GENOTOXIC ASSAYS FOR EVALUATION OF DENTAL MATERIALS

用于评估牙科材料的基因毒性测定

基本信息

批准号：
3425311
负责人：
ANN J DUNIPACE
金额：
$ 2.21万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-08-01 至 1989-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3425311
关键词：
Salmonella biomaterial development /preparation biomaterial evaluation bone marrow exam chemical carcinogen composite resins dental materials hamsters laboratory mouse microsomes mutagen testing mutagens physical chemical interaction

项目摘要

Dental materials are placed in direct contact with living tissues. Their biological effects must be determined and their compatibility with oral tissues and general health must be assesed in order to insure their safe use in the treatment of patients. Earlier protocols for determining the safety of dental materials have focused on their general toxicity and local, tissue irriting properties with no extensive systematic assays proposed for mutagenic evaluation of dental materials. Because mutations can be related to a variety of serious diseases, assessment of the mutagenic potential of dental materials is essential. At present there is limited information about the mutagenic effects of dental materials, and the conclusions from most previous studies have been based only on results from the Ames Test. Mutagenicity assays currently available each have their strengths and weaknessess. Therefore, the mutagenic potential of a material or its componets cannot be reliably determined by a single method but must be analyzed by a battery of tests. The hypothesis which will be tested in this proposed investigation is that the in vitro Ames Test may not adequately evaluate the genotoxic potential of dental materials and that additional, in vivo genotoxic assays are needed in order to reliably evaluate mutagenic potential of these materials. The study is designed to investigate the application of a composite of genotoxic test procedures for evaluating the application of a composite of genotoxic test procedures for evaluating the mutagenicity of dental materials. The following four genotoxic assays will be used in this study: 1) in vitro Ames Salmonella microsome assays; 2) the in vivo mouse bone marrow micronucleus test; 3) the in vivo mouse sperm morphology test; and 4) in vivo sister chromatid exchange (SCE) studies in chinese hamster bone marrow cells. These test include a variety of systems from the simple bacterium to mammalian cells and should allow more compresensive evaluation and mutagenic potential od dental materials. This investigation will determine the applicability and adapatability of these established genotoxic tests for subsequent evaluations of dental materials.

牙科材料与活组织直接接触。必须确定它们的生物学效果并兼容必须评估口服组织和一般健康确保他们在治疗患者方面的安全使用。确定牙科材料安全的早期协议专注于他们的一般毒性和局部组织刺激没有提出广泛系统性测定的特性牙科材料的诱变评估。因为突变可以是与各种严重疾病有关，评估牙科材料的诱变潜力至关重要。现在关于牙齿的诱变作用的信息有限材料，大多数先前研究的结论是仅基于AMES测试的结果。诱变性测定目前可用的每个人都有自己的优势和缺点。因此，材料或其组合的诱变潜力不能通过一种方法可靠地确定，但必须是通过一系列测试分析。该假设将在此拟议的研究中进行检验是体外AMES测试可能无法充分评估牙科材料的遗传毒性潜力，并在为了可靠评估，需要体内遗传毒性测定这些材料的诱变潜力。该研究旨在研究一组遗传毒性测试的应用评估复合材料的应用程序用于评估牙齿诱变性的遗传毒性测试程序材料。以下四个遗传毒性测定将用于这项研究：1）体外AMES沙门氏菌微粒体测定； 2）体内小鼠骨髓微核测试； 3）体内鼠标精子形态测试； 4）体内姐妹染色质酸交换（SCE）中国仓鼠骨髓细胞的研究。这些测试包括从简单细菌到哺乳动物的各种系统细胞，应允许更多的评估和诱变潜在的OD牙科材料。这项调查将确定这些已建立的遗传毒性的适用性和适应性对随后评估牙科材料的测试。