Core Grant for Vision Research

视觉研究核心资助

• 批准号: 6945497
• 负责人: Curtis R Brandt
• 金额: $ 54.81万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6945497
• 关键词: biomedical facility; vision

DESCRIPTION (provided by applicant): This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules. These are Gene Delivery/Quantitative Molecular Biology,Pathology and Imaging, and Animal Models/ Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease. In addition, gene delivery to cells in culture is an essential research tool. The Gene Delivery Module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas will enhance the abilities of those researchers currently using these methods but more importantly, the core will make it easier for researchers currently not using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. These methodologies are also critical for studies using animal models. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assessing the effects of their agents, manipulations, and vectors/constructs on ocular function in rodents and primates. The services provided by these modules have been functioning for some time, but their ability to assist researchers has been limited by a lack of consistent support, and particularly by a lack of personnel. Funding of this proposal will enable us to improve our productivity and conduct work in a more timely manner, speeding the pace of progress in vision research on campus. It will allow us to expand the assistance available to eye researchers on campus and perhaps attract other investigators to eye research.

描述（由申请人提供）：威斯康星大学视觉研究人员的这项P30核心赠款提出了三个模块。这些是基因递送/定量分子生物学，病理学和成像以及动物模型/眼器官培养。有效地将基因传递到细胞的能力是治疗眼部疾病的一种强大的新治疗方法。此外，培养物中向细胞的基因递送是必不可少的研究工具。基因输送模块将构建向量，准备这些材料的高滴水库存供研究人员使用，并协助研究人员使用基因递送方法。定量方法在分子生物学中的使用正在增长。特别是，通过RT-PCR对基因表达的定量以及使用阵列技术的基因表达变化的分析。定量的分子生物学模块将为这些领域提供专门的技术专长，将增强目前使用这些方法的研究人员的能力，但更重要的是，目前未使用这些技术将其添加到其研究计划中的研究人员将变得更加容易。随着对翻译研究的重视，病理分析和复杂的成像方法变得越来越重要。这些方法对于使用动物模型的研究也至关重要。病理学和成像模块将提供组织切片和培养细胞的高级组织学，显微镜和图像分析以及形态计量学。新疗法的开发和提高我们对视觉系统功能的理解依赖于在体外进行的工作转移到动物模型中。动物模型和眼器官培养模块将帮助研究人员使用动物模型（灵长类动物和啮齿动物），实验性青光眼诱导，成像和眼生理学。这种专业知识将有助于核心用户在评估其代理，操纵和向量/构造对啮齿动物和灵长类动物中眼功能的影响方面的努力。这些模块提供的服务已经运行了一段时间，但是他们的协助研究人员的能力受到缺乏一致的支持，尤其是缺乏人员的限制。该提案的资金将使我们能够提高生产率并及时进行工作，从而加快校园视力研究进展的速度。这将使我们能够扩大校园中眼研究人员可用的援助，并可能吸引其他研究人员进行眼睛研究。