MAINTENANCE OF A CLOSED CPRC SPF COLONY

封闭式 CPRC SPF 群体的维护

基本信息

批准号：
9272956
负责人：
Melween I. Martinez
金额：
$ 365.7万
依托单位：
UNIVERSITY OF PUERTO RICO MED SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-01 至 2020-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9272956
关键词：
AIDS/HIV problem Acquired Immunodeficiency Syndrome Admixture Algorithms Animals Biological Models Biomedical Research Birth Breeding Budgets Caribbean region Caring Cercopithecine Herpesvirus 1 Characteristics Chinese People Collaborations Communities Development Disease Ensure Funding Funding Mechanisms Genetic Genetic Variation Genomics Geographic Locations Goals Grant Guidelines Heterozygote Human Income India Institution International Intervention Island Macaca Macaca mulatta Maintenance Medical Medical center Minority Monkeys Population Population Genetics Population Sizes Primates Provider Puerto Rico Recruitment Activity Reporting Reproduction Research Research Personnel Research Project Grants Resources SIV Safety Science Secure Simian Retroviruses Solid Stratification Testing Time Training United States National Institutes of Health Universities Vaccines Viral Virus biomedical resource cohort driving force effective therapy feeding genetic analysis genetic approach germ free condition higher education human disease improved model development offspring operation programs public health relevance research and development research study therapeutic development trait virology working group

项目摘要

DESCRIPTION (provided by applicant): The major goal of this application is to maintain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, management, veterinary care, virological and MHC typing, and by the improvement of the comprehensive genetic management plan. A unique feature of the CPRC animals that had facilitated the successful establishment and improved sustainability of the SPF colony is that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for(4). Undoubtedly, this trait has contributed to the establishment of a successful program with very limited number of sero-conversions. Nevertheless, for safety and as required for the SPF Program, The CPRC continues systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minima stratification from its non- SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. To ensure the long-term viability of the CPRC-SPF colony, the Center's approach for genetic management will be concordant with the NIH's National NHP Genetics and Genomics Working Group's guidelines. The estimate of Indian ancestry among the C P R C - S P F a n i m a l s is also among the highest that have been calculated across several National Primate Research Centers (NPRCs) in the US. Funds are requested in this application to maintain the SPF program and to meet the proposed aims. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF Indian- origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs, specially HIV/AIDS studies.

描述（由申请人提供）：本申请的主要目标是通过支持运营、管理、兽医护理、病毒学和 MHC 分型来维持加勒比灵长类动物研究中心 (CPRC) 的无特定病原体 (SPF) 计划，并通过全面遗传管理计划的改进促进了 SPF 群体的成功建立并提高了可持续性，这些动物天然不含 SIV 和 SRV-D 这两种病毒。毫无疑问，这一特征有助于建立一个血清转化数量非常有限的计划，但为了安全性和 SPF 计划的要求，CPRC 仍在系统地继续进行。对所有四种病毒（B 病毒、STLV、SIV 和 SRV-D）进行检测该计划为未混合的印度恒河猴提供了明确的遗传和病毒学背景。群体遗传分析显示，SPF 群体具有使用本申请中描述的遗传管理策略，将在SPF群体中保持来自其非SPF创建者的足够量的杂合性和最小分层的同质性。遗传管理方法将允许期望的MHC的遗传富集。为确保 CPRC-SPF 群体的长期生存能力，该中心的遗传管理方法将与 NIH 的国家 NHP 遗传学和基因组学工作组的指南对 CPR C-SP 动物中印度血统的估计也是美国多个国家灵长类研究中心 (NPRC) 计算出的最高值之一，本申请要求提供资金来维持 SPF 计划。为了实现拟议的目标，在 NIH 的持续支持、机构捐款和计划收入的资助下，CPRC 的 SPF 计划将仍然是重要的国家研究资源，并将能够为 NIH 资助的研究项目（特别是艾滋病毒/艾滋病研究）提供具有明确遗传和病毒学背景的健康 SPF 印度恒河猴。