Advanced Confocal Microscope in a multi-user facility

多用户设施中的先进共焦显微镜

• 批准号: 9274630
• 负责人: Nicholas E Baker
• 金额: $ 59.04万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274630
• 关键词: Aging; Apoptosis; Autophagocytosis; Biomedical Research; Brain; Confocal Microscopy; Contract Services; Developmental Biology; Diabetes Mellitus; Drosophila genus; Enhancement Technology; Funding; Gap Junctions; Grant; Hippocampus (Brain); Human; Image; Inflammatory; Interdisciplinary Study; Investigation; Lasers; Medicine; Messenger RNA; Microscope; Microscopy; Minor; Mitochondria; Neoplasm Metastasis; Neurons; Regulation; Research Personnel; Resolution; Scanning; Speed; Technology; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Update; Vendor; bone; college; design; detector; developmental genetics; experimental study; flexibility; fluorophore; innovation; instrument; instrumentation; live cell imaging; repaired

PROJECT SUMMARY/ABSTRACT This application is a shared instrumentation grant from the Analytical Imaging Facility (AIF) at the Albert Einstein College of Medicine to acquire a new, advanced point-scanning confocal microscope. The AIF supports NIH-funded investigators by giving them access to state-of-the- art microscopy technologies that enhance cutting edge, collaborative, and multidisciplinary research. Currently, the AIF has several confocal microscopes available: two point-scanning confocals, a spinning disk confocal and a line scanning confocal. However, three out of the four existing confocal microscopes are reaching their end of usage due to aging, discontinuation of service contracts by the vendors, and difficulties obtaining parts needed for repair. The one remaining point-scanning confocal microscope will not be able to support the 70+ labs currently using confocal technology at Einstein. In order to continue to support the ongoing NIH funded biomedical research that relies on confocal microscopy, we need to replace the failing instruments with one new confocal microscope that can offer all the functions of our current microscopes, including regular multi-channel high resolution 3D imaging, live-cell imaging at fast frame rates, and flexibility in channel set-up to enable use of many different fluorophores. In addition, since the acquisition of our last confocal in 2007, there have been some major innovations in the field of confocal microscopy with regard to detectors, laser design and acquisition speed, and we believe that an update in technology will enable some of our NIH- funded researchers to conduct experiments that have previously been challenging to conduct. Overall, acquisition of this advanced instrument will have a high impact on the NIH-funded biomedical research at Einstein, including the following major user projects: studies of the developmental biology and genetics of Drosophila (Dr. Baker), studies of central inflammatory regulation of systemic aging in humans (Dr. Cai), studies of cancer metastasis, invasion and intravasation (Dr. Condeelis), mitochondrial function and mitophagy (Dr. Kitsis), the regulation of autophagy and programmed cell death in the context of diabetes (Dr. Pessin), mechanisms of mRNA regulation in primary hippocampal neurons and tissues (Dr. Singer) and investigation of gap junction structural dynamics in the brain and bone (Dr. Spray). There are also additional minor user projects and 70+ labs currently using confocal microscopy here at Einstein that will benefit from this technology.

项目摘要/摘要 该应用程序是分析成像设施（AIF）的共享仪器赠款 阿尔伯特·爱因斯坦医学院获得了新的高级点扫描共焦 显微镜。 AIF通过使他们获得最先进的调查员来支持NIH资助的调查员 艺术显微镜技术，可增强尖端，协作和多学科 研究。目前，AIF有几个共聚焦显微镜：两个点扫描 共焦，旋转磁盘共焦和线条扫描共聚焦。但是，四分之一 由于老化，停用 供应商的服务合同，以及获得维修所需零件的困难。一个 剩余的点扫描共聚焦显微镜将无法支持当前的70+实验室 在爱因斯坦使用共聚焦技术。为了继续支持正在进行的NIH资助 依赖共聚焦显微镜的生物医学研究，我们需要替换失败的 具有一个新的共聚焦显微镜的乐器，可以提供我们当前的所有功能 显微镜，包括常规的多通道高分辨率3D成像，快速的活细胞成像 帧速率和通道设置的灵活性，以实现许多不同的荧光团。在 此外，自从2007年收购我们的最后一次共焦以来，已经有一些专业 关于探测器，激光设计和共聚焦显微镜领域的创新 获取速度，我们相信技术的更新将使我们的一些NIH- 资助的研究人员进行了以前在进行挑战的实验。 总体而言，收购该高级工具将对NIH资助 爱因斯坦的生物医学研究，包括以下主要用户项目：研究 果蝇（Baker博士）的发育生物学和遗传学，中央炎症研究 调节人类全身衰老（CAI博士），癌症转移，侵袭和 侵入（Condeelis博士），线粒体功能和线粒体（Kitsis博士），调节 在糖尿病的背景下（Pessin博士）的自噬和程序性细胞死亡，机制 原发性海马神经元和组织（Singer博士）的mRNA调节以及研究 大脑和骨骼中的间隙连接结构动力学（Spray Dr.）。还有其他 当前在爱因斯坦使用共聚焦显微镜的次要用户项目和70多个实验室 从这项技术中受益。