Mouse Genetics Core

小鼠遗传学核心

基本信息

批准号：
9151169
负责人：
EBENEZER N YAMOAH
金额：
$ 26.22万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2021-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9151169
关键词：
Address Age Aging Animal Model Auditory Auditory system Backcrossings Blinded Breeding Code Data Development Disasters Disease Outbreaks Doxycycline Endolymph Ensure Epithelium Facility Controls Gene Targeting Generations Genes Genetic Genetically Engineered Mouse Genetically Modified Animals Genotype Goals Hair Cells Human Resources In Vitro Individual Laboratories Labyrinth Mediating Modeling Molecular Mus Mutant Strains Mice Nerve Degeneration Neural Pathways Neurons Outcome Perilymph Presbycusis Process Program Research Project Grants Proteins Research Resources Rodent Role Sensory Sensory Hair Services Site Sum Synapses Testing Tetanus Helper Peptide Therapeutic Tight Junctions Transcript Transgenic Mice Transgenic Model United States National Institutes of Health abstracting age related aged aging gene animal facility combat cost effective design genetic approach handbook hearing impairment in vivo innovation interest meetings mouse model mutant novel occludin optogenetics programs relating to nervous system research study senescence spiral ganglion success

项目摘要

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially and are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects and Core C within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the role of several genes in the inner ear and auditory neural pathways. Indeed, the proposed PPG will require the use of diseased models that result in hearing loss in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects and Core C. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.

抽象的老年和基因工程小鼠模型的开发和利用对于理解至关重要特定基因在年龄相关性听力损失（ARHL）中的作用。我们将需要菌株特异性老化（衰老）小鼠无法在商业上买到，并且是在 NIH 的 NIA 支持的设施中生产的， http://www.nia.nih.gov/research/dab/ged-rodent-colonies-handbook。内的所有项目和核心 C 提出的 PPG 广泛利用这些老年小鼠，并利用多种遗传方法进行研究几个基因在内耳和听觉神经通路中的作用。事实上，拟议的 PPG 将需要使用患病模型会导致老化听觉系统听力损失。鼠标的目的核心是提供品系特异性老年小鼠群体的建立及代代代种服务基因靶向和转基因小鼠的管理。创始人小鼠将在一个 UNR 的动物设施。此外，还将培育和老化数种小鼠品系并提供用于实验在所有三个项目和核心 C 中提出。核心还将负责维护和分发鼠标线到每个单独的项目。这将提供最具成本效益的共享和利用鼠标模型。此外，Core 还将冷冻保存关键线路，以防意外丢失。最后，该核心将为其他对 ARHL 小鼠模型感兴趣的实验室提供资源。