Mechanism of Cell Competition

细胞竞争机制

基本信息

批准号：
9360551
负责人：
Nicholas E Baker
金额：
$ 20.88万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-30 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9360551
关键词：
Adult Affect Apoptosis Biological Assay CCAAT-Enhancer-Binding Proteins Cancerous Candidate Disease Gene Cells Chimera organism Cornea DNA Binding Development Drosophila genus Embryo Eye Eye Development Family Gene Mutation Gene Proteins Genes Genetic Genetic Epistasis Genetic Transcription Genomics Genotype Goals Growth High-Throughput Nucleotide Sequencing Homologous Gene Homologous Protein Human Injury Keratoplasty Mediating Messenger RNA Methods Molecular Mosaicism Mus Mutation Normal Cell Normal tissue morphology Organ Outcome Pathway interactions Phenotype Population Process Proteins Regulation Rejuvenation Research Ribosomal Proteins Role Stem cells Testing Tissues Translations Transplantation Vision bZIP Protein cell growth cell type defined contribution experimental study genome-wide imaginal disc in vivo knock-down mutant novel overexpression prevent public health relevance repaired response stem cell therapy success tissue mosaicism transcription factor tumor tumor progression

项目摘要

DESCRIPTION (provided by applicant): The long term goals of this research are to understand the factors that underlie the survival or elimination of particular cells within tissues and how they may affect the success of transplantation and stem cell treatments such as applied to the cornea. The specific goal is to elucidate the contribution of a particular transcription factor recently found to have a novel effect on tissue growth. This protein delays the growth of cells with alterations in the protein synthetic machinery and enables their elimination from tissues. Genetic epistasis experiments using mutant fruit flies will be employed to define the contribution of a family of related bZIP transcription factors to the growth delay an competitive loss of cells, in part employing effects on eye size as an assay. The genome-wide contribution of selected bZIP transcription factors to gene transcription will be determined using high- throughput sequencing methods both in normal tissues and in tissues with alterations in protein synthetic machinery, with the goal of understanding the regulatory processes that control the rate of growth and the competition between cells that occurs in chimeras and perhaps in normal or cancerous tissues.

描述（由申请人提供）：这项研究的长期目标是了解组织内特定细胞存活或消除的因素，以及它们如何影响移植和干细胞治疗（例如应用于角膜）的成功。具体目标是阐明最近发现的对组织生长具有新作用的特定转录因子的贡献，该蛋白质通过改变蛋白质合成机制来延迟细胞的生长，并利用突变体的遗传上位实验使其能够从组织中消除。果蝇将用于定义相关 bZIP 转录因子家族对细胞生长延迟和竞争性损失的贡献，部分采用对眼睛大小的影响作为测定。选定的 bZIP 转录因子对基因转录的全基因组贡献。可以在正常组织和蛋白质合成机制发生改变的组织中使用高通量测序方法来确定，目的是了解控制嵌合体中以及可能在正常或正常组织中发生的细胞之间的生长速率和竞争的调节过程。癌组织。