MAINTENANCE OF A CLOSED CPRC SPF COLONY

封闭式 CPRC SPF 群体的维护

• 批准号: 10647880
• 负责人: Melween I. Martinez
• 金额: $ 367.32万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10647880
• 关键词: Acquired Immunodeficiency Syndrome; Admixture; Algorithms; Animals; Biological Models; Biomedical Research; Birth; Breeding; Budgets; Caribbean region; Caring; Characteristics; Chinese; Collaborations; Communities; Development; Disease; Education; Ensure; Funding; Genetic; Genetic Variation; Genomics; Geographic Locations; Goals; Grant; Guidelines; HIV/AIDS; Heterozygote; Human; Income; India; Institution; International; Intervention; Island; Macaca; Macaca mulatta; Maintenance; Medical; Medical center; Minority; Modeling; Monkeys; Population; Population Genetics; Population Sizes; Primates; Provider; Puerto Rico; Reporting; Reproduction; Research; Research Personnel; Research Project Grants; Resources; Retroviridae; SIV; Safety; Science; Secure; Simian Retroviruses; Solid; Stratification; Tail; Testing; Time; Training; United States National Institutes of Health; Universities; Vaccines; Viral; Virus; biomedical resource; cohort; driving force; effective therapy; genetic analysis; genetic approach; germ free condition; higher education; human disease; improved; offspring; operation; programs; recruit; research and development; therapeutic development

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. Our approach for genetic management will be concordant with the NIH's National NHP Genetics and Genomics Consortium guidelines for ensuring the long- term viability of the CPRC's SPF colony. The estimates of Indian ancestry among the SSFS SPF colony animals is also among the highest that have been calculated across several National Primate Research Centers (NPRCs) in the US. Funds are requested in this application to sustain the SPF program and to meet the proposed aims. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目概要/摘要： 此应用程序的主要目标是维持加勒比海地区的无特定病原体 (SPF) 计划 灵长类动物研究中心 (CPRC) 通过运营、管理、兽医护理、病毒学支持 MHC 分型以及我们综合遗传管理计划的改进。一个独特的特点是 CPRC 动物有利于 SPF 群体的成功建立和可持续发展，因为这些动物是 天然不含 SIV 和 SRV-D（待检测的四种病毒中的两种）。尽管如此，为了安全并根据需要 对于 SPF 计划，我们继续对所有四种病毒（B 病毒、STLV、SIV 和 SRV-D）进行系统测试。这 该计划提供具有明确遗传和病毒学背景的纯印度产恒河猴。 群体遗传分析显示，SPF 群体随着时间的推移一直保持遗传同质性 与其非 SPF 创始人相比，具有足够的杂合性和最小的分层。遗传 使用本文中描述的遗传管理策略将在 SPF 群体中维持变异 应用。遗传管理方法将允许富集所需的 MHC 标记 用于生物医学研究，同时保持遗传多样性。我们的遗传管理方法将是 符合 NIH 国家 NHP 遗传学和基因组学联盟指南，以确保长期 CPRC SPF 群体的长期生存能力。 SSFS SPF 群体动物中印度血统的估计 也是多个国家灵长类研究中心 (NPRC) 计算出的最高值之​​一 在美国。本申请要求提供资金来维持 SPF 计划并实现拟议目标。 在 NIH 的持续支持、机构捐款和计划收入的资助下，SPF CPRC 的计划仍将是重要的国家研究资源，并将能够提供健康的 SPF NIH 资助的研究中具有明确遗传和病毒学背景的印度恒河猴 程序。