Oxygen imaging by phosphorescence quenching

磷光猝灭氧气成像

• 批准号: 10653893
• 负责人: SERGEI VINOGRADOV
• 金额: $ 30.07万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10653893
• 关键词: 3-Dimensional; Adopted; Adoption; Area; Benchmarking; Bioenergetics; Biology; Blood Transfusion; Cell Culture Techniques; Cellular Metabolic Process; Chemistry; Clinic; Clinical; Collaborations; Communities; Computer software; Consultations; Consumption; Data Analyses; Data Collection; Disease; Fiber Optics; Future; Grant; Health; Heart; Hematopoiesis; Image; Immunologics; Internet; Journals; Knowledge; Laboratories; Link; Measurement; Measures; Metabolism; Methods; Microscopic; Microscopy; Monitor; Names; National Institute of Neurological Disorders and Stroke; Nature; Neurosciences; Neurosciences Research; Ophthalmology; Oxygen; Oxygen saturation measurement; PUVA Photochemotherapy; Paper; Pathway interactions; Pennsylvania; Performance; Physiological; Play; Productivity; Protocols documentation; Publications; Radiation therapy; Recording of previous events; Research; Research Personnel; Resolution; Resources; Role; Running; Scanning; Site; Stroke; System; Techniques; Technology; Temperature; Time; Tissue Engineering; Tissue Therapy; Tissues; Training; Trauma; Tumor Biology; United States National Institutes of Health; Universities; Variant; Visit; Work; base; biological systems; biomedical imaging; bone engineering; cancer therapy; commercialization; data acquisition; document outlines; experience; experimental study; instrument; interest; large scale production; luminescence; manufacturing scale-up; medical attention; medical schools; method development; minimally invasive; open source; optical fiber; phosphorescence; pre-clinical; programs; quantitative imaging; scale up; software development; stem cell biology; symposium; tissue oxygenation; tissue regeneration; tomography; tumor immunology; two-photon; web site

A Resource for Oxygen Imaging by Phosphorescence Quenching Project Summary Real-time, minimally invasive and spatially resolved measurements of tissue oxygenation have the potential to transform our understanding of many clinical problems, including those in tumor biology, management of stroke, ophthalmology, tissue regeneration, to name a few. Over the years our laboratory has developed a minimally invasive method for dynamic imaging of oxygen in biological systems, known as phosphorescence quenching oximetry. Recently, the method has been expanded by two-photon phosphorescence lifetime microscopy (2PLM), enabling dynamic imaging of oxygen gradients in tissues in 3D with micron-scale resolution, opening new horizons for research in neuroscience, stem cell biology, cancer immunology, tissue engineering and several other areas. Even more recently, the combination of phosphorescence with Cherenkov-Excited Luminescence Scanned Imaging (CELSI) enabled tomography of oxygen in 3D in pre-clinical setting in tissues undergoing radiation therapy. At the same time, phosphorescence-based oximetry is increasingly drawing attention from the medical community for its potential to directly evaluate physiologic status of tissue under trauma, optimize efficacy of blood transfusion as well as a marker in monitoring progress of photodynamic and radiation therapies. These and other applications have set the stage for broad dissemination of the phosphorescence technology across different biomedical fields. At the heart of the phosphorescence quenching method are special oxygen probes, developed and continuously optimized in our laboratory. The synthesis of these probes, including the newest and the most potent probe Oxyphor 2P, is not simple, and standard commercialization pathways are presently not feasible. Here we propose to establish a U24 Resource that would allow us to sustain synthesis of phosphorescent probes, making them available to a broad range of biomedical researchers across different fields. Simultaneously, we will generate software for measuring/imaging oxygen by phosphorescence lifetime and will establish a center to provide consultations and training of new users interested in the method. The work will be performed at two closely collaborating sites: the University of Pennsylvania (probe chemistry, software development) and Martinos Center for Biomedical Imaging at the MGH (software development, user training). Our laboratories have long history of productive collaboration as well as multiple collaborations and contacts with researchers interested in oxygen. These collaborations along with the past experience of running a neuroscience research resource will help us to establish an effective program, making the phosphorescence- based oximetry accessible to a broad user base.

通过磷光猝灭进行氧成像的资源 项目摘要 实时，微创和空间分辨的组织氧合测量值 改变我们对许多临床问题的理解，包括肿瘤生物学的理解， 中风，眼科，组织再生的管理。多年来，我们的实验室有 开发了一种微创方法，用于在生物系统中的氧气成像，称为 磷光淬灭血氧饱和度。最近，该方法已通过两光子扩展 磷光寿命显微镜（2PLM），使3D组织中氧梯度的动态成像 通过微米级分辨率，为神经科学，干细胞生物学，癌症研究开辟了新的视野 免疫学，组织工程和其他几个领域。甚至最近， 用Cherenkov激发发光扫描成像（CELSI）的磷光功能使整洁 在接受放射疗法的组织中临床前环境中的3D中的氧气。同时， 基于磷光的血氧仪越来越多地吸引医学界的注意力 要直接评估创伤下组织的生理状况，请优化输血的功效 监测光动力和辐射疗法进度的标记。这些和其他申请有 为在不同的生物医学领域跨越磷光技术的广泛传播奠定了基础。 磷光猝灭方法的核心是特殊的氧探针，开发了， 在我们的实验室中不断优化。这些探针的综合，包括最新和最新的探针 有效的探针氧2p并不简单，并且标准的商业化途径目前不可行。 在这里，我们建议建立一个U24资源，以使我们能够维持磷光的合成 探针，使其可用于各个领域的各种生物医学研究人员。 同时，我们将生成用于通过磷光寿命测量/成像氧的软件，并且将 建立一个中心，以提供对该方法感兴趣的新用户的咨询和培训。工作将是 在两个紧密协作的网站上表演：宾夕法尼亚大学（探针化学，软件 开发）和MGH（软件开发，用户培训）的生物医学成像中心。 我们的实验室拥有悠久的生产协作历史以及多次合作和联系 与氧气感兴趣的研究人员。这些合作以及过去的经验 神经科学研究资源将帮助我们建立有效的计划，使磷光 - 基于大量用户群可以访问的血氧仪。

项目成果

Impact of sodium glucose linked cotransporter-2 inhibition on renal microvascular oxygen tension in a rodent model of diabetes mellitus.

• DOI: 10.14814/phy2.14890
• 发表时间: 2021-06
• 期刊: Physiological reports
• 影响因子: 2.5
• 作者: Hare GMT;Zhang Y;Chin K;Thai K;Jacobs E;Cazorla-Bak MP;Nghiem L;Wilson DF;Vinogradov SA;Connelly KA;Mazer CD;Evans RG;Gilbert RE
• 通讯作者: Gilbert RE

Measurement of cerebral oxygen pressure in living mice by two-photon phosphorescence lifetime microscopy.

• DOI: 10.1016/j.xpro.2022.101370
• 发表时间: 2022-06-17
• 期刊: STAR PROTOCOLS
• 作者: Erlebach, Eva;Ravotto, Luca;Wyss, Matthias T.;Condrau, Jacqueline;Troxler, Thomas;Vinogradov, Sergei A.;Weber, Bruno
• 通讯作者: Weber, Bruno