Core Grant for Vision Research

视觉研究核心资助

• 批准号: 8150782
• 负责人: Curtis R Brandt
• 金额: $ 60.2万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8150782
• 关键词: Animal Model; Area; Cancer Center Support Grant; Cells; Cultured Cells; Development; Disease; Eye; Felis catus; Funding; Gene Delivery; Gene Expression; Genes; Glaucoma; Grant; Health; Histology; Image; Image Analysis; In Vitro; Infectious Agent; Instruction; Methods; Microscopy; Molecular Biology; Ocular Physiology; Organ Culture Techniques; Oryctolagus cuniculus; Paper; Pathology; Peer Review; Pharmaceutical Preparations; Primates; Qualifying; Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Rodent; Technical Expertise; Technology; Tissues; Translational Research; Universities; Vision; Vision research; Visual system structure; Wisconsin; Work; imaging modality; improved; morphometry; novel therapeutic intervention; programs; ranpirnase; tool; vector

DESCRIPTION (provided by applicant): This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules; Gene Delivery/Quantitative Molecular Biology; Pathology and Imaging; and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.

描述（由申请人提供）：威斯康星大学视觉研究人员的这项P30核心赠款提出了三个模块；基因递送/定量分子生物学；病理和成像；以及动物模型和眼器官文化。有效地将基因传递到细胞的能力是治疗眼部疾病的强大新治疗方法，并且是必不可少的研究工具。基因输送模块将构建向量，准备这些材料的高滴水库存供研究人员使用，并协助研究人员使用基因递送方法。定量方法在分子生物学中的使用正在增长。特别是，通过RT-PCR对基因表达的定量以及使用阵列技术的基因表达变化的分析。基因输送/定量分子生物学模块将为这些领域提供专门的技术专业知识，并将增强目前使用这些技术将其添加到其研究计划中的研究人员的能力。随着对翻译研究的重视，病理分析和复杂的成像方法变得越来越重要。病理学和成像模块将提供组织切片和培养细胞的高级组织学，显微镜和图像分析以及形态计量学。新疗法的开发和提高我们对视觉系统功能的理解依赖于在体外进行的工作转移到动物模型中。动物模型和眼器器官培养模块将帮助研究人员使用动物模型（灵长类动物，猫，兔子和啮齿动物），实验性青光眼诱导，成像和眼生理学。这种专业知识将有助于核心用户的努力，以协助其传染性药物，药物，操纵和矢量/构造对啮齿动物，猫，兔子和灵长类动物的眼功能的影响。在上一个资金期间，共有28个合格的NEI补助金。截至提交日期，有188个同行评审的论文涉及使用核心。