A CAROLINA CENTER TO CHARACTERIZE & MAINTAIN MUTANT MICE

卡罗来纳州的特色中心

• 批准号: 7961189
• 负责人: TERRY R MAGNUSON
• 金额: $ 101万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7961189
• 关键词: A Mouse; Advertising; Archives; Biomedical Research; Communities; Computer Retrieval of Information on Scientific Projects Database; Cryopreservation; Development; ES Cell Line; Fertilization; Fertilization in Vitro; Funding; Future; Genes; Gnotobiotic; Grant; Human; Individual; Institution; Methods; Mouse Strains; Mus; Mutant Strains Mice; Phenotype; Point Mutation; Procedures; Research; Research Infrastructure; Research Personnel; Resources; Series; Services; Source; Technology; United States National Institutes of Health; experience; human disease; improved; interest; meetings; mouse genome; mutant; mutant mouse model; new technology; pathogen; repository

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansion ever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 描述（由申请人提供）： 由于最近的人类和小鼠基因组以及小鼠技术的进步，小鼠突变体的数量和人类疾病模型的数量大大增加。维持和分发无病原体的小鼠菌株既劳动密集型，又代价供各个研究人员维持长期。 NIH/NCRR认识到需要对转基因小鼠的共同存储库和配送中心，并在1999年通过资助了突变小鼠区域资源中心（MMRRC）的回应。 UNC-Chapel Hill是为了整个生物医学界的利益而开发此资源的四个MMRRC中心之一。自1999年最初的资金以来，已经为创建组织基础架构并建立标准操作程序（SOP）进行了大量努力，用于进口，重新培训，表型，冷冻保存和突变小鼠菌株的分布。 2001年，MMRRC-UNC接受了其第一个菌株，此后一直经历了显着的扩张。与这些努力同时，MMRRC还利用了UNC上可用的专业知识，以将新技术和资源带到社区，例如突变的ES-Cell系，Gnotobiotic菌株和改善体外受精方法。 To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5)在感兴趣的基因作为社区资源中生成一系列等位基因突变。