Pathogenesis of Vaccinia virus keratitis

痘苗病毒性角膜炎的发病机制

• 批准号: 8445214
• 负责人: Curtis R Brandt
• 金额: $ 21.45万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8445214
• 关键词: Accounting; Animals; Attenuated; B-Lymphocytes; Biological; CD4 Positive T Lymphocytes; CD8-Positive T-Lymphocytes; Centers for Disease Control and Prevention (U.S.); Conjunctivitis; Cornea; Data; Disease; Disease Outbreaks; Exudate; Eye Infections; Future; Goals; Herpesvirus 1; Human; Immunology; Infection; Inflammatory; Keratitis; Life; Military Personnel; Modeling; Monkeypox; Mouse Strains; Mus; Oryctolagus cuniculus; Outcome; Paper; Pathogenesis; Pathology; Poxviridae; Publishing; Pus; Reagent; Regimen; Russia; Severity of illness; Simplexvirus; Smallpox; Smallpox Vaccine; Steroids; System; Testing; Therapeutic; Tissues; USSR; United States; Vaccination; Vaccines; Vaccinia; Vaccinia virus; Vaccinium; Viral; Virus; Virus Diseases; base; cytokine; emergency service responder; macrophage; mouse model; neutrophil; novel; prednisolone; response; weapons

Project Summary Although Smallpox no longer naturally circulates it remains a potential threat as a biological weapon. Currently, Smallpox is listed as the most important viral bioweapon by the United States. As a result, in 2001, the US resumed smallpox vaccinations for first responders (eg. ER personnel) and the military. The Smallpox vaccine, Vaccinia virus, is a live attenuated virus and adverse vaccine responses are common. In addition, outbreaks of animal pox viruses occasionally occur in humans. There is a possibility that infection of the eye with ensuing conjuncitivitis and keratitis, and perhaps blinding disease, can occur either following vaccination or zoonotic transfer . Virtually nothing is known about pathological mechanisms involved in Vaccinia ocular infections or other poxvirus ocular infections. Previously we developed a quantitative rabbit model of Vaccinia virus keratitis and we showed that the inclusion of topical steroids in therapy was potentially dangerous. In all groups that received prednisolone, keratitis rebounded within 3-4 days of halting the treatment and this was concomitant with the resumption of viral replication. Mouse models are much more suitable for studies on pathology and immunology of Vaccinia infections because of reagent availability. Therefore, the goal of this R21 application is to develop a novel quantitative mouse model of Vaccinia virus ocular infection.

项目摘要 尽管天花不再自然循环，但作为生物学，它仍然是潜在的威胁 武器。目前，天花被联合列为最重要的病毒生物武器 国家。结果，2001年，美国恢复了第一响应者的天花疫苗接种（例如 人员）和军队。天花疫苗，Vaccinia病毒，是一种活的减毒病毒， 不良疫苗反应很常见。此外，动物痘病毒的爆发 偶尔发生在人类中。随之而来的眼睛有可能感染 疫苗接种后可能发生结膜炎和角膜炎，甚至可能发生盲目疾病 或人畜共患转移。实际上，关于涉及的病理机制一无所知 疫苗眼感染或其他痘病毒眼感染。以前我们开发了 牛ac病毒角膜炎的定量兔模型，我们表明局部包含 治疗中的类固醇潜在危险。在接受泼尼松龙的所有组中，角膜炎 在停止治疗后的3-4天内反弹，这与 恢复病毒复制。小鼠模型更适合研究病理学 疫苗感染的免疫学因试剂的可用性。因此，目标的目标 此R21应用是开发一种新型的Vaccinia Virus Ocular的定量小鼠模型 感染。