Spatial Genomics for in situ single cell expression analysis in the brain

用于大脑中原位单细胞表达分析的空间基因组学

• 批准号: 10254447
• 负责人: Kirsten Frieda
• 金额: $ 99.45万
• 依托单位: SPATIAL GENOMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10254447
• 关键词: Address; Adopted; Attention; BRAIN initiative; Brain; Cells; Computer software; Confocal Microscopy; Custom; Data; Data Set; Development; Familiarity; Fluorescent in Situ Hybridization; Future; Gene Expression; Gene Expression Profiling; Genes; Genomics; Human; Image; In Situ; Industry; Laboratories; Liquid substance; Marketing; Messenger RNA; Methods; Microscope; Modeling; Molecular; Mus; Nature; Neurodegenerative Disorders; Neurosciences; Pattern; Phase; Production; Publications; RNA; Research; Research Personnel; Running; Safety; Sampling; Site; Slice; Specific qualifier value; Standardization; Technology; Testing; Tissues; Training; base; brain cell; brain tissue; cell type; commercialization; experience; experimental study; gene panel; instrument; interest; novel; preservation; prototype; screening; single-cell RNA sequencing; touchscreen; transcriptome; transcriptomics; user-friendly

SUMMARY Spatial Genomics, Inc. is commercializing seqFISH technology that can visualize thousands of genes at the mRNA level in single cells with spatial information preserved. Recent advances now make it possible to achieve 10,000 gene multiplexing in single cells directly in tissues (Eng et al., Nature 2019). With this technology, novel cell types can be discovered in situ and spatial organizations can be directly identified and visualized in brain tissue sections.

概括 Spatial Genomics, Inc. 正在将 seqFISH 技术商业化，该技术可以可视化数千个 单细胞中 mRNA 水平的基因，保留了空间信息。现在的最新进展 使得直接在组织中的单细胞中实现 10,000 个基因多重化成为可能（Eng 等人， 自然 2019）。通过这项技术，可以在原位和空间发现新的细胞类型 可以在脑组织切片中直接识别和可视化组织。