Acquisition of an Oxford Nanopore sequencer for genomic analysis of a subterrestrial nematode

购买牛津纳米孔测序仪用于地下线虫的基因组分析

• 批准号: 10797580
• 负责人: John Russell Bracht
• 金额: $ 9.6万
• 依托单位: AMERICAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-07 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797580
• 关键词: Affect; American; Antibiotics; Apoptosis; Arginine; Astrocytes; Autoimmune; Autoimmune Diseases; Award; Caenorhabditis elegans; Cellular Stress; Development; Disease; Elements; Environment; Faculty; Family; Funding; Gene Expression; Genes; Genetic; Genetic Transcription; Genome; Genomics; Gold; Growth; Heat Stress Disorders; Heat-Shock Proteins 70; Heat-Shock Response; Human Pathology; Hypoxia; Investigation; Laboratories; Life; Link; Malignant Neoplasms; Maps; Measures; Methane; Molecular; Mutate; Nematoda; Nerve Degeneration; Neurodegenerative Disorders; Organism; Outsourcing; Parents; Pathway interactions; Phenotype; Play; Proteins; Publishing; Quantitative Reverse Transcriptase PCR; RNA; RNA Interference; Regulation; Research; Role; Sampling; Signal Transduction; Site; South Africa; South African; Starvation; Stress; Structure; Students; Technology; Testing; Therapeutic; Time; Toxin; Tumor stage; Tunicamycin; Underrepresented Students; Universities; Work; cell injury; cost; empowerment; environmental stressor; feeding; gene discovery; gene function; heat-shock factor 1; human disease; inhibitor; knock-down; nanopore; neoplastic; neurotrophic factor; novel; resilience; response; stress management; stress resilience; stressor; transcriptome sequencing; transcriptomics; undergraduate student

Abstract The supplemental funding will allow the purchase of an Oxford Nanopore sequencer to enable the Bracht laboratory to complete the aims of the parent award. The sequencing technology is critical given that 45 separate RNA samples must be sequenced and outsourcing the work is costly in both time and money. The Nanopore technology provides long, highly accurate reads enabling many exciting applications both for the parent award and beyond. The supplemental funding would provide a transformative technology, given there is currently no genomic sequencers available at American University; furthermore, there is no Nanopore technology available in the entire DC metro region containing at least five additional research universities beyond AU. This supplement would therefore fill a regional gap in technological availability, benefitting multiple universities and empowering the unique research conducted at American University: highly interdisciplinary, undergraduate-engaged and with a high engagement of under-represented students. Students and faculty alike would greatly benefit from the availability of a transformative Nanopore sequencer at AU. 1

抽象的 补充资金将允许购买牛津纳米孔测序仪，以实现 Bracht实验室完成了母奖的目标。测序技术是 鉴于必须对 45 个单独的 RNA 样本进行测序并且将这项工作外包，这一点至关重要 时间和金钱成本高昂。纳米孔技术提供长、高精度的读数 为家长奖及其他奖项提供许多令人兴奋的申请。补充的 鉴于目前还没有基因组学技术，资金将提供一项变革性技术 美国大学提供测序仪；此外，没有纳米孔技术 覆盖整个华盛顿都会区，其中至少还有五所研究型大学 超越非盟。因此，这一补充将填补技术可用性方面的区域差距， 使多所大学受益，并增强美国进行的独特研究的能力 大学：高度跨学科、本科生参与度高、学生参与度高 代表性不足的学生。学生和教师都将从中受益匪浅 AU 的变革性纳米孔测序仪。 1

项目成果

Evolution of a biological thermocouple by adaptation of cytochrome c oxidase in a subterrestrial metazoan.

通过适应地下后生动物中细胞色素 c 氧化酶的生物热电偶的进化。

• 发表时间: 2023-12-07
• 作者: Guerin, Megan N;Ellis, TreVaughn;Ware, Mark J;Manning, Alexandra;Coley, Ariana;Amini, Ali;Chung, George;Gunsalus, Kristin C;Bracht, John R
• 通讯作者: Bracht, John R