WNPRC ANIMAL SERVICES DIVISION

WNPRC 动物服务部

基本信息

批准号：
7958730
负责人：
Saverio Vincent Capuano
金额：
$ 68.81万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7958730
关键词：
Acquired Immunodeficiency Syndrome American Animal Barrier Facility Animal Experimentation Animal Husbandry Animal Technicians Animal Welfare Animals Autopsy Award Behavior Behavior Therapy Behavior assessment Behavioral Biocompatible Materials Biopsy Blood Blood specimen Breeding Callithrix Callithrix jacchus jacchus Caring Certification Chemicals Child Clinical Clinical Pathology Collaborations Collection Communication Communities Computer Retrieval of Information on Scientific Projects Database Consultations Continuing Education Control Animal Data Databases Decision Making Development Disease Documentation Drug or chemical Tissue Distribution Electronics Emergency Situation Employee Ensure Environment Equipment Equipment and supply inventories Food Fostering Funding Genetic Materials Goals Grant Hand Health Hematologist Housing Human Human Resources IACUC Individual Industry Information Services Institution Instruction International Internet Journals Laboratories Laboratory Animal Science Libraries Location Macaca Macaca mulatta Maintenance Manuscripts Medical Minor Mission Monitor Occupational Occupational Health Operative Surgical Procedures Organ Output Pathologist Pathology Peer Review Police officer Policies Positive Reinforcements Preventive Medicine Primates Principal Investigator Procedures Process Progress Reports Prospective Studies Protocols documentation Publications Quality Control Quarantine Records Regulation Reporting Research Research Infrastructure Research Personnel Research Project Grants Research Support Research Training Resource Allocation Resources Retrieval Safety Sampling Schools Self-Injurious Behavior Sensory Services Site Visit Slide Societies Source Specimen Students Techniques Time Tissue Banking Tissue Banks Tissue Sample Tissues Toy Training United States National Institutes of Health Universities Vendor Veterinarians Visit Wisconsin Work aged animal care animal tissue base clinical care college design environmental enrichment for laboratory animals follow-up girls improved journal article lectures meetings member nonhuman primate occupational health/safety outreach program planetary Atmosphere programs rapid diagnosis research study response sample collection skills social species typical behavior success symposium virtual web site

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I. Overview and Objectives: The Animal Services Division is composed of 5 units (Veterinary Services, Colony Management, Pathology Services, Compliance and Training, and Behavioral Management) that are dedicated to maintaining the health of the nonhuman primate (NHP) colonies of the WNPRC; supporting the scientific mission of the Center, ensuring regulatory compliance, and training personnel to work safely with NHPs and their tissues. Each of the five units performs their own individual duties but also act in synergy to fulfill the specific aims of the division. Furthermore, the individual units interact with the other divisions to fulfill the goals of the entire Center. A. Veterinary Services Unit The Veterinary Services Unit of the WNPRC employs six veterinarians and seven veterinary technicians. The principal objective of the unit is to provide consistent and excellent care to the NHP colonies housed at the WNPRC. To achieve this objective, the unit has created and implemented a collection of policies that guide the provision of veterinary care at the WNPRC. These policies, which encompass pertinent topics such as quarantine, preventative medicine, and clinical/surgical care, are based on the most contemporary techniques available to NHP veterinarians. The second major objective of the unit is to provide support for the investigators performing research at the WNPRC. The primary way in which the unit supports the investigators is by ensuring a consistent supply of healthy NHPs is available for utilization in experimental studies. The veterinary staff also assists investigators by meeting with them prior to an experiment to discuss and plan for each proposed grant and study, by helping compose IACUC protocols, by performing procedures beyond the skill level of the investigators, and by providing clinical care for the NHPs assigned to research projects. The final objective of the unit is to provide training for personnel working with NHPs at the WNPRC and at other institutions. Through didactic and applied instruction, the unit provides training to veterinary and veterinary technical students, visiting veterinarians and veterinary technicians, WNPRC and visiting investigators, and scientific support staff. The Veterinary Services Unit works closely with the other units of the Animal Services Division as well other divisions of the WNPRC. This collaboration ensures the most complete and effective care of our animals and benefits the Veterinary Services unit as well the other individual units and divisions of the WNPRC. B. Colony Management Unit The Colony Management Unit employs a colony manager, 4 supervisors, 24 full-time and 4 part-time animal research technicians, 2 macaque and 1 marmoset breeding coordinator, and 2 colony records assistants. The Colony Management Unit is one of the most crucial components of the WNPRC infrastructure as it supports the entire animal husbandry program of the Center. The daily tasks performed by the Colony Management Unit include: + Provision of food to the nonhuman primate colonies + Sanitization of the nonhuman primates caging, rooms, equipment, and the remainder of the animal barrier facilities + Maintenance of all records related to HVAC and sanitization processes + Monitoring and minor maintenance of automatic cage washers and autoclaves In addition to their husbandry duties, the personnel of the Colony Management Unit perform pivotal tasks for the Veterinary Services, Behavioral Management, Compliances & Training, Pathology Services, Purchasing, and the Centralized Protocol Implementation Units of the Center. These tasks include, but are not limited to the following: + Documentation and communication of daily health reports on the nonhuman primate colonies + Maintenance of colony records + Collection of behavioral and scientific data + Administration of medical and experimental treatments + Provision of environmental enrichment objects and documentation of this provision + Collection of blood and other tissue samples for experimental and clinical purposes + Completion of inventory tasks for the Purchasing Unit for all sanitization supplies, chemicals, and personal protective equipment + In collaboration with the Training Coordinator: o Provision of training to all personnel and any outside colleagues who may come in contact with nonhuman primates at the WNPRC o Provision of continuing education opportunities in the form of mini-lectures and involvement in the American Association for Laboratory Animals Science lectures and certification programs Finally, in cooperation with the Senior Management Team, the Colony Manager maintains the NHP breeding colonies of the Center and ensures that a sufficient supply of healthy, genetically characterized diverse animals are available to core, collaborative, and outside investigators. C. Colony Records Subunit One full-time and one part-time assistant staff this subunit of Colony Management. The subunit's staff is responsible for the daily entry of all data related to the nonhuman primate colony including clinical procedures, animal location transfers, treatments, research procedures, surgical procedures, sample collections, and health observations. Colony Records staff also assure the quality control of data entries, train new staff on how to use the Animal Records Database, and perform database queries. The subunit is appropriately situated in the Colony Management unit to foster frequent communication among personnel performing the work being documented. The unit works closely with the Information Services Division of the WNPRC to continuously improve the Internet-based version of the Colony Records Database and to facilitate and simplify the data entry and retrieval process. D. Behavioral Management Unit The Behavioral management Unit employs a coordinator, two full-time assistants and on student. The Behavioral Management staff utilizes a combination of environmental enrichment, behavior modification, and positive reinforcement, in attempt to promote a diverse array of species typical behaviors in the captive NHPs, increase each animal's control and utilization of their environment, and decrease the occurrence of stereotypical and self-injurious behavior. The Environmental Enrichment Plan remains unchanged from last year. This plan consists of the following components: + Social companionship + Foraging + Food enrichment + Human to non-human interaction + Structural enrichment + Manipulanda + Additional sensory stimulation Each component of the enrichment plan has been designed to work in unison with the other components to create a comfortable and psychologically challenging environment for the NHPs. E. Compliance and Training Unit The Compliance and Training Unit employs a Compliance Coordinator, a Training Coordinator, a part-time Training Assistant, and a part-time Laboratory Safety Coordinator. Compliance and Training is a multi-dimensional unit with a variety of responsibilities. The primary objectives of the unit are to train all personnel at the WNPRC who may come in contact with NHPs or their tissue; to ensure that the WNPRC facilities and personnel remain compliant with all animal welfare regulations; to assist investigators in the development of IACUC protocols; and to guide the Occupational Health and Safety Program of the Center. Since its inception in 2005, the unit has worked in collaboration with the various divisions and investigators of the WNPRC to standardize training and promote a center-wide atmosphere of regulatory compliance and safety. F. Pathology Services Unit The Pathology Services Unit employs two part-time Veterinary Pathologists, a full-time Hematologist/Laboratory Safety Coordinator, and two full-time research assistants. The unit is currently engaged in a nationwide search for another veterinary pathologist. The Pathology Services unit has been an integral part of the WNPRC since it's inception. Pathology services has two main functions 1) to support nonhuman primate colony health and the clinical veterinary staff by providing rapid diagnoses of disease and 2) to support all research projects at the WNPRC that involve terminal procedures, surgical biopsies and/or clinical pathology. Complete gross necropsies are performed on all animals, whether they are euthanized for clinical reasons, die spontaneously, or are euthanized for experimental purposes. Tissues from these animals receive complete histopathological examinations, except when the examination of a specific tissue is incompatible with experimental protocols. The tissue distribution program provides primate tissue to investigators affiliated with WNPRC, the University of Wisconsin, other academic institutions, and private industry. A comprehensive tissue library of both rhesus macaque and common marmoset tissues is maintained for both retrospective and prospective studies. G. Allocation of Resource Access The Animal Services Division works closely with the Centralized Protocol Implementation Unit and the WNPRC Executive Committee to review all requests for access to the Center's nonhuman primate colonies. Equitable decisions are made by these three entities to ensure that all PIs have access to the nonhuman primates they require for their IACUC-approved experimental protocols. Pathology Services notifies investigators of the resource via the Primate Center website, scientific meetings, and through the nonhuman primate biological materials distribution program. To date, no requests have been denied. All core WNPRC labs use Pathology Services at some point in their projects, and numerous campus affiliates avail of this service. In addition, since May 1, 2008, 12 investigators from non-host institutions received a total of 140 samples, representing tissues, organs and genetic material from Macaca mulatta, Macaca fasicularis, and Callithrix jacchus. Please see the Research Services section of this progress report for a table of species, specimen types and numbers. H. Dissemination The Associate Director of Animal Services strives to ensure that important information discovered or developed by the division is disseminated to the greater scientific community through publication in peer-reviewed journals and at national meetings. We request that projects utilizing any divisional services acknowledge the service in manuscripts and presentations. Animal Services personnel also lecture regularly on nonhuman primate topics to WNPRC staff, and to veterinary residents and students on campus. One unit member presented a short outreach program to a local Girl Scout troop that involved the creation of enrichment toys for animals and a follow-up meeting so that the children could see the results of their work. Another unit member, one of the veterinarians, gave several talks to visiting school groups at the Primate Center in FY2008-2009. I. Progress and Highlights Veterinary Services - In 2008, the Veterinary Services Unit fulfilled their three main objectives (animal care, research support, and training) admirably. The unit was pivotal in the success of site visits performed by the USDA and AAALAC. The unit also provided training for 9 vet tech students, 14 vet students, 4 WNPRC investigators, and 6 veterinary personnel from facilities outside of the WNPRC. The surgical subunit of Veterinary Services provided support for 197 surgical procedures. Members of the Veterinary Services Unit also presented lectures at two national meeting in 2008. Colony Services In addition to daily husbandry responsibilities the personnel of the Colony Services Unit provided a pivotal service for the Veterinary Unit and the investigators of the WNPRC. In 2008, the unit cleaned greater than 400,000 cages, administered 19,000 treatments, and collected 5,840 blood samples. Behavioral Management - The Behavioral Management Unit continues to develop their overall program from simply providing environmental enrichment and pairing animals to generating true academic output that is relevant to the field of nonhuman primate behavior. In 2008, the unit has expanded its environmental enrichment program for marmosets, standardized the behavioral assessment program, incorporated more behavioral data into the colony records database, provided approximately 98,000 enrichment opportunities for the colony, and has presented data at the annual meet of the American Society of Primatologists. Compliance and Training - With the addition of a part-time Laboratory Safety Coordinator in 2008, the Compliance and Training Unit continued to focus on the improvement of laboratory safety training, general safety training, appropriate responses to emergency situations, and hands on training for research staff with husbandry and animal handling duties. Other accomplishments included providing refresher training in basic biosafety to all laboratory staff and the provision of Laboratory Training Notebooks to all principal investigators. In 2008, nearly all standard operating procedures were carefully re-evaluated and revised to be more specific with their content, and an effective electronic verification form was developed to ensure compliance with review of revised SOPs. The unit provided training regarding occupational health information for 390 people working with NHPs. The individuals trained were from a variety of occupational backgrounds including media, custodial staff, maintenance personnel, vendors, police officers, national/international visitors, students and new employees. Additionally, the unit performed 45 protocol pre-reviews for investigators in 2008. Pathology Services - The unit has continued to function smoothly during 2008. Unit members have participated in and rotationally hosted the monthly virtual slide conference for Primate Pathology. One pathologist presented a talk at the annual American College of Veterinary Pathologists (ACVP) meeting. In 2008, the unit was awarded responsibility for the collection, inventory, and dissemination of samples for the NIH Aged Nonhuman Primate Tissue Bank. In 2008 Pathology Unit members provided training in specialized NHP necropsy procedures to campus pathologists, investigators, multiple consultations on NHP cases. Unit members also provided preparatory training for the American College of Veterinary Pathologists certifying examination to four pathology residents on campus. Publications note: Animal Services support is involved in practically every journal article that depends in part or in full on WNPRC resources. Note: AIDS related

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。 I.概述和目标：动物服务部由5个单位（兽医服务，殖民地管理，病理服务，合规性和培训以及行为管理）组成，这些单位致力于维持WNPRC的非人类灵长类动物（NHP）殖民地的健康；支持该中心的科学任务，确保监管合规性和培训人员与NHP及其组织安全合作。这五个单元中的每一个都执行自己的个人职责，但也以协同作用来实现该部门的具体目标。此外，各个单位与其他部门进行互动以实现整个中心的目标。 A.兽医服务部门 WNPRC的兽医服务部门雇用了六名兽医和七位兽医技术人员。该部门的主要目标是为WNPRC所容纳的NHP殖民地提供一致和出色的护理。为了实现这一目标，该部门制定并实施了一系列政策，以指导WNPRC提供兽医护理。这些政策涵盖了相关主题，例如隔离，预防医学和临床/手术护理，基于NHP兽医可用的最现代技术。该部门的第二个主要目标是为在WNPRC进行研究的研究人员提供支持。该单元支持研究人员的主要方法是确保在实验研究中可用于利用健康的NHP的一致供应。兽医人员还通过在实验之前与研究人员会面，以讨论和计划每个提议的赠款和研究，通过帮助撰写IACUC方案，通过执行超出研究人员技能水平的程序，并为分配给研究项目的NHP提供临床护理，以帮助组成IACUC方案。该部门的最终目的是为WNPRC和其他机构的NHPS工作人员提供培训。通过教学和应用指导，该部门为兽医和兽医技术学生提供培训，访问兽医和兽医技术人员，WNPRC和访客研究人员以及科学支持人员。兽医部门与动物服务部的其他部门以及WNPRC的其他部门紧密合作。这项合作确保了对我们的动物的最完整和最有效的护理，并使WNPRC的其他单独单位和部门受益。 B.殖民地管理部门殖民地管理部门雇用了一名殖民地经理，4位主管，24名全日制和4名兼职动物研究技术人员，2名猕猴和1名Marmoset育种协调员，以及2名Colony Records Records助手。殖民地管理部门是WNPRC基础设施中最关键的组成部分之一，因为它支持该中心的整个畜牧计划。殖民地管理部门执行的日常任务包括： +向非人类灵长类动物殖民提供食物 +非人类灵长类动物笼子，房间，设备和其余动物屏障设施的消毒 +维护与HVAC和消毒过程有关的所有记录 +自动笼垫圈和高压灭菌器的监视和次要维护除了饲养职责外，殖民地管理部门的人员还针对兽医服务，行为管理，合规和培训，病理学服务，采购以及中心的集中协议实施单位执行关键任务。这些任务包括但不限于以下内容： +关于非人类灵长类动物殖民的日常健康报告的文档和通讯 +维护殖民记录 +收集行为和科学数据 +医疗和实验治疗的管理 +提供环境丰富对象和此规定的文档 +用于实验和临床目的的血液和其他组织样品收集 +完成所有消毒用品，化学药品和个人保护设备的采购单元的库存任务 +与培训协调员合作： o向所有人员和任何可能与WNPRC的非人类灵长类动物接触的人提供培训 o以迷你讲座的形式提供继续教育的机会，并参与美国实验动物科学协会讲座和认证计划最后，与高级管理团队合作，殖民地经理维护了中心的NHP繁殖殖民地，并确保核心，协作和外部研究人员可以提供足够的健康，遗传特征的多种动物。 C.殖民记录亚基一名全职和一名兼职助理员工这个殖民地管理的亚基。亚基的工作人员负责每天进入与非人类灵长类动物群体有关的所有数据，包括临床程序，动物位置转移，治疗，研究程序，外科手术程序，样本收集和健康观察结果。 Colony Records员工还确保数据条目的质量控制，培训新员工如何使用动物记录数据库并执行数据库查询。该亚基适当地位于殖民地管理部门，以促进执行所记录工作的人员之间的频繁沟通。该单元与WNPRC的信息服务部紧密合作，以不断改进Colony Records数据库的基于Internet的版本，并促进和简化数据输入和检索过程。 D.行为管理单位行为管理部门雇用了协调员，两个全职助手和学生。行为管理人员利用环境富集，行为修改和积极的强化结合，试图促进圈养的NHP中各种各样的物种典型行为，增加每只动物对环境的控制和利用，并减少刻板印象的发生。与去年相比，环境丰富计划保持不变。该计划由以下组成部分组成： +社会陪伴 +觅食 +食物富集 +人与非人类互动 +结构丰富 + Manipulanda +其他感觉刺激丰富计划的每个组成部分均设计为与其他组件一致工作，以创造一个舒适且心理上具有挑战性的NHP环境。 E.合规性和培训单位合规性和培训部门采用合规协调员，培训协调员，兼职培训助理和兼职实验室安全协调员。合规性和培训是一个多维单位，具有多种责任。该部门的主要目标是培训可能与NHP或组织接触的WNPRC的所有人员；确保WNPRC设施和人员遵守所有动物福利法规；协助研究人员制定IACUC方案；并指导中心的职业健康与安全计划。自2005年成立以来，该部门一直与WNPRC的各个部门和调查人员合作，以标准化培训并促进中心范围的监管合规性和安全性。 F.病理服务部门病理服务部门雇用了两名兼职兽医病理学家，全职血液学家/实验室安全协调员和两名全日制研究助理。该部门目前正在全国范围内寻找另一位兽医病理学家。自成立以来，病理服务部门一直是WNPRC不可或缺的一部分。病理服务具有两个主要功能1）通过提供疾病的快速诊断和2）支持WNPRC的所有研究项目，以支持非人类灵长类动物殖民健康和临床兽医，并支持终端程序，手术活检和/或临床病理学。无论是出于临床原因而对所有动物进行安乐死，都会自发死亡，还是为实验目的进行安乐死。这些动物的组织接受了完整的组织病理学检查，除非对特定组织的检查与实验方案不相容。组织分配计划为与WNPRC，威斯康星大学，其他学术机构和私营企业的研究人员提供了灵长类组织。用于回顾性研究和前瞻性研究，都保持了恒河猕猴和常见的果果组织组织的全面组织文库。 G.资源访问的分配动物服务部与集中协议实施部门和WNPRC执行委员会密切合作，以审查所有访问该中心非人类灵长类动物殖民地的要求。这三个实体是由公平决定做出的，以确保所有PI都可以访问其IACUC批准的实验方案所需的非人类灵长类动物。病理服务通过灵长类动物中心网站，科学会议以及非人类灵长类动物生物材料分销计划通知研究人员。迄今为止，尚未拒绝要求。所有核心WNPRC实验室在其项目的某个时候都使用病理服务，并且众多校园分支机构利用此服务。此外，自2008年5月1日以来，来自非宿主机构的12名研究人员总共收到了140个样本，代表来自Macaca Mulatta，Macaca Fasicularis和Callithrix Jacchus的组织，器官和遗传物质。请参阅此进度报告的研究服务部分，以了解物种，标本类型和数字的表。 H.传播动物服务副主任致力于确保该部门发现或开发的重要信息通过在同行评审期刊和全国会议上的出版而传播给更大的科学界。我们要求使用任何部门服务的项目确认手稿和演示中的服务。动物服务人员还定期向WNPRC工作人员以及校园内的兽医居民和学生讲授非人类灵长类动物主题。一名单位成员向当地的女童子军部队提出了一个简短的外展计划，该计划涉及为动物创建丰富玩具和一次后续会议，以便孩子们可以看到他们的工作结果。另一位单位成员是其中一位兽医，在2008-2009财年的灵长类动物中心与访问学校团体进行了几次会谈。 I.进步和亮点兽医服务 - 2008年，兽医服务部门实现了他们的三个主要目标（动物护理，研究支持和培训）。该单元在USDA和AAALAC进行的现场访问成功中至关重要。该部门还为9名兽医科技学生，14名兽医学生，4名WNPRC调查人员和6名兽医人员提供了培训，该学生来自WNPRC以外的设施。兽医服务的手术亚基为197个手术程序提供了支持。兽医服务部门的成员还在2008年的两次全国会议上发表了讲座。殖民地服务除了每日饲养职责外，殖民地服务部门的人员为兽医部门和WNPRC的调查人员提供了关键服务。 2008年，该单元清洁了超过400,000个笼子，进行了19,000种处理，并收集了5,840个血液样本。行为管理 - 行为管理部门继续开发其整体计划，从简单地提供环境丰富和配对动物到产生与非人类灵长类动物行为领域相关的真实学术成果。 2008年，该部门扩大了其针对马尔莫斯人的环境丰富计划，对行为评估计划进行了标准化，将更多的行为数据纳入了殖民地记录数据库中，为殖民地提供了大约98,000个富集机会，并在美国原始学家的年度会议上介绍了数据。合规性和培训 - 在2008年增加了兼职实验室安全协调员，合规性和培训部门继续专注于改善实验室安全培训，一般安全培训，对紧急情况的适当回应以及动手为养老机和动物处理职责的研究人员进行培训。其他成就包括向所有实验室人员提供基本生物安全的进修培训，以及向所有主要研究人员提供实验室培训笔记本。在2008年，对几乎所有标准的操作程序进行了仔细的重新评估和修订，以使其内容更具体，并制定了有效的电子验证表，以确保遵守对修订后的SOP的审查。该部门为390名与NHPS合作的人提供了有关职业健康信息的培训。受过培训的个人来自各种职业背景，包括媒体，托管人员，维护人员，供应商，警察，国家/国际游客，学生和新员工。此外，该单元在2008年对研究人员进行了45个协议预审。病理服务 - 该部门在2008年期间继续顺利运行。单位成员参加了灵长类病理学的每月虚拟幻灯片会议，并旋转了。一位病理学家在一年一度的美国兽医病理学家学院（ACVP）会议上发表了演讲。 2008年，该部门因对NIH年龄非人类灵长类动物组织的收集，清单和传播样品的收集，库存和传播授予了责任。 2008年，病理单位成员为校园病理学家，研究人员，关于NHP病例的多次咨询提供了专门的NHP尸检程序的培训。单位成员还为美国兽医病理学家学院提供了预科培训，并向校园的四名病理居民进行检查。出版物注：动物服务的支持实际上涉及每条期刊文章，部分依赖于WNPRC资源。注意：与艾滋病有关