Husbandry and Management

畜牧与管理

• 批准号: 10647881
• 负责人: Melween I. Martinez
• 金额: $ 256.83万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10647881
• 关键词: Accreditation; Acquired Immunodeficiency Syndrome; Age; Alleles; Animal Testing; Animals; Biomedical Research; Breeding; Budgets; Caribbean region; Caring; Characteristics; Communities; Ensure; Female; Funding; Funding Mechanisms; Genetic; Goals; Grant; Health; Heterozygote; IACUC; Inbreeding; Income; Institution; Laboratory Animals; Macaca mulatta; Maintenance; Medical; Monitor; Monkeys; Population Genetics; Primates; Puerto Rico; Recovery; Regulation; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Safety; Sales; Science; Services; Site Visit; Solid; Stratification; Testing; Time; United States Department of Agriculture; United States National Institutes of Health; Universities; Viral; Virus; Visit; Work; animal care; cohort; cost; genetic analysis; germ free condition; improved; male; offspring; operation; programs

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. We plan to attain the following specific aims during the execution of the proposed plan in this Core: Aim 1: To maintain the closed CPRC- SPF indian-origin rhesus macaques colony, at a steady state level, that can provide high quality animals to support numerous NIH funded research projects, and to continue working toward self- sufficiency. The SPF colony will be maintained in compliance with the regulatory bodies. Aim 2. To use the projected recovery cost income, as part of an integral financial plan, to support the CPRC-SPF colony. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目概要/摘要： 此应用程序的主要目标是维持加勒比海地区的无特定病原体 (SPF) 计划 灵长类动物研究中心 (CPRC) 通过运营、管理、兽医护理、病毒学支持 MHC 分型以及我们综合遗传管理计划的改进。一个独特的特点是 CPRC 动物有利于 SPF 群体的成功建立和可持续发展，因为这些动物是 天然不含 SIV 和 SRV-D（待检测的四种病毒中的两种）。尽管如此，为了安全并根据需要 对于 SPF 计划，我们继续对所有四种病毒（B 病毒、STLV、SIV 和 SRV-D）进行系统测试。这 该计划提供具有明确遗传和病毒学背景的纯印度产恒河猴。 群体遗传分析显示，SPF 群体随着时间的推移一直保持遗传同质性 与其非 SPF 创始人相比，具有足够的杂合性和最小的分层。 我们计划在执行本核心提议的计划期间实现以下具体目标： 目标 1： 将封闭的 CPRC-SPF 印度源恒河猴群落维持在稳态水平，可以提供 高质量的动物来支持众多 NIH 资助的研究项目，并继续努力实现自我 充足性。 SPF 群体将按照监管机构的规定进行维护。目标 2. 使用 作为整体财务计划的一部分，预计恢复成本收入将用于支持 CPRC-SPF 殖民地。 在 NIH 的持续支持、机构捐款和计划收入的资助下，SPF CPRC 的计划仍将是重要的国家研究资源，并将能够提供健康的 SPF NIH 资助的研究中具有明确遗传和病毒学背景的印度恒河猴 程序。