Examining Associations between the Oral Microbiota, Neuroinflammation, and Binge Drinking in Adolescents

检查青少年口腔微生物群、神经炎症和酗酒之间的关联

• 批准号: 10679789
• 负责人: Brittney Browning
• 金额: $ 4.51万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10679789
• 关键词: 16S ribosomal RNA sequencing; 21 year old; Address; Adolescence; Adolescent; Adult; Affect; Age; Alcohol abuse; Alcohol consumption; Alcohols; Anterior; Area; Astrocytes; Brain; Brain region; Cells; Choline; Clinical; Clinical Trials; Data; Data Analyses; Data Collection; Development; Diagnosis; Diagnostic; Eligibility Determination; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Foundations; Funding; Gingiva; Goals; Human; Immune; Immune system; Individual; Inflammatory; Interleukin-1 beta; Interleukin-6; Intervention; Knowledge; Life; Magnetic Resonance Spectroscopy; Maintenance; Measures; Mediating; Meta-Analysis; Methods; Microbe; Microglia; Neurobiology; Neuroglia; Neurosciences; Oral; Participant; Peripheral; Physiological Processes; Play; Population; Positioning Attribute; Postdoctoral Fellow; Predisposition; Prevention strategy; Protons; Research; Risk Factors; Role; Saliva; Sampling; Secure; Substance Use Disorder; TNF gene; Training; United States National Institutes of Health; Youth; adolescent binge drinking; alcohol effect; alcohol use disorder; alcohol use initiation; binge drinker; binge drinking; career development; cingulate cortex; cohort; comparison control; critical period; cytokine; disorder prevention; early alcohol use; experience; gut microbiota; hands on research; in vivo; inflammatory marker; interest; microbial; microbial composition; microbiome; microbiome alteration; microbiome research; microbiota; myoinositol; neural; neurodevelopment; neuroimaging; neuroinflammation; novel; oral microbial community; parent grant; response biomarker; skills; training opportunity; translational scientist; translational study; treatment response; underage drinking

PROJECT SUMMARY/ABSTRACT The proposed application responds to the need for a better understanding of the microbiome and neuroinflammation in adolescent alcohol users. Early initiation of alcohol use, and binge drinking in particular, is considered one of the most important risk factors in the later development of alcohol and substance use disorders, but the neurobiological changes that may account for this increased vulnerability are not well understood. Alcohol-induced microbiome alterations at an early age may lead to neurometabolite alterations and in-turn affect the escalation of alcohol use. Consequently, the microbiome may be a novel area of research that may aid in adolescent AUD prevention and intervention efforts by providing new targets for treatment, and/or diagnostic and therapeutic response biomarkers. The present study therefore aims to investigate associations between the microbiota and neuroinflammation in the context of adolescent binge drinking. Specifically, the study will rely on analysis of oral microbiota 16S rRNA sequencing and magnetic resonance spectroscopy (MRS) data, in order to determine if binge drinking adolescents have higher levels of pro-inflammatory microbes and MRS markers of neuroinflammation. Together these data, regardless of if they are line with our hypotheses, will provide a foundation for neuroscience and microbiome informed targets and strategies for prevention and intervention efforts for problematic alcohol use. The research proposed in this F31 application will serve as essential hands-on training to promote Brittney's career development in adolescent alcohol use disorder neurobiology, neuroimaging, and the microbiome, and it will result in data on which to build an NIH F32 application.

项目摘要/摘要 拟议的应用程序响应需要更好地了解微生物组和 青春期酒精使用者的神经炎症。早期开始使用酒精，尤其是饮酒，是 被认为是饮酒和物质使用的后来开发中最重要的危险因素之一 疾病，但可能解释这种增加脆弱性的神经生物学变化不好 理解。酒精诱导的微生物组在幼年时的改变可能会导致神经代谢物的改变，并且 往返会影响饮酒的升级。因此，微生物组可能是一个新的研究领域， 可以通过提供新的治疗目标和/或 诊断和治疗反应生物标志物。因此，本研究旨在调查关联 在青春期暴饮暴食的背景下，微生物群和神经炎症之间。具体而言，该研究 将依赖于口服微生物群16S rRNA测序和磁共振光谱（MRS）数据的分析， 为了确定暴饮暴食青少年是否具有较高的促炎性微生物和MRS 神经炎症的标记。这些数据（无论它们是否与我们的假设保持一致，都将加在一起，都会 为神经科学和微生物组知情目标和预防策略提供基础 干预饮酒的干预工作。该F31申请中提出的研究将作为 基本的动手培训，以促进Brittney在青少年饮酒障碍中的职业发展 神经生物学，神经影像学和微生物组，它将导致构建NIH F32的数据 应用。