Animal, Behavior and Tissue Core

动物、行为和组织核心

基本信息

批准号：
10496282
负责人：
EBENEZER N YAMOAH
金额：
$ 48.63万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10496282
关键词：
Action Potentials Address Affect Age Distribution Aging Animal Model Animals Auditory Auditory area Auditory system Backcrossings Behavior Binaural Biological Markers Brain Stem Breeding Clinical Cochlear nucleus Code Complex Computer Analysis Cryopreservation Demyelinations Detection Development Disasters Disease Disease Outbreaks Exhibits FDA approved Functional disorder Gene Targeting Generations Genes Genetic Genetically Engineered Mouse Genetically Modified Animals Genotype Goals Human Impaired cognition Individual Inhibitory Synapse Joints Knock-in Mouse Laboratories Left Link Maps Mental Depression Modeling Molecular Mus Nerve Degeneration Neural Pathways Neurons Outcome Persons Pharmaceutical Preparations Phase Pitch Perception Presbycusis Program Research Project Grants Property Proteomics Public Health Quality of life Research Resources Rodent Role Sensorineural Hearing Loss Services Social isolation Speech Intelligibility Speed Statistical Data Interpretation Stimulus Stretching Synapses Synaptic plasticity Testing Therapeutic Tissues Transgenic Mice United States National Institutes of Health age related aged aging auditory system aging population animal facility auditory pathway calbindin calretinin combat cost effective design diphtheria toxin receptor excitotoxicity experimental study genetic approach handbook inducible Cre innovation interest mouse model neural neural hearing loss neural network neuron loss neurosensory novel optogenetics otoacoustic emission programs progressive hearing loss response senescence sound tool

项目摘要

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially or are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects, Core C and Core D within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the structural and functional remodeling in the auditory neural pathways. The proposed PPG will require the use of gene-targeted mouse models to study the mechanistic underpinning in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects, Core C and Core D. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.

抽象的老年和基因工程小鼠模型的开发和利用对于理解至关重要特定基因在年龄相关性听力损失（ARHL）中的作用。我们将需要菌株特异性老化（衰老）无法商业化或在 NIH 的 NIA 支持的设施中生产的小鼠， http://www.nia.nih.gov/research/dab/ged-rodent-colonies-handbook。所有项目，核心 C 和核心 D 拟议的 PPG 广泛利用这些老年小鼠，并利用多种遗传方法来研究听觉神经通路的结构和功能重塑。拟议的 PPG 将需要使用基因靶向小鼠模型来研究衰老听觉系统的机制基础。小鼠核心的目的是为品系特异性老龄小鼠的建立提供服务群体以及基因靶向和转基因小鼠的产生和管理。方正老鼠将在 UNR 的动物设施中进行鉴定、饲养和饲养。此外，还将培育数种小鼠品系老化并提供给所有三个项目（Core C 和 Core D）中提议的实验。该 Core 也将负责维护和分配鼠标线到每个单独的项目。这将提供最具有成本效益的共享和利用鼠标模型。此外，核心还将冷冻保存关键的以防意外丢失线路。最后，核心将成为其他感兴趣的实验室的资源 ARHL小鼠模型。