3-Dimensional; Address; Adhesives; Affect; Alloys; Amplifiers; Animals; Applications Grants; Area; Biochemical; Brain; Brain region; Chronic; Custom; Data; Detection; Development; Devices; Dopamine; Electrodes; Electrophysiology (science); Ensure; Fiber; Fiber Optics; Goals; Hormones; Letters; Magnetism; Measures; Metabolism; Methods; Mission; Modality; Monitor; Nature; Neuromodulator; Neurons; Neurosciences; Neurotransmitters; Noise; Optics; Pattern; Photometry; Physiological; Polymers; Positioning Attribute; Process; Records; Regulation; Reporting; Resolution; Rodent; Sampling; Scheme; Signal Transduction; Silicon; Site; Specificity; Surface Tension; Techniques; Technology; Temperature; Testing; Thinness; Time; Validation; Variant; Work; aqueous; awake; cell type; density; design; electric impedance; experimental study; fabrication; flexibility; in vivo; innovation; integrated circuit; magnetic field; multimodality; neural; neural circuit; neurotechnology; neurotransmission; novel; operation; optical fiber; programs; prototype; sensor; technology platform; tool; tool development

Project Summary Over the decades, various neurotechnologies have made significant advancements to meet the highest priority goals enumerated in the BRAIN 2025 Report. However, each tool development has been driven by a certain type of specific modalities in the target brain region, cell type, and limited experiments and has focused on scaling in a narrow domain. While the utility of such tools has uniquely served one modality, neuroscience experiments are inherently limited by the breadth of observations available. It is known that brain activities and status are affected by biophysiological parameters such as metabolism, hormones, neurotransmitters, temperature regulation, etc. However, as of yet, neurotechnology tools have not been combined to simultaneously measure multiple signals in a single experiment. This grant application proposes a reconfigurable neural interface platform on which multi-modal probes can be seamlessly integrated on the same flexible substrate to substantially enhance observational breadth. The proposed tool offers multi-modal capabilities by adding physio-bio-chemical sensor modules to the basic key electrophysiological functions of high-density neural recording and target-specific modulation of neurons. Each sensor module can be easily integrated into the existing modalities as an “additive” option, not a “replacing” alternative. The proposed platform is highly reconfigurable to meet the needs by pick-and-choose the desired modality for target studies and experiments. By modularizing and combing single-modality flexible probes, the proposed scheme can provide a highly efficient solution to the challenges in the comprehensive integration of multi-modality on the same platform. Preliminary Data: The previous work has demonstrated the feasibility of neuron-sized μLEDs (15 μm x 10 μm) monolithically integrated on silicon recording probes, precisely positioned relative to the recording sites. Prototype probes incorporated 256 recording sites and 128 μLEDs. Recently, we integrated the μLEDs on a flexible polymer substrate and stacked them with a recording probe. Initial feasibility was demonstrated for sensing dopamine and local brain temperature in-vivo from the flexible probes. Specific Aims: In aim 1, self-aligned flexible 3D origami probes will be developed for diverse neural interfaces with multiple modalities. Two prototype platforms will be developed: One is planar, stacked multi-modal probes with all electrical interfaces, and the other is 3D origami probes wrapped around an optical fiber that will give additional opto-modality such as photometry. In aim 2, a modular, compact headstage will be developed utilizing an innovative adaptable cable-on-chip assembly with custom ICs as an interposer. In aim 3, validation of the proposed multi-modal origami probes will be conducted in chronic rodent experiments.

项目概要 几十年来，各种神经技术取得了重大进展，以实现 BRAIN 2025 报告中列举的最高优先目标。然而，每种工具的开发都是由目标大脑区域、细胞类型和有限实验的某种特定模式驱动的。虽然这些工具的效用只适用于一种模式，但神经科学实验本质上受到可用观察范围的限制，众所周知，大脑活动和状态受到生物生理参数的影响。例如新陈代谢、激素、神经递质、温度调节等。然而，迄今为止，神经技术工具尚未结合起来在单个实验中同时测量多个信号。该资助申请提出了一个可重构的神经接口平台，在该平台上可以使用多模态探针。可以无缝集成在同一柔性基板上，以显着增强观察广度。该工具通过将生理生化传感器模块添加到高密度神经记录和特定目标的基本关键电生理功能中，提供多模式功能。每个传感器模块都可以作为“附加”选项轻松集成到现有模式中，而不是“替代”替代方案，该平台具有高度可重构性，可以通过选择所需的目标模式来满足需求。通过模块化和组合单模态柔性探针，所提出的方案可以为同一平台上多模态综合集成的挑战提供高效的解决方案。神经元大小的 μLED (15 μm x 10 μm) 单片集成在硅记录探针上的可行性，原型探针包含 256 个记录位点和 128 μLED。将它们与记录探针堆叠起来，证明了通过柔性探针在体内感测多巴胺和局部大脑温度的可行性：在目标 1 中，将开发用于多种模态的多种神经接口的自对准柔性 3D 折纸探针，将开发两个原型平台：一个是具有所有电气接口的平面堆叠多模态探针，另一个是包裹在光学器件上的 3D 折纸探针。在目标 2 中，将利用创新的适应性片上电缆组件和定制 IC 作为光度测量技术，开发模块化、紧凑的探头。在目标 3 中，将在慢性啮齿动物实验中对所提出的多模式折纸探针进行验证。