A Carolina Center to Characterize & Maintain Mutant Mice

卡罗来纳州中心的特色

• 批准号: 7909772
• 负责人: TERRY R MAGNUSON
• 金额: $ 24.66万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-04 至 2012-03-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7909772
• 关键词: Carolina; Center; Characterize; Maintain; Mutant

Due to the recent completion of the human and mouse genomes as well as advances in mouse technologies, there has been an enormous increase in the number of mouse mutants and models for human disease. Maintaining and distributing pathogen-free mouse strains is both labor-intensive and costly for individual investigators to sustain longterm. The NIH/NCRR recognized the need for a common repository and distribution center for genetically modified mice, and in 1999 they responded by funding the Mutant Mouse Regional Resource Centers (MMRRC). UNC-Chapel Hill is one of four MMRRC centers that are developing this resource for the benefit of the entire .biomedical community. Since the initial funding in 1999, a significant amount of effort has been invested in creating the organizational infrastructure and establishing standard operating procedures (SOPs) for importation, rederivation, phenotyping, cryopreservation and distribution of mutant mouse strains. In 2001, the MMRRC-UNC accepted its first strain and has experienced significant-expansionever since. In parallel with these efforts, the MMRRC has also taken advantage of the expertise available at UNC to bring new technologies and resources to the community such as mutant ES-cell lines, gnotobiotic strains and improved in vitro fertilization methods. To meet future demands for MMRRC services and improve upon existing technologies, this proposal aims to: (1) import, archive and distribute genetically modified mouse strains, (2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community, (3) develop improved and reliable methods for assisted fertilization, (4) integrate gnotobiotic strain development into MMRRC services (5) generate an allelic series of point mutations in genes of interest as a community resource.

由于人类和小鼠基因组最近完成以及小鼠的进步 技术，人类的小鼠突变体数量和模型的数量大大增加 疾病。维持和分发无病原体的小鼠菌株既劳动密集型又昂贵 个人调查人员长期维持。 NIH/NCRR认识到需要公共存储库 和转基因小鼠的配送中心，并在1999年通过资助突变体做出反应 小鼠区域资源中心（MMRRC）。 UNC-Chapel Hill是四个MMRRC中心之一 为整个生物医学界的利益开发此资源。自1999年最初的资金以来 已经投入了大量努力来创建组织基础架构并建立 用于进口，重新培训，表型，冷冻保存和的标准操作程序（SOP） 突变小鼠菌株的分布。在2001年，MMRRC-unc接受了其第一个菌株，并具有 从那以后，经历了大量超越。与这些努力并联，MMRRC也采取了 UNC可用的专业知识的优势将新技术和资源带到社区 作为突变的ES细胞系，gnotobiotic菌株和改善的体外受精方法。满足未来 对MMRRC服务的需求并改进了现有技术，该建议的目的是：（1）进口， 档案和分布转基因的小鼠菌株，（2）通过 广告和招标针对生物医学研究界，（3）发展了改进且可靠的 辅助施肥的方法，（4）将gnotobiotic菌株的发育整合到MMRRC服务中（5） 在感兴趣的基因作为社区资源中生成一系列等位基因突变。