Spatial single cell analysis of the human Alzheimer's Disease brain

人类阿尔茨海默病大脑的空间单细胞分析

• 批准号: 10822988
• 负责人: Kirsten Frieda
• 金额: $ 122.93万
• 依托单位: SPATIAL GENOMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-21 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10822988
• 关键词: Address; Alzheimer' s Disease; Alzheimer' s disease brain; Animals; Antibodies; Area; Autopsy; Bar Codes; Biological Assay; Brain; Cell Communication; Cells; Collaborations; Collection; Complex; Computer software; Data; Data Set; Devices; Disease; Disease Progression; Environment; Epigenetic Process; Fluorescent in Situ Hybridization; Gene Expression; Gene Expression Profiling; Gene Order; Genes; Genomics; Geography; Gliosis; Goals; Human; Image; Immunofluorescence Immunologic; In Situ; In Situ Hybridization; Life; Maps; Measurement; Measures; Messenger RNA; Methods; Microglia; Molecular; Morphology; Mus; Neighborhoods; Neurofibrillary Tangles; Neurons; Noise; Pathology; Population; Procedures; Process; Protein Analysis; Proteins; Protocols documentation; RNA; RNA amplification; RNA analysis; Reaction; Reagent; Research Personnel; Resolution; Sampling; Scheme; Senile Plaques; Signal Transduction; Slice; Synapses; System; Technology; Tissues; Vascular Diseases; brain tissue; cell type; commercialization; experience; experimental study; genomic tools; human disease; instrument; multimodality; multiple omics; neuron loss; neuropathology; preservation; protein TDP-43; protein biomarkers; protein expression; protein profiling; single cell analysis; single molecule; transcriptome

SUMMARY The major goal of this project is to study human Alzheimer’s Disease (AD) brains using the Spatial Genomics GenePS instrument for automated large scale gene expression analysis at single cell resolution. At Spatial Genomics, we are commercializing the sequential fluorescence in situ hybridization (seqFISH) technology (Eng et al, 2019) that allows thousands of genes to be imaged directly in brain samples. We have experience working with human brains with our seqFISH technology and will hone the technology for use in human brains. Using the seqFISH method, we will identify changes in cell types, spatial organization of groups of cells, and signaling interactions between neighboring cells in the human AD brain.

概括 该项目的主要目标是利用 空间基因组学 GenePS 仪器，用于自动化大规模基因表达分析 在 Spatial Genomics，我们正在将顺序荧光商业化。 原位杂交 (seqFISH) 技术（Eng 等人，2019）允许数千个基因 直接在大脑样本中成像 我们拥有使用人类大脑进行研究的经验。 seqFISH 技术，并将磨练该技术在人类大脑中的应用。 方法，我们将识别细胞类型的变化、细胞群的空间组织，以及 人类 AD 大脑中相邻细胞之间的信号相互作用。