Monitor single-cell dynamics using optically computed phase microscopy in correlation with fluorescence characterization of intracellular properties

使用光学计算相位显微镜监测单细胞动力学与细胞内特性的荧光表征相关

• 批准号: 10589414
• 负责人: Xuan Liu
• 金额: $ 44.59万
• 依托单位: NEW JERSEY INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10589414
• 关键词: 3-Dimensional; Address; Adhesions; Adhesives; Affect; Architecture; Arthritis; Atherosclerosis; Binding; Biomedical Research; Cell Adhesion; Cell Survival; Cells; Characteristics; Clinical Research; Collaborations; Complex; Contrast Media; Data; Data Analyses; Detection; Development; Disease; Doxorubicin; Early Diagnosis; Exposure to; Extracellular Matrix; Fluorescence; Fluorescence Microscopy; Functional disorder; Funding; Glutathione Disulfide; Hela Cells; Image; Imaging Techniques; Imaging technology; Interdisciplinary Study; Interferometry; Investigation; Knowledge; Label; Lateral; Lead; Light; Malignant Neoplasms; Measurement; Measures; Mechanics; Methods; Microscopic; Microscopy; Mitochondria; Monitor; Motion; Multimodal Imaging; Optics; Organelles; Osteoporosis; Patients; Pharmaceutical Preparations; Phase; Play; Positioning Attribute; Process; Proliferating; Property; Protocols documentation; Research; Research Assistant; Research Personnel; Research Project Grants; Resolution; Role; Semantics; Signal Transduction; Structure; Time; Training; Trypsin; Validation; biomaterial development; career; cell behavior; cell preparation; cellular imaging; chemotherapy; data acquisition; deep neural network; experimental study; fundamental research; high throughput technology; image archival system; image processing; imaging capabilities; imaging platform; microscopic imaging; migration; nanoscale; next generation; optical imaging; photon-counting detector; preclinical study; quantitative imaging; technological innovation; technology development; undergraduate research; undergraduate student

Project Summary The objective of this study is to develop a multi-modality imaging platform that integrates complex phase microscopy (CPM) based on optical computation and fluorescence microscopy (FM). We will use the imaging platform to correlate the sub-cellular dynamic motion and intracellular properties to study cell adhesion which plays an important role in many aspects of cell behavior. This study is significant for fundamental research, preclinical and clinical study, and technology development. By simultaneously imaging cell motion and organelle properties, CPM-FM enables the investigation of cell dynamics in correlation biomolecular characteristics. CPM-FM investigation of cell adhesion will result in better understanding of pathophysiology of different diseases, open the door to new methods for disease treatment and early diagnosis, lead to the development biomaterials that need specific interaction with cells, and benefit patients and clinicians. This study will also establish the feasibility of CPM as an imaging technology that allows non-invasive, and continuous monitoring of cell activities without contrast agents. We will achieve the objective of this study through developing the CPM imaging platform (Aim 1), investigating high-throughput single-cell dynamic imaging using CPM (Aim 2) and investigating the correlation between adhesive properties using CPM and cell viability using fluorescence microscopy (Aim 3). This project will provide a unique platform for undergraduate students to conduct research. Undergraduate research assistants will play major roles in cell imaging, image processing, and data analysis. Undergraduate research assistants participating in this study are exposed to multi-disciplinary research that will prepare them for a career in the fields of biomedicine.

项目摘要 这项研究的目的是开发一个多模式成像平台，该平台整合复杂阶段 基于光学计算和荧光显微镜（FM）的显微镜（CPM）。我们将使用成像平台 将亚细胞动态运动和细胞内特性与研究细胞粘附相关联，该细胞粘附起着重要作用 在细胞行为的许多方面。这项研究对于基础研究，临床前和临床研究以及 技术发展。通过同时成像细胞运动和细胞器特性，CPM-FM可以实现 相关生物分子特征中细胞动力学的研究。 CPM-FM对细胞粘附的研究将导致 在更好地了解不同疾病的病理生理学时，为疾病治疗方法的新方法打开了 早期诊断，导致需要与细胞特定相互作用的开发生物材料，并使患者受益 临床医生。这项研究还将确定CPM作为允许无创和的成像技术的可行性，并且 连续监测没有对比剂的细胞活动。我们将通过 开发CPM成像平台（AIM 1），使用CPM研究高通量单细胞动态成像 （AIM 2）并使用CPM和使用荧光的细胞活力研究了粘合性能之间的相关性 显微镜（AIM 3）。该项目将为大学生进行研究提供一个独特的平台。 本科研究助理将在细胞成像，图像处理和数据分析中扮演重要角色。本科生 参加这项研究的研究助理受到了多学科研究，这将使他们为职业做好准备 在生物医学领域。