Live-Cell Imaging Core

活细胞成像核心

• 批准号: 9146947
• 负责人: JAMES K WAHL
• 金额: $ 25.8万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9146947
• 关键词: Applications Grants; Behavior; Cells; Cloning Vectors; Collaborations; Communities; Consultations; Data; Dentistry; Energy Transfer; Equipment; Extramural Activities; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Funding; Generations; Goals; Grant; Image; Individual; Institutes; Institution; Life; Medical center; Microscopy; Molecular Cloning; Nebraska; Organelles; Phase; Physiological; Process; Proteins; Publications; Reagent; Research; Research Personnel; Research Project Grants; Resolution; Services; Signal Transduction; System; Techniques; Technology; Thinking; Time; Transplantation; Universities; abstracting; base; college; experience; expression vector; instrumentation; interest; live cell imaging; live cell microscopy; member; protein complex; research study; success

Abstract The Nebraska Center for Cellular Signaling (NCCS) Live-Cell Imaging Core provides a variety of support mechanisms to investigators within the NCCS and our partnering institutions through consultation services, reagents, and expertise in an array of microscopy techniques. The original goal of making advanced microscopy technologies readily available to the members of the NCCS continues to drive the success of the core and has been extended to support the larger research community. Since its formation at the beginning of Phase II, the Live-Cell Imaging Core has supported investigators through the generation of data for grant submissions and publications. The Live-Cell Imaging Core is composed of facilities with shared live-cell imaging capabilities as well as specialized features for unique applications. As part of Phase III funding of the NCCS, we are requesting funds to support the aims of the Live-Cell Imaging Core. Aim 1: Provide and maintain state-of-the-art live-cell microscopy equipment and offer technical support to investigators both within and outside the NCCS. Aim 2: Assist in the generation of image acquisition and analysis, allowing investigators to incorporate live-cell microscopy into their thinking and into their projects and increasing the likelihood grant proposals will be funded. Aim 3: Expand the capacity for collaboration between our Center and its partnering institutions. During Phase II, the Live-Cell Imaging Core established the technologies and the systems needed for advanced microscopy platforms. Together with strong institutional support, we propose to continue our momentum by implementing super-resolution microscopy capabilities for TIRF (Total Internal Reflection Fluorescence) microscopy and PALM (Photoactivated Localization Microscopy) within the Live-Cell Imaging Core. Addition of these technologies will greatly enhance the ability of NCCS investigators to compete for extramural funding. Long-term sustainability of the Live-Cell Imaging Core will be achieved by: 1) maintaining a strong base of well-funded users; 2) expansion of collaborations with users outside the NCCS; and 3) continuation of steady institutional support.

抽象的 内布拉斯加州的细胞信号传导中心（NCCS）活细胞成像核心通过咨询服务，试剂和一系列显微镜技术的专业知识为NCCS和我们的合作机构内的研究人员提供了多种支持机制。 NCC成员容易获得高级显微镜技术的最初目标继续推动核心的成功，并已扩展以支持更大的研究社区。自从第二阶段开始时成立，实时成像核心通过生成赠款提交和出版物的数据来支持研究人员。实时成像核心由具有共享的活细胞成像功能以及用于独特应用的专用功能组成。作为NCC的III阶段资金的一部分，我们要求资金支持Live-Cell Imaging Core的目标。 AIM 1：提供并维护最先进的活细胞显微镜设备，并为NCC内外的调查人员提供技术支持。 AIM 2：协助产生图像获取和分析，使研究人员能够将活细胞显微镜纳入他们的思维和项目中，并增加可能性的赠款建议。目标3：扩大我们中心与其合作机构之间的合作能力。在II阶段，活细胞成像核心建立了高级显微镜平台所需的技术和系统。加上强大的机构支持，我们建议通过在活细胞成像核中实现TIRF（总内反射荧光）显微镜和棕榈（光活化定位显微镜）的超分辨率显微镜功能来继续我们的动力。这些技术的增加将大大提高NCC调查人员争夺壁外资金的能力。通过以下方式，将实现活电池成像核心的长期可持续性：1）维持大量资金丰富的用户； 2）扩展与NCC之外用户的合作； 3）继续稳定的机构支持。