Skin Biology and Diseases Resource-based Center at Mount Sinai

西奈山皮肤生物学和疾病资源中心

• 批准号: 10463721
• 负责人: Elena Ezhkova
• 金额: $ 84.5万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10463721
• 关键词: ATAC-seq; Adoption; Advertising; Archives; Area; Award; Bioinformatics; Biology; Biometry; CRISPR/Cas technology; Candidate Disease Gene; Cells; ChIP-seq; Clinical; Collaborations; Communication; Communication Barriers; Communities; Complement; Consult; Consultations; Custom; Data; Data Analyses; Data Reporting; Dermatology; Development; Disease; Disease model; Educational process of instructing; Electronic Mail; Ensure; Environment; Epigenetic Process; Evaluation; Faculty; Faculty Recruitment; Financial Support; Fostering; Funding; Future; Gene Expression; Generations; Genes; Genomics; Geography; Goals; Grant; Health; Hi-C; High School Student; Hospitals; Human; Individual; Infrastructure; Institution; Interdisciplinary Communication; Internships; Junior Physician; Laboratories; Leadership; Mentors; Minority; Modeling; Molecular; Mus; NCI Scholars Program; National Institute of Arthritis and Musculoskeletal and Skin Diseases; New York; Patients; Persons; Physicians; Population Heterogeneity; Positioning Attribute; Research; Research Personnel; Resources; Sampling; Scholarship; Scientist; Services; Skin; Small RNA; Standardization; Structure; Study models; Technology; Testing; Therapeutic; Training; Training Support; Translations; Universities; analysis pipeline; base; combat; cost; data integration; data visualization; design; exome sequencing; genetic analysis; genome sequencing; human disease; improved; induced pluripotent stem cell; innovation; laboratory experience; member; minority children; minority scientist; minority student; mouse model; novel; outreach; programs; racial disparity; recruit; screening; single cell analysis; single-cell RNA sequencing; skin barrier; skin disorder; skin organogenesis; social media; success; transcriptome sequencing; transcriptomics; underserved minority; user-friendly; virtual; web site; whole genome

Summary - Overall The overall goal of the Skin Biology Resource-Based Center (SBDRC) at Mount Sinai is to accelerate research in skin biology and diseases and promote its translation to benefit human health. To achieve this, we will harness the technical innovations, high-end infrastructure and computing power available at Mount Sinai to specifically advance and innovate skin research. In parallel we will ensure a vibrant future for skin biology and diseases research by expanding our research community and increasing its diversity. Administrative Core A will provide management, structure and oversight; award User Scholarships to reduce cost barriers to high end technologies; communicate SBDRC programs through a website and social media; organize research talks, seminars and retreats to promote scientific interchange and collaborations; attract new investigators and support development of junior and physician-scientists through Pilot Grants and Individual Mentoring Committees; and improve diversity through Mount Sinai's newly established Laureates Program that facilitates targeted faculty recruitment, and by establishing the SBDRC Sinai Skin Scholars Program, which will introduce underserved East Harlem high school students to skin biology and diseases research. Our Resource Cores are designed to capitalize on Mount Sinai's institutionally-supported and highly innovative technological infrastructure and expertise. We will support effort for technological experts to prioritize SBDRC goals and immerse themselves in skin research labs. Each Resource Core will provide unique expertise from leaders in diverse research areas, and technology, training and services customized to users' needs. The 3 Resource Cores will interact seamlessly: Resource Core B, “Modeling of Skin Disease for Mechanistic Analysis and Therapeutic Discovery,” will facilitate access to human skin samples, and provide technology, consultation and assistance with multiplexed immunostaining, isolation of skin cell subpopulations, generation and analysis of genetic mouse models, and gene editing and gene expression manipulation in human iPSC-derived skin cells. Skin cells or samples from Core B will be analyzed at the molecular level in Resource Core C, “Skin Genomics, Transcriptomics, and Epigenetics Core,” which will provide access to genomics (exome sequencing, whole genome sequencing, and targeted capture), transcriptomics (RNA-seq, ISO-seq, small RNA seq), epigenetic (ATAC-seq, ChIP-seq, CUT&RUN, Hi-C), and single-cell (scRNA-seq, scATAC-seq, spatial transcriptomics) technologies. Resource Core D, “Data Analysis and Integration Core,” will provide standardized biostatistical and bioinformatics analysis and integration of Core C data and make these easily available through Skin-GLOW, a novel user-friendly interface for data visualization. In turn, data analyzed in Core D will yield candidate genes and mechanisms that will be tested through functional analyses provided in Core B. Our plans will create a productive, impactful and extensive skin Research Community including strong external hubs at NYU and at Yale University.

摘要 - 总体 西奈山（Mount Sinai）基于皮肤生物学资源中心（SBDRC）的总体目标是加速研究 在皮肤生物学和疾病中，并促进其翻译以使人类健康受益。为了实现这一目标，我们将 利用西奈山可用的技术创新，高端基础设施和计算能力 具体推进和创新的皮肤研究。同时，我们将确保皮肤生物学和 通过扩大我们的研究界并增加其多样性来进行疾病研究。行政核心a 将提供管理，结构和监督；授予用户奖学金，以将成本障碍降低到高端 技术；通过网站和社交媒体传达SBDRC程序；组织研究谈判， 下水道和务虚会，以促进科学交换和合作；吸引新的调查员和 通过飞行员赠款和个人指导来支持初级和身体科学家的发展 委员会；并通过西奈山新成立的获奖计划来改善多样性，以促进 有针对性的教师招聘，并通过建立SBDRC西奈皮肤学者计划，该计划将引入 服务不足的东哈林高中生从事皮肤生物学和疾病研究。我们的资源核心 旨在利用西奈山的机构支持和高度创新的技术 基础设施和专业知识。我们将支持技术专家的努力，以优先考虑SBDRC目标和 沉浸在皮肤研究实验室中。每个资源核心将为领导者提供独特的专业知识 各种研究领域以及针对用户需求定制的技术，培训和服务。 3个资源 核心将无缝相互作用：资源核心B，“用于机械分析和 治疗发现，“将有助于获取人类皮肤样本，并提供技术，咨询 并有助于多重免疫染色，皮肤细胞亚群的隔离，生成和分析 遗传小鼠模型以及人IPSC衍生的皮肤中的基因编辑和基因表达操纵 细胞。来自核心B的皮肤细胞或样品将在资源核心C的分子水平上进行分析，“皮肤 基因组学，转录组学和表观遗传学核心”将提供基因组学的访问 测序，整个基因组测序和靶向捕获），转录组学（RNA-SEQ，ISO-SEQ，小 RNA SEQ），表观遗传（ATAC-SEQ，CHIP-SEQ，CUT＆RUN，HI-C）和单细胞（SCRNA-SEQ，SCATAC-SEQ， 空间转录组学）技术。资源核心D，“数据分析和集成核心”，将提供 标准化的生物统计和生物信息学分析和核心C数据的整合 可通过Skin-Glow获得，这是一种新型的用户友好界面，用于数据可视化。反过 核心D将产生候选基因和机制，这些机制将通过提供的功能分析进行测试 核心B.我们的计划将创建一个富有成效，有影响力和广泛的皮肤研究社区 纽约大学和耶鲁大学的外部枢纽。