TR&D Project 2: Tissue and cellular elemental distribution, and image correlation

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• 批准号: 10652607
• 负责人: Chris Johnson Jacobsen
• 金额: $ 23.13万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10652607
• 关键词: 3-Dimensional; Ablation; Area; Automobile Driving; Biological Sciences; Brain; Cells; Chemicals; Code; Communities; Computer software; Dedications; Department of Energy; Development; Dyes; Elements; Fluorescence; Fluorescence Microscopy; Funding; Homeostasis; Image; Image Analysis; Inductively Coupled Plasma Mass Spectrometry; Label; Laboratories; Lasers; Length; Light; Light Microscope; Machine Learning; Maps; Metabolic; Metals; Methodology; Methods; Microscope; Microscopy; Modality; Modeling; Organ; Pathology; Peer Review; Photons; Preparation; Production; Proteins; Pythons; Quantitative Microscopy; Radiation induced damage; Reproduction; Research Personnel; Resistance; Resolution; Resources; Roentgen Rays; Sampling; Scanning; Schedule; Self Correction; Signal Transduction; Slice; Source; Specimen; Structure; Synchrotrons; System; TensorFlow; Thick; Three-Dimensional Imaging; Time; Tissues; Trace Elements; United States National Institutes of Health; X ray microscopy; X-Ray Tomography; absorption; beamline; cellular imaging; chemical standard; computer program; confocal imaging; cost; cryogenics; experience; fluorescence imaging; image visualization; imaging modality; instrument; light microscopy; meter; nanoscale; novel strategies; open source; open source tool; operation; preservation; programs; prototype; quantitative imaging; reconstruction; spectroscopic imaging; supercomputer; technology research and development; tomography; tool; transmission process

PROJECT SUMMARY - TR&D PROJECT 2 Tissue and Cellular Elemental Distribution, and Image Correlation This Technology Research and Development Project will develop Scanning X-Ray Fluorescence Microscopy (SFXM) for quantitative views of trace element distributions in cells and tissues. It will use cryogenic specimen preparation and imaging conditions as a gold standard for chemical and structural preservation as well as radiation damage resistance. We will develop a cryo SXFM capability with a hundredfold increase in accessible specimen area for beamline 8-BM of the Advanced Photon Source (APS) at Argonne National Laboratory, and provide support for its operation. This will double the accessible experimental time in cryo SXFM at the APS, divided between our BTRR and other biomedical researchers via no-cost, peer-reviewed General User Proposals to the APS. We will also introduce cryo specimen cross-compatibility with the nanoscale SFXM capabilities of the NIH- purchased Bionanoprobe at the APS, laser-ablation inductively coupled plasma mass spectrometry (LA-ICP- MS; TR&D 1) at Northwestern, photoacoustic microscopy (PAM; TR&D 2) at Northwestern, and correlative cryogenic confocal light microscope (C3LM) at the APS. We will develop and make available PyElements, a software platform to provide an integrated and cross- registered view of quantitative images from all of these modalities. We will incorporate into PyElements a numerical optimization approach to correcting for self-absorption of x-ray fluorescence in thicker specimens, so that one can obtain quantitative information even from thicker specimens in 3D. All of these developments will take place in the context of our Driving Biomedical Projects, with large-area cryo SXFM being of particular importance to Theme A (Metal homeostasis or dysregulation in brain function), Theme C (Metal fluxes controlling reproduction and development), and Theme D (Metal imbalances in metabolic pathology).

项目摘要-TR＆D项目2 组织和细胞元素分布以及图像相关性 该技术研发项目将开发扫描X射线荧光显微镜 （SFXM）用于细胞和组织中微量元素分布的定量观点。它将使用低温标本 制备和成像条件是化学和结构保存的黄金标准以及 辐射损伤阻力。我们将开发一个冷冻SXFM功能，可访问增加一百倍 Argonne国家实验室的高级光子源（APS）的光束线8-BM的样品区域， 为其操作提供支持。这将使APS中的Cryo SXFM中可访问的实验时间增加一倍， 通过无成本，同行评审的普通用户在我们的BTRR和其他生物医学研究人员之间进行划分 对APS的建议。 我们还将使用NIH-的纳米级SFXM功能引入冷冻标本交叉兼容性 在APS上购买了bionanoprobe，激光燃烧电感耦合等离子体质谱法（LA-ICP- 多发性硬化症; TR＆D 1）在西北，光声显微镜（PAM; TR＆D 2）在西北地区和相关性 APS处的低温共聚焦显微镜（C3LM）。 我们将开发和提供Posis Pyelements，这是一个软件平台，可提供集成和交叉的 来自所有这些模式的定量图像的注册视图。我们将纳入页而 数值优化方法是校正较厚标本中X射线荧光自我吸收的方法，因此 即使从3D中的较厚标本中也可以获得定量信息。所有这些发展都将 发生在我们驾驶生物医学项目的背景下，大区域冷冻SXFM特别 主题A的重要性（金属稳态或大脑功能中的失调），主题C（金属通量控制 繁殖和发展）和主题D（代谢病理中的金属失衡）。