Oklahoma Nathan Shock Center of Excellence in Basic Biology of Aging

俄克拉荷马州内森休克衰老基础生物学卓越中心

基本信息

批准号：
10649612
负责人：
Veronica Galvan
金额：
$ 106.06万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-15 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10649612
关键词：
Address Aging Award Bioinformatics Biological Assay Biology Biology of Aging Cell Culture Techniques Cells Centers of Research Excellence Cities Communities Country Couples DNA Data Data Analyses Data Set Development Disease Education Environment Epigenetic Process Equipment Faculty Foundations Free Radicals Funding Future Generations Genes Genome Geroscience Goals Grant Health Sciences Human Image In Situ Individual Institution Intervention Label Mass Spectrum Analysis Measurement Measures Medical Research Medical center Mentors Methods Methylation Mitochondria Modeling Molecular Target Mus Mutation Oklahoma Organism Oxidation-Reduction Pathologic Pathway interactions Population Production Protein Analysis Protein Biosynthesis Proteins Proteomics Rattus Reactive Oxygen Species Research Research Personnel Resources Risk Factors Rodent Running Sampling Services Shock Site Structure Techniques Technology Tissues Training Travel United States Universities Variant age effect age related animal tissue bioinformatics tool career career development design genome sciences genome-wide graduate student heteroplasmy high throughput analysis in vivo innovation instrumentation large datasets mitochondrial genome next generation sequencing novel novel strategies outreach oxidative damage programs protein degradation recruit research and development symposium transcriptomics

项目摘要

The Oklahoma Shock Nathan Shock Center is designed to deliver unique, innovative services that are not currently available at most institutions or at other Shock Centers. The focus of the Center is on the development of careers of young investigators but our services are accessible by the entire research community studying aging and Geroscience. The Oklahoma Shock Center is unique in that it focuses on the field of Geroscience and has institutional support from the three major research institutions in Oklahoma City: the University of Oklahoma Health Science Center, the Oklahoma Medical Research Foundation, and the Oklahoma City VA Medical Center. Over the past four years, more than 75% of the Pilot Grants awarded by the Research Development Core and almost 40% of all services provided researchers by the Research Cores have been provided to investigators at institutions other than the host institutions. The 2020 Oklahoma Nathan Shock Center has six Cores. The Program Enhancement Core oversees all the activities of the Shock Center and organizes the annual Oklahoma GeroScience Symposium, which provides travel awards to graduate students and fellows from other institutions to attend the Symposium. The Geropathology Research Resource in the Core provides investigators with pathological assessments of mice using the Geropathology Grading Platform. The Research Development Core is an innovative departure from the traditional structure of similar Cores in Nathan Shock Centers and is designed to provide unprecedented service to the scientific community throughout the nation. The Multiplexing Protein Analysis Core provides investigators novel mass spectrometry methods for measuring the abundance of proteins from any tissue/cell from any organism that has its genome sequenced. The Core can quantify panels of ~50 proteins in a single run. Using D2O labeling, the Core can also measure the synthesis and turnover of these proteins. In combination, these analyses facilitate interrogation of proteomic changes in pathways of importance to aging and Geroscience. The Genomic Sciences Core has developed assays to measure both the methylation and hydroxymethylation of DNA at specific sites in the genome, which will be available to investigators studying epigenetics of aging. The Core also offers researchers the comprehensive analysis of mitochondrial genome heteroplasmy (variants/mutations and deletions) and copy number as well as single-cell transcriptomics assays. The Geroscience Redox Biology Core provides investigators with measures of oxidative damage, redox couples, and mitochondrial function, which require specialized instrumentation and expertise. The Core also provides investigators with the latest technology to measure reactive oxygen species in vivo and in situ in tissues of rodents. The GeroInformatics Core provides investigators assistance in the analysis of large datasets, statistical support, analysis of high-throughput datasets including single-cell transcriptomic data and has developed unique bioinformatics tools and methods to assist investigators to discover new connections between their data and age-related phenomenon.

俄克拉荷马震动队内森震动中心旨在提供独特、创新的服务，这些服务不是目前大多数机构或其他休克中心都提供。中心的重点是发展年轻研究人员的职业生涯，但整个研究社区都可以使用我们的服务老龄化和老年科学。俄克拉荷马州休克中心的独特之处在于它专注于老年科学领域拥有俄克拉荷马城三大研究机构的机构支持：俄克拉荷马大学健康科学中心、俄克拉荷马医学研究基金会和俄克拉荷马城退伍军人医疗中心。过去四年，超过 75% 的试点补助金由研究发展核心和研究核心为研究人员提供的所有服务中，近 40% 已提供给以下机构的研究人员：主办机构以外的机构。 2020 年俄克拉荷马州内森休克中心有六个核心。这计划增强核心监督休克中心的所有活动并组织年度活动俄克拉荷马州老年科学研讨会，为研究生和其他国家的研究员提供旅行奖励机构参加研讨会。核心的老年病理学研究资源为研究人员提供使用老年病理学分级平台对小鼠进行病理评估。研究进展 Core 是对 Nathan 减震中心类似 Core 的传统结构的创新背离，旨在为全国科学界提供前所未有的服务。多路复用蛋白质分析核心为研究人员提供了测量丰度的新颖质谱方法来自基因组已测序的任何生物体的任何组织/细胞的蛋白质。 Core可以量化面板单次运行约 50 种蛋白质。使用 D2O 标记，Core 还可以测量以下物质的合成和周转：这些蛋白质。结合起来，这些分析有助于探究途径中的蛋白质组变化对老龄化和老年科学具有重要意义。基因组科学核心已开发出检测方法来测量 DNA在基因组特定位点的甲基化和羟甲基化，这将是可供研究衰老表观遗传学的研究人员使用。该核心还为研究人员提供了线粒体基因组异质性（变异/突变和缺失）和拷贝的综合分析数量以及单细胞转录组学测定。 Geroscience 氧化还原生物学核心提供研究人员测量氧化损伤、氧化还原对和线粒体功能，需要专门的仪器和专业知识。该核心还为调查人员提供了最新的测量啮齿动物体内和原位组织中活性氧的技术。这 GeroInformatics Core 为研究人员提供分析大型数据集、统计数据的帮助支持、分析高通量数据集，包括单细胞转录组数据，并具有开发独特的生物信息学工具和方法来协助研究人员发现新的联系他们的数据和与年龄相关的现象之间。

项目成果

期刊论文数量（110）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ALE: automated label extraction from GEO metadata.

ALE：从 GEO 元数据中自动提取标签。

DOI：
发表时间：
2017
期刊：
影响因子：
3
作者：
Giles, Cory B;Brown, Chase A;Ripperger, Michael;Dennis, Zane;Roopnarinesingh, Xiavan;Porter, Hunter;Perz, Aleksandra;Wren, Jonathan D
通讯作者：
Wren, Jonathan D

Algorithmically outsourcing the detection of statistical errors and other problems.

从算法上外包统计错误和其他问题的检测。

DOI：
发表时间：
2018
期刊：
影响因子：
0
作者：
Wren; Jonathan D
通讯作者：
Jonathan D

Caloric restriction mitigates age-associated hippocampal differential CG and non-CG methylation.

热量限制可减轻与年龄相关的海马差异 CG 和非 CG 甲基化。

DOI：
发表时间：
2018
期刊：
影响因子：
4.2
作者：
Hadad, Niran;Unnikrishnan, Archana;Jackson, Jordan A;Masser, Dustin R;Otalora, Laura;Stanford, David R;Richardson, Arlan;Freeman, Willard M
通讯作者：
Freeman, Willard M

Chemically induced carcinogenesis in rodent models of aging: assessing organismal resilience to genotoxic stressors in geroscience research.

啮齿动物衰老模型中化学诱发的致癌作用：评估老年科学研究中对基因毒性应激源的机体恢复能力。

DOI：
发表时间：
2019
期刊：
影响因子：
5.6
作者：
Csiszar, Anna;Balasubramanian, Priya;Tarantini, Stefano;Yabluchanskiy, Andriy;Zhang, Xin A;Springo, Zsolt;Benbrook, Doris;Sonntag, William E;Ungvari, Zoltan
通讯作者：
Ungvari, Zoltan

Heart and neural crest derivative 2-induced preservation of sympathetic neurons attenuates sarcopenia with aging.

心脏和神经嵴衍生物 2 诱导的交感神经元保存可减轻衰老过程中的肌肉减少症。

DOI：
发表时间：
2021
期刊：
影响因子：
0
作者：
Rodrigues, Anna Carolina Zaia;Wang, Zhong;Messi, María Laura;Bonilla, Henry Jacob;Liu, Liang;Freeman, Willard M;Delbono, Osvaldo
通讯作者：
Delbono, Osvaldo