Oral microbiome establishment and development of Latinx Children at the US-Mexico border

美墨边境拉丁裔儿童口腔微生物群的建立和发展

• 批准号: 10644580
• 负责人: Yan Wang
• 金额: $ 13.92万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10644580
• 关键词: 16S ribosomal RNA sequencing; Adult; Age; Algorithms; Allergic Disease; Antibiotics; Bacteria; Birth; Breast Feeding; Cardiovascular Diseases; Caregivers; Child; Child Health; Childhood; Chlorhexidine; Chronic; Collaborations; Complex; Control Groups; Counseling; County; Data; Dental caries; Dentistry; Development; Diet; Disease; Early Intervention; Ecosystem; Environment; Foundations; Freezing; Funding; Future; Goals; Health; Healthcare; High Prevalence; Hispanic; Hispanic Populations; Horizontal Disease Transmission; Human; Infant; Intervention; Knowledge; Latinx; Life; Literature; Los Angeles; Measures; Mentored Research Scientist Development Award; Mentors; Mentorship; Mexico; Microbe; Modeling; Modernization; Mothers; Mouth Diseases; Neonatal; Newborn Infant; Oral; Oral Characters; Oral cavity; Oral health; Oral mucous membrane structure; Outcome; Parents; Participant; Pattern; Pediatric Dentistry; Placenta; Placental microbiome; Play; Population; Postpartum Period; Pregnancy Complications; Preschool Child; Prevention program; Preventive; Proxy; Public Health; Qualifying; Regimen; Research; Research Activity; Research Personnel; Role; Sampling; Schedule; School Dentistry; Second Pregnancy Trimester; Statistical Data Interpretation; Surface; Surveys; Techniques; Therapeutic; Time; Tooth Diseases; Tooth structure; Training; United States; United States National Institutes of Health; Virus; Visit; Weight Gain; Work; Youth; autism spectrum disorder; biomarker discovery; biomarker identification; career; career development; deciduous tooth; disorder risk; early childhood; ethnic disparity; evidence base; experience; fungus; health disparity; health disparity populations; high risk; immune function; innovation; insight; kindergarten; medical schools; microbial; microbiome; microbiome composition; microbiome research; microbiota; microorganism; oral microbial community; oral microbiome; peer; personalized therapeutic; prenatal; prevent; racial disparity; randomized, clinical trials; research and development; risk prediction; saliva sample; transmission process; treatment group

PROJECT SUMMARY/ABSTRACT Early life interactions between the microbiome and its human host are responsible for an array of immune functions and future health outcomes. It is essential to study this period during early life when the oral microbiome changes from highly dynamic infant microbiota towards a more stable adult-like microbiota. The oral microbiome plays an essential role in oral and systemic health. As such, the study of the oral microbiome may serve as a harbinger for children’s health. Understanding the establishment and early development of the oral microbiome, and its association with the early childhood caries (ECC), may provide innovative solutions for personalized, predictive, and preventive pediatric dentistry. This Mentored Research Development Award K01 study seeks to understand the dynamic changes of the oral microbiome during the first three years of life among children who were born along the United States (US)-Mexico border. These children experience a high risk of dental caries and other oral diseases; therefore, providing a complex environment for the microbiome. The study aims are: (1) to understand the establishment of newborn oral microbiome among Latinx/Hispanic children living along the US-Mexico border by using salivary samples at prenatal (from mother), 4-, 9-, 12-, 24-, 36-months to characterize their oral microbiome establishment from birth to a full set of twenty primary teeth; (2) to compare children’s oral microbiome composition between control and treatment groups (mothers received therapeutic three-month regimen of chlorhexidine mouth rinse to reduce the bacteria that causes tooth decay when their children were between the ages of four to six months) while both groups received oral health counseling; and (3) to develop statistical expertise in modeling the changes of oral microbiota and their association with ECC controlling for confounding factors. This proposed research will use three recently developed Bayesian-based ML algorithms to model the temporal patterns of the oral microbiome and evaluate their ability to predict ECC. The K01 will support Dr. Yan Wang to develop expertise in: (1) oral microbiome development in early childhood, (2) statistical analysis for oral microbiome data, and (3) biomarker discoveries using saliva samples. To achieve these training goals and research activities, Dr. Wang has assembled a highly qualified mentorship team with substantial experience in mentoring early-career investigators and with expertise in oral microbiome research among children. Dr. Wang’s primary mentors at UCLA, Dr. Grace Aldrovandi (School of Medicine) and Dr. David Wong (School of Dentistry), are highly experienced NIH-funded investigators. Co-mentor Dr. Francisco Ramos-Gomez (School of Dentistry) is a leading expert in pediatric oral health, especially among Latinx/Hispanic children. The proposed research and training aims will build strong research capacities and collaborations for Dr. Yan Wang. In addition, this work will advance Dr. Yan Wang’s research career in analytical and translational oral microbiome research.

项目摘要/摘要 微生物组及其人类宿主之间的早期生活互动是造成一系列免疫力的原因 功能和未来的健康成果。在口腔时期研究这一时期至关重要 微生物组从高度动态的婴儿微生物群变为更稳定的成人微生物群。这 口服微生物组在口腔和系统健康中起着至关重要的作用。因此，口服微生物组的研究 可以作为儿童健康的预兆。了解建立和早期发展 口服微生物组及其与幼儿相关的携带者（ECC），可以为 个性化，预测和预防性小儿牙科。这个指导的研究发展奖K01 研究试图了解生命的头三年中口腔微生物组的动态变化 在美国沿线（美国） - 梅克西科边界的孩子中。这些孩子很高 龋齿和其他口腔疾病的风险；因此，为微生物组提供复杂的环境。 该研究的目的是：（1）了解拉丁语/西班牙裔新生儿口腔微生物组的建立 通过在产前（来自母亲）的唾液样本（4-，9-，9-，12-，12-，24-， 36个月以表征其口服微生物组的建立，从出生到全套二十颗牙齿； （2）比较对照组和治疗组（母亲）之间儿童的口服微生物组组成 接受了氯己定嘴的三个月热疗法，以减少引起的细菌 当他们的孩子年龄在四到六个月之间时，蛀牙），两组接受口头 健康咨询； （3）开发统计专业知识，以建模口服微生物群的变化及 与ECC控制混杂因素的关联。这项拟议的研究将最近使用三个 开发了基于贝叶斯的ML算法，以建模口服微生物组的临时模式并评估 他们预测ECC的能力。 K01将支持Yan Wang博士开发专业知识：（1）口服微生物组 幼儿期的发展，（2）口服微生物组数据的统计分析和（3）生物标志物发现 使用唾液样品。为了实现这些培训目标和研究活动，Wang博士集会了 高素质的Mentalship团队，在心理早期研究人员以及与 儿童口腔微生物组研究方面的专业知识。王博士在加州大学洛杉矶分校的主要导师格蕾丝博士 Aldrovandi（医学院）和David Wong博士（牙科学院）是经验丰富的NIH资助 调查人员。弗朗西斯科·拉莫斯·戈梅斯（Francisco Ramos-Gomez）博士（牙科学院）是小儿口服的领先专家 健康，尤其是在拉丁裔/西班牙裔儿童中。拟议的研究和培训目标将建立强大 Yan Wang博士的研究能力和合作。此外，这项工作将推动Yan Wang博士的 分析和翻译口腔微生物组研究的研究职业。